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Diesel oil pollution is a major challenge in most developing countries like Nigeria. This study was carried out to ascertain the possibility of remediating diesel oil polluted soils using palm bunch ash. Samples of garden soil with no history of diesel oil pollution were spiked with 100mL of Bonny Light diesel oil and left for two weeks to simulate a condition of major spill before adding different weights of palm bunch ash (0, P+NOPBA, 50g, 150g, 250g and 350g). 1g from each treatment was collected monthly and subjected to laboratory analysis using standard analytical methods for soil selected heavy metals contents using AAS. Preliminary results revealed alteration of chemical properties of soils, elevated heavy metals levels two weeks after spiking indicating that the soil sample had undergone slight alteration. There was a dose dependent decrease in heavy metal content of the diesel oil polluted soils with time. Mean concentration of Zinc ranged from 95.7mg/kg to 7.45mg/kg; 9590.5 to 99.83 in Fe; 3.5mg/kg 1.4mg/kg Cu; 1.45mg/kg to 0.06mg/kg Cd and 10.90mg/kg to 0.05mg/kg in Pb. Overall, net reduction in heavy metals was very low in soil left under natural attenuation (polluted untreated soil) than treated soils. This study has revealed a marked degradation of the heavy metal content of soil which indicated that palm bunch ash could be used for remediation of diesel oil polluted soil. It is recommended that palm bunch ash be replaced with conventional fertilizer in restoration of crude contaminated soil.

Diesel oil, palm bunch ash, biodegradation, polluted, soil

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Onuoha EM, Anukwa FA, Nkang A, Nkeruwem J. Soil amendment potential of liquid and organo-mineral fertilizer on spent engine oil-polluted soil. Res. J. Soil Biol. 2019;12:1-8.

Onuoha EM, Ekpo IA, Anukwa FA, Nwagu KE. Microbial stimulating potential of Pineapple peel (Ananas comosus) and Coconut (Cocos nucifera) husk char in crude-oil polluted soil. Int. J. Enviorn. Agric. Biotechnol. 2020;5:582-593.

Boonyapookana B, Parkplan P, Techapinyawat S, DeLaune RD, Jugsujinda A. Phytoaccumulation of lead by sunflower (Helianthus annuus), tobacco (Nicotiana tabacum) and vetiver (Vetiveria zizanioides). J. Environ. Sci. Health A Tox. Hazard. Subst. Environ. Eng. 2005;40:117-137.

Wang Y. Phytoremediation of mercury by terrestrial plants. Ph.D. dissertation, Botaniska institutionen, Stockholm; 2004. Available:

Abioye OP, Ekundayo OP, Aransiola SA. Bioremoval of zinc in polluted soil using Acalypha inferno. Res. J. Environ. Sci. 2015;9:249-255.

Shmaefsky BR. Principles of Phytoremediation. In: Phytoremediation, Shmaefsky B. (Ed.). Springer International Publishing, Cham. 2020;1-26. ISBN: 978-3-030-00099-8.

McCutcheon SC, Schnor JL. Phytoremediation: Transformation and Control of Contaminants. Environmental and Science and Technology. A Joun and Sons, Inc., Wiley-Interscience, New Jersey, USA; 2003.

Wuana RA, Okieimen FE. Heavy metals in contaminated soils: A review of sources, chemistry, risks and best available strategies for remediation. ISRN Ecol; 2011.

Uhegbu FO, Akubugwo EI, Iwealab EJ, Uhegbu OC. Impact of spent engine oil on soil and the growth of Zea mays seeds. Sci. J. Environ. Sci. 2012;1:1-8.

Marinescu M, Toti M, Tanase V, Plopeanu G, Calciu L. The effect of crude oil pollution on physical and chemical characteristics of soil. Research Journal of Agricultural Science. 2011;43(3):125 –129.

Navarro-González R, Rainey FA, Molina P, Bagaley DR, Hollen BJ, de la Rosa J, Small AM, et al. Mars-like soils in the Atacama desert, Chile, and the dry limit of microbial life. Science. 2003;302(5647):1018–1021.

Nester E, Denisr NG, Anderson C, Evans RJR, Nancy N, Martha T. Microbiology a human perspective.3rd edition. 1991;17-23.

Nester EW, Anderson DG, Roberts CE, Pearsall NN, Nester MT. Microbiology: A human perspective. 4thedn., McGraw-Hill: New York, NY; 2004.

Nguyen C. Rhizodeposition of organic C by plants: mechanisms and controls. Agronomie. 2003;23(5/6):375–396.

Nicholson DT, Nicholson FH. Physical deterioration of sedimentary rocks subjected to experimental freeze–thaw weathering. Earth Surface Processes and Landforms. 2000;25(12):1295–1307.

Nwaogu LA, Onyeze GOC, Nwabueze RN. Degradation of diesel oil in polluted soil using Bacillus subtilis.African Journal Biotechnology. 2008;7(12):1939-1943.

