A STUDY ON DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF CEFPODOXIME IN BULK AND A PHARMACEUTICAL DOSAGE FORMS

Main Article Content

S. K. SABEEHA BEGUM
N. BARATHA JYOTHI
R. TULASI
R. SARALA
G. SRAVANI

Abstract

A simple, rapid, precise, sensitive and, reproducible reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the quantitative analysis of Cefpodoxime in a pharmaceutical dosage form. Chromatographic separation of Cefpodoxime was achieved on Waters Alliance -2695, by using Luna Pheny Hexyl (250mm x 4.6mm, 5µm) column and the mobile phase containing 0.1% TEA adj pH-2.5 with OPA & ACN in the ratio of 75:25% v/v. The flow rate was 1.0 ml/min, detection was carried out by absorption at 222nm using a photodiode array detector at ambient temperature. The number of theoretical plates and tailing factor for Cefpodoxime were NLT 2000 and should not be more than 2 respectively. The linearity of the method was excellent over the concentration range 7-105 µg/ml for Cefpodoxime respectively. The correlation coefficient was 0.999. % Relative standard deviation of peak areas of all measurements always less than 2.0. The proposed method was validated according to ICH guidelines.  The method was found to be a simple, economical, suitable, precise, accurate & robust method for quantitative analysis of Cefpodoxime and study of its stability.

Keywords:
HPLC, Cefpodoxime, stability, pharmaceutical dosage

Article Details

How to Cite
BEGUM, S. K. S., JYOTHI, N. B., TULASI, R., SARALA, R., & SRAVANI, G. (2021). A STUDY ON DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF CEFPODOXIME IN BULK AND A PHARMACEUTICAL DOSAGE FORMS. Asian Journal of Advances in Medical Science, 3(4), 290-299. Retrieved from http://mbimph.com/index.php/AJOAIMS/article/view/2612
Section
Original Research Article

References

Laurenc L Brunton, John S Lazo, Keith L. Parker. ‘Goodman and Gilman’s The pharmacological basis of Therapeutics; McGraw Hill, Newyork, U.S.A. 2006;11:1136-1142.

O’Neil MJ. The Merk Index- an encyclopedia of chemicals, Drugs and Biologicals, New Jersy, Merk and co., INC. 1917;13:159.

Indian Pharmacopoeia. Indian Pharmacopoeia commission, Ghaziabad. 2010;2:149,857,151, 1018.

British Pharmacopoeia. London, Medicines and Health care products Regulatory Agency (MHRA). 2005;3:192.

John HB, John MB. Wilson and gisvold’s textbook of organic medicinal & pharmaceutical chemistry, lippincott williams & wilkins, wolterskluner company. 2004;11:330.

Sean C. Sweetman, martindale the complete references; pharmaceutical press, 1 Lambeth High Street, London SEI 7 IN, UK. 2002;33:172.

Darji B H, NJ Shah, AT Patel, NM Patel. Development and validation of a HPTLC method for the estimation of cefpodoxime proxetil. Indian J. of Pharm. Sci. 2007;69(2):331-3.

Siddalinga Swamy MS, Sathish Kumar Shetty A, Anil kumar SM. UV-Visible spectrophotometric methods for the estimation of cefpodoxime proxetil in bulk drug and pharmaceutical dosage form. Int.J. PharmTech Res. 2012;4(2):750-6.

Narendra Nyola, Govindasamy Jeyabalan. Simultaneous estimation of Cefixime And Azithromycin in API’S and pharmaceutical dosage form by RP-HPLC. Indo American. J.of Pharm Res. 2013;2(12):1472-81.