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The present study was undertaken to investigate the impacts of the two essential oils; Syzygium aromaticum (Clove) and Pinus brutia (Turpentine) against 1-3 days old eggs of pink bollworm Pectinophora gossypiella; the toxicity and the adverse impact of these products on some biological and physical characters were studied under laboratory conditions at 25 ±1 °C and 65±5% R.H.
The obtained results revealed that the eggs of P. gossypiella pest were highly susceptible to Pinus than Syzyginum. The LC50 value were 9.38% ppm when PBW eggs treated with Turpentine oil, while it increased to 11.57% when 1-3 days old eggs of P. gossypiella dipping with Syzyginum. Some biological aspect studies show a prolongation in larval and pupal stages developments resulted from treated 1-3 day old eggs by S. aromaticum and P. brutia.It estimated by 9.0 and 20.0 days/ larvae, respectively for larvae and 9.3 and 10.2 days/ pupae. . The life cycle were significant affected, as it was elongated to 34.1 and 36.9, respectively, compared with 26.2 days in control.
In contrast, in adult stage, the results indicated that the percentage of adult emergence was highly decreased to 56.0 and 61.0% emerged from eggs treated with LC50 of Syzygium and Turpentine, respectively, compared with (94.00%) control, with high reduction in total eggs laid, percentage of hatchability. The average number of eggs deposited by females were 107 and 97 eggs laid at 11.1 and 7.6 days oviposition/ female, resulted from Syzygium and Turpentine, respectively compared with 226.0 eggs/ female in control deposited at 11.6 days.
The physical result indicated that latent adverse effect in biochemical larvae resulted from eggs treated with two essential oil Syzygium and Turpentine. It caused high decreased in total protein to 15.0 and 8.6 (total protein (mg/g.b.wt.), respectively compared with17.0 mg/g.b.wt. /larvae in control, with decreased the Phenoloxidase, to 14.8 and 13.5 (O.D. units/g.b.wt), respectively, compared with 26.0 Phenoloxidase (O.D. units/g.b.wt) in control. In contrast, increased in free-amino acid to 137.3 and 298.0 (µg D, L- alanine/g.b.wt) respectively, compared with 118.0 in control, also, the total lipid increased to 27.9 and 22.0 (mg/g.b.wt.), respectively, compared with16.9 (mg/g.b.wt.) in control.
Thonggoom O, Punrattanasin N, Srisawang N, Promawan N, Thonggoom R. In vitro controlled release of clove essential oil in self-assembly of amphiphilic polyethylene glycol-block-polycapro-lactone. J Microencapsul. 2016;33(3):239–248. [PubMed] [Google Scholar]
Sebaaly C, Charcosset C, Stainmesse S, Fessi S, and Greige G H. Clove essential oil-in-cyclodextrin-in-liposomes in the aqueous and lyophilized states: From laboratory to large scale using a membrane contactor. Carbohydr Polym. 2016;138:75–85. [PubMed] [Google Scholar].
Tripathi AK, Prajapati V, Aggarwal KK, Khanuja SBS and Kumar S. Repellency and toxicity of oil from Artemisia annual to certain stored product beetles. J. Econ. Entomol. 2000; 93(1): 43-47.
Choi WL, Lee SG, Park HM and Ahn YJ. Toxicity of plant essential oil to Tetranychus urticae (Acari: Tetranchidae) and Phytoseiulus persimilis (Acari: phytoseiidae). J. Econ. Entomol. 2004;97:553-558.
Alejandro LP, Audno G and Masur H. Larvicidal effect of Eucalyptus Grandis essential oil and turpentine and their major components on Aedes egypti larvae Journal of the American Mosquito Control Association. 2007; 23-3:299-303
Rofail, MM, Nada AM, El-Sisi G and Rashad AM. Time of spraying some natural oils as a limiting factor for controlling cotton bollworm, Pectinophora gossypiella (Saunders). Egypt. J. Agric. Res. 2000;78:4- 1499-1507.
Fayemiwo K A, Adeleke MA, Okoro OP, Awojide SH and Awoniyi IO. Larvicidal efficacies and chemical composition of essential oils of Pinus sylvestris and Syzygium aromaticum against mosquitoes. Asian Pac J Trop Biomed. 2014;4 -1-30–34. [PMC free article] [PubMed] [Google Scholar]
Zhang S, Xiao-zhen YE. Impacts of chemical insecticides on extracellular protease and chitinase activities of Metarhizium anisopliae. J. Fujian College Forest. 2010;4:289-292.
