Main Article Content
Biodegradation by natural population of microorganisms represents one of the cheap primary mechanisms by which petroleum and other hydrocarbon pollutants can be removed from the environment. The effectiveness of Pseudomonas putida and Staphylococcus auerus in remediation of soil contaminated with spent engine oil was investigated using standard methods. The result indicates significant variation in bacteria count between spent engine oil contaminated soil and control soil in 3rd to 6th week of bioremediation (p˂0.05). pH of control soil was significantly (P˂0.05) different from bio-remediated soil in the 1st, 4th, 5th and 7th week of bioremediation. Percentage organic matter content of control soil also differed significantly from bio-remediated soil in the 1st, 2nd, 3rd, 6th and 7th week of the experiment while the organic matter content of both samples did not show any significant difference in the 4th and 5th week (P˂0.05). There was no statistically significant difference between concentrations of Pb, Cu, and Zn in bio-remediated soil when compared with control soil (P˂0.05). Similarly total organic carbon in bio-remediated soil was not significantly different from the control soil (p=0.001). Pseudomonas putida and Staphylococcus aureus are effective in the clean-up of spent engine oil contaminated soil.
Kastner M, Miltner A. Application of compost for effective bioremediation of organic contaminants and pollutants in soil. Appl Microbiol Biotechnol. 2016;100:3433-49.
Amund OO, Adebowale AA, Ugoji EO. Occurrence and characteristics of hydrocarbon utilizing bacteria in nigerian soils contaminated with spent motor oil. Indian Journal of Microbiology. 1987;27:63-67.
Yan L, Penttinen P, Mikkonen A, Lindstrom K. Bacterial community changes in response to oil contamination and perennial crop cultivation. Environ Sci Pollut Res Int. 2018;12:018- 1635.
Adenipekun CO, Isikhuemhen OS. Bioremediation of engine oil polluted soil by the tropical white rot fungus, Lentinus squarrosulus Mont. (Singer). Pak J Biol Sci. 2008;11:1634-7.
Azubuike CC, Chikere CB, Okpokwasili GC. Bioremediation techniques-classification based on site of application: principles, advantages, limitations and prospects. World J Microbiol Biotechnol. 2016;32:016-2137.
Chen M, Xu P, Zeng G, Yang C, Huang D, Zhang J. Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons, petroleum, pesticides, chlorophenols and heavy metals by composting: Applications, microbes and future research needs. Biotechnol Adv. 2015;33:745-55.
Wawra A, Friesl-Hanl W, Jager A, Puschenreiter M, Soja G, Reichenauer T, Watzinger A. Investigations of microbial degradation of polycyclic aromatic hydrocarbons based on (13)C-labeled phenanthrene in a soil co-contaminated with trace elements using a plant assisted approach. Environ Sci Pollut Res Int. 2018;25:6364- 6377.
Wang SJ, Wang X, Lu GL, Wang QH, Li FS, Guo GL. Bioremediation of petroleum hydrocarbon-contaminated soils by cold-adapted microorganisms: research advance. Ying Yong Sheng Tai Xue Bao. 2011;22: 1082-8.
Nalini S, Parthasarathi R. Biosurfactant production by Serratia rubidaea SNAU02 isolated from hydrocarbon contaminated soil and its physico-chemical characterization. Bioresour Technol. 2013;147:619-22.
He J, He C, Chen X, Liang X, Huang T, Yang X, Shang H. Comparative study of remediation of Cr(VI)-contaminated soil using electrokinetics combined with bioremediation. Environ Sci Pollut Res Int. 2018;18:018- 1741.
Wang J, Li X, Li X, Wang H, Su Z, Wang X, Zhang H. Dynamic changes in microbial communities during the bioremediation of herbicide (chlorimuron-ethyl and atrazine) contaminated soils by combined degrading bacteria. PLoS One. 2018;13.
Cecotti M, Coppotelli BM, Mora VC, Viera M, Morelli IS. Efficiency of surfactant-enhanced bioremediation of aged polycyclic aromatic hydrocarbon-contaminated soil: Link with bioavailability and the dynamics of the bacterial community. Sci Total Environ. 2018; 634:224-234.
