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The present study was carried out to examine the bioactive chemicals in methanolic stem bark extract of Indigofera zollingeriana using Gas Chromatography – Mass Spectrum (GC-MS). The result revealed the presence of 19 compounds with peak percentage in chromatogram. The major phytoconstituent in the exatract are: Indigoferabietone (21.67 %), Indigo, 2, 2-bisindole (14.35 %) and Indigoidin (12.49 %) with retention time of 17.38 min, 20.57 min and 10.33 min respectively. It was concluded that the presence of various bioactive chemicals or phytochemicals in Indigofera zollingeriana stem bark justifies that it can be used for the treatment of many diseases in animals and could be used as an alternative to antibiotics to promote food safety.

GC-MS analysis, Indigofera zollingeriana stem bark, methanol, phytochemicals

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ALAGBE, J. O., SHITTU, M. D., & USHIE, F. T. (2021). GC-MS ANALYSIS OF METHANOLIC STEM BARK EXTRACT OF Indigofera zollingeriana. Asian Journal of Advances in Research, 11(4), 144-146. Retrieved from http://mbimph.com/index.php/AJOAIR/article/view/2620
Original Research Article


Owolabi J, Omogbai EKI, Obasuyi O. Antifungal nd antibacterial activities of the ethanolic and aqueous extract of Kigelia africana stem bark. African Journal of Biotechnology. 2007;6:882-885.

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Taj UR, Muhammad AZ, Wajiha L, Muhammad S, Sajjad H, Iqbal CM. Phytochemistry and pharmacology of genus Indigofera: A review. Rac. Nat. Prod. 2018;12:(2018):1-13.

Ming KJ, Khang GL, Fatt CT. Recent advances in traditional plant drugs and orchids. Acta Pharmacology Sinica. 2003;24:7-21.

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Ojewuyi OB, Ajiboye TO, Adebanjo EO, Balogun A, Mohammed AO. Proximate composition, phytochemical and mineral contents of young and mature Polyalthia longifolia Sonn. Leaves. Fountain Journal of Natural and Applied Sciences. 2014;3(1):10-19.

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