Zeng L, Lao CZ, Cen VG, Zeng and liang GW. Study on the insecticidal activity compounds of the essential oil from Syzygium aromaticum against stored grain insect pests.: 2010; Proceedings of the 10th International Working Conference on Stored Product Protection
Mohammad M, Izadi H. Cooling rate and starvation affect supercooling point and cold tolerance of the Khapra beetle, Trogoderma granarium everts fourth instar larvae (Coleoptera: Dermestidae). J. Therm. Biol. 2018;71: 24–31.
Kandil Mervat AA, EL-Shennawy RA, Ahmed DA. Arbohydrate eydrolyzing enzymes as a target for Pink Bollworm Pectinophora gossypiella (Saund.) Control agents. J. of Mansoura; 2020.
Amer AEA. Economic artificial diets for rearing spiny bollworm, Earias insulana (Boisd.) (Lepidoptera: Noctuidae). J. Plant Prot. and Path., Mansoura Univ. 2015;6(3):527 – 534.
Jirovetz L, Buchbaue Gr, Stoilova I, Stoyanova S, Krastanov A, Erich S. Chemical composition and antioxidant properties of clove leaf essential oil. J Agric Food Chem. 2006; 23;54 (17):6303-7.
Snedecor GW. Statistical methods 5th Ed, Iowa State Col.N.Y.; 1952.
Duncan DB. Multiple range and multiple F test. Biometrics. 1955;11:1-42.
Bradford MM. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein dye binding. Anal.Biochem.1976;72:248- 254.
Ishaaya, I. Observation on the phenoloxidase system in the armored scale Aonidiella aurantii and Chrysomphalus aonidum. Comp. Biochem. Physiol. 1971;39 B:935-943.
Mina M, andKhalequzza M. Toxicity of essential oils against red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). bio-sci. 2007; 17: 57.
Mina M, and Khalequzza M. Ovicidal activity of essential oils against red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae). J. bio- sci. 2009;17:57-62, 2
El-Mesallamy AMD, Raslan SA, Nagar ME, El-Medany WAZ. Toxicity and latent effect of pepper plant essential oil (Capsicum annum) on Some Cotton Pests. Middle East Journal of Applied Sciences. 2015;2077-4613-5; 1184-1191
Cruz GS, Wanderley TV, Oliveira V, Correia AA, Breda MO, Alves TGS, Cunha FM, Teixeira AAC, Dutra KA, Navarro DMAF. Bioactivity of Piper hispidinervum (Piperales: Piperaceae) and Syzygium aromaticum (Myrtales: Myrtaceae) oils, with or without formulated Bta on the Biology and Immunology of Spodoptera frugiperda (Lepidoptera: Noctuidae). J. Econ. Entomol. 2014;107 - 1:144-153.
Ahmed DA. Comparative studies, under laboratory conditions, of four selected insecticides on Pink Bollworm, Pectinophora gossypiella (Saund.). Egypt. Acad. J. Biolog. Sci. (F. Toxicology and Pest control). 2020; 12(2):47- 61.
Kandil MA, Fouad EA, Hefny DE and Abdel-Mobdy YE. Toxicity of fipronil and emamectin benzoate and their mixtures against cotton leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae) with relation to GABA content. Journal of Economic Entomology. 2020;113: 385–389.
Yacoub SS. Efficacy of some plant extracts in controlling Sesamia cretica Led. and Ostrinia nubilalis (Hubn.) in maize fields. Ph.D. Thesis, Fac. Agric., Benha Univ. 2006;289.
Żółtowska K, Lipiński Z, Łopieńska-Biernat E, Farjan M and Dmitryjuk M. The activity of carbohydrate-degrading enzymes in the development of brood and newly emerged workers and drones of the Carniolan Honeybee, Apis mellifera carnica. J. Insect Sci. 2012;12(1):Article 22. Available:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471771/.
kandil, Mervat AA, Ahmed F and Moustafa Hemat Z. Toxicological and biochemical studies of lufenuron, chlorfluazuron and chromafenozide against Pectinophora gossypiella (Saunders). Egypt. Acad. J. Biolog. Sci. 2012;1: 37- 47.
Kandil Mervat AA, Salem MS and Adly AM. Biological and biochemical changes in pink bollworm, Pectinophora gossypiella after treatment with Hexaflumuron and Chlorfluazuron. Annals of Agric. Sci. Moshtohor. 2013;51-4- 427- 432.
Costat Statistical Software. Microcomputer program analysis version. CoHort Software, Monterey, California. 1990;6:311.
Kanat M and Alma MH. insecticidal effects of essential oils from various plants against larvae of pine processionary moth (Thaumetopoea pityocampa Schiff) (Lepidoptera: Thaumetopoeidae). Pest Management, Sci., 2004;60(2):173-177.
Magda S and Shadia A. Roll of three essential oils and their Nano against Ephestia cautella (Lepidoptera-Pyralidae) under laboratory and store conditions /International Journal of Pharm Tech Research. 2016;9(10):194-200.