Kong FX, Sun GD, Liu ZP. Degradation of polycyclic aromatic hydrocarbons in soil mesocosms by microbial/plant bioaugmen-tation: Performance and mechanism. Chemosphere. 2018;198:83-91.
Sharma S, Tiwari S, Hasan A, Saxena V, Pandey LM. Recent advances in conventional and contemporary methods for remediation of heavy metal-contaminated soils. 2018;8:018-1237.
Bahuguna A, Lily MK, Munjal A, Singh RN, Dangwal K. A study of the physicochemical analysis of automobile contaminated soil of Uttarakhand, India. International Journal of Environmental Sciences. 2012;2:2.
Mbachu AE, Chukwura EI, Mbachu NA. Waste engine oil degrading potentials of indigenous fungi isolated from auto-mechanic workshops: Impacts of heavy metals (Zn and Pb) co- contamination and pH. American Journal of Life Science Researches. 2017;5(1): 6-17.
Ayandele AA. Microbial treatment of soil contaminated with spent engine oil / biotreatment of soil contaminated with spent engine by microorganisms. 2018;20.
Oyeleke SB, Manga BS. Essentials of laboratory practical in microbiology, First Edition ed., Niger State: Tobest publisher. 2008;36-60.
Rabah AB, Ibrahim ML. Physicochemical and microbiological characterization of soils laden with tannery effluents in Sokoto, Nigeria, Nigerian Journal of Basic and Applied Science, 2010;18(1):65-71.
Aligwekwe IA. Physicochemical and biochemical characterization of asphalt plant dumpsite located at Obinze, Imo State, Nigeria; 2018.
Nirmal Kumar JI, Soni HB, Bhatt I. Macrophytes in phytoremediation of heavy metal contaminated water and sediments in Parieyej Community Reserve, Gujarat, India. Turkish Journal of Fisheries and Aquatic Sciences. 2008;193-200.
Fawole MO, ỌsỌ BA. Laboratory manual of microbiology. 5th Edition ed., Ibadan: Spectrum Books Limited. 2007; 15-35.
Suryakanta. How to determine organic matter content in soil, CivilBlog.org; 2015.
Dasgupta D, Brahmaprakash G. Soil microbes are shaped by soil physico-chemical properties: A brief review of existing literature. Inter-national Journal of Plant & Soil Science. 2021;59-71.
Lopes LD, Hao J, Schachtman DP. Alkaline soil pH affects bulk soil, rhizosphere and root endosphere microbiomes of plants growing in a Sandhills ecosystem. FEMS Microbiology Ecology, 97, fiab028; 2021.
Haritash A, Kaushik C. Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): A review, J. Hazard. Mater. 2009;169(1-3):1–15.
Song YQ, Shahir S, Abd Manan F. Bacterial inoculant-assisted phytoremediation of heavy metal-contaminated soil: Inoculant development and the inoculation effects. Biologia. 2021;1-11.
Rawat K, Yadav AK. Advances in biological techniques for remediation of heavy metals leached from a fly ash contaminated ecosphere. Pollutants and Water Management: Resources, Strategies and Scarcity. 2021;151-171.
Zhang C, Wu D, Ren H. Bioremediation of oil contaminated soil using agricultural wastes via microbial consortium. Scientific Reports. 2020; 10:1-8.
Ławniczak Ł, Woźniak-Karczewska M, Loibner AP, Heipieper HJ, Chrzanowski Ł. Microbial degradation of hydrocarbons—basic principles for bioremediation: A review. Molecules. 2020;25:856.
Zhang X, Bao D, Li M, Tang Q, Wu M, Zhou H, Liu L, Qu Y. Bioremediation of petroleum hydrocarbons by alkali–salt‐tolerant microbial consortia and their community profiles. Journal of Chemical Technology & Biotechnology. 2021;96:809-817.
Weiman S, Joye SB, Kostka JE, Halanych KM, Colwell RR. GoMRI insights into microbial genomics and hydrocarbon bioremediation response in marine ecosystems. Oceanography. 2021;34:124-135.