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The present study was carried out to examine the bioactive chemicals in methanolic stem bark extract of Indigofera zollingeriana using Gas Chromatography – Mass Spectrum (GC-MS). The result revealed the presence of 19 compounds with peak percentage in chromatogram. The major phytoconstituent in the exatract are: Indigoferabietone (21.67 %), Indigo, 2, 2-bisindole (14.35 %) and Indigoidin (12.49 %) with retention time of 17.38 min, 20.57 min and 10.33 min respectively. It was concluded that the presence of various bioactive chemicals or phytochemicals in Indigofera zollingeriana stem bark justifies that it can be used for the treatment of many diseases in animals and could be used as an alternative to antibiotics to promote food safety.
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