Oades JM. The role of biology in the formation, stabilization and degradation of soil structure.Geoderma. 1993;56(1–4):377–400.

Oberdorster E, Cheek AO. Gender benders at the beach, endocrine disruption in marine and estuarine organisms. Environmental Toxicological Chemistry. 2000;20(4):23-36.

Obi CO, Nnabude PC, Onuoha E. Effect of kitchen waste compost and tillage on soil chemical properties and yield of okra (Abelmoschus esculentus).Nigerian Journal of Soil Science. 2005;15(2):69-76.

Obire O, Anyanwu EC, Okigbo RN. Saprophytic and crude oil degrading fungi from cow dung and poultry droppings as bioremediating agents. Journal of Agricultural Technology. 2008;4(92):81-89.

Odijimi AI, Oghalu MR. Remediation of petroleum hydrocarbon polluted systems: Exploiting the bioremediation strategies. African Journal of Biotechnology. 2006;5(25):2520-2525.

Odokuma LO. The Genius in the Microbes: An indispensable Tool for the Management of Xenobiotic Mediated Environmental Flux. University of Port Harcourt inaugural lecture. 2012;87.

Odokuma LO, Erenee BF, Solomon L. Biodegradability of lubricating fluids. Paper presented at the 38th Annual General Meeting and Scientific Conference of Nigerian Society for Microbiology (NSM), Multiple Hall, University of Lagos. 1st – 5th September, (BRO7). 2015;219.

Odu CTI, Nwobishi LC, Esuruoso OF, Ogunwale JA. Environmental study of the Nigerian Agip Company Operational Areas in the petroleum industry and the Nigerian Environment.Proceedings FMW and H and NNPC Conference. 1985;274-283.

Odum HT. Back Ground of Published Studies on Lead and Wetland. In: Howard T. Odum (Ed), Heavy Metals in the Environment Using Wetlands for Their Removal, Lewis Publishers, New York USA. 2000;32.

Ofoegbu RU, Momoh YOL, Nwaogazie IL. Bioremediation of Crude Oil Contaminated Soil Using Organic and Inorganic Fertilizers. Journal of Petroleum & Environmental Biotechnology. 2015;6(1):1-6.

Okoh AI. Assessement of the potentials of some bacterial isolates for application in the bioremediation of petroleum hydrocarbon polluted soil. Ph.D. thesis, Obafemi Awolowo University, Ile – Ife, Nigeria; 2002.

Okoh AI. Biodegradation of Bonny light crude oil in soil microcosm by some bacteria strains isolated from crude oil flow stations saver pits in Nigeria. African Journal Biotechnology. 2003;2(5):104-108.

Okpokwasili GC. Microbes and the Environmental Challenge.Inaugural Lecture Series No.53.University of Port Harcourt Press. Port Harcourt. 2006;31-36.

Olafisoye BO, Adefisoye T, Osibote OA. Heavy Metals Contamination of Water, Soil, and Plants around an Electronic Waste Dumpsite.Poland Journal of Environmental Studies. 2013;22(5):1431-1439.

Oldeman LR. Global extent of soil degradation.ISRIC Bi-Annual Report. Wagenngen. The Netherlands: ISRIC. 1992;19–36.

Onwurah INE. Restoring the crop sustaining potential of crude oil polluted soil by means of Azotobacter inoculation.Plant Product and Research Journal. 1999;4:6-16.

Onwurah INE, Nwuke EO. Crude oil spills in the environment, effects and some innovative clean-up biotechnologies. International Journal of Environmental Research. 2004;1(4):307-320.

Orji FA. Laboratory-Scale bioremediation of Crude – Oil polluted Mangrove Swamp in the Niger Delta using organic nutrient. M.Sc. thesis submitted to the School of Graduate Studies, University of Port Harcourt, Nigeria. 2011;154.

Osuji LC, Onojake I. An appraisal of the impact of petroleum hydrocarbons on soil fertility: the Owaza experience. Afr. J. Agric. Res. 2006;2(7):318-324.

Othman H, Mohammed AT, Dolmat MT. Bunch ash: an efficient and cost effective K fertilizer source for mature oil palm on peat under high rainfall environment, Malaysian Palm Oil Board. Inf. Ser. No. 2005;254.

Oviasogie PO, Aisueni NO, Brown GE. Oil palm composted biomass: A review of the preparation, utilization, handling and storage. African Journal of Agricultural Research. 2010;5(13):1553-1571.

Paerl H, Piehler M, Swistak J. Coastal diesel fuel pollution effects on the native microbial community. Poster presented at the Meeting of the American Society of Microbiology, New Orleans. 1996;19-23.

Papendick RI, Parr JF. Soil quality-the key to a sus¬tainable agriculture. American Journal of Alternative Agriculture. 1992;7:2-3.

Gervais G, Brosillon S, Laplanche A, Helen C. Ultra Pressure Liquid Chromatography-Electrospray Fandem Mass Spectrometry for Multi Residue Determination of Pesticides in Water. J. Chromatogr, A. 2008;1202(2):162-172.