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Background: Industries are the major sources of heavy metal pollution and it is released into water and soil. Heavy metals cause several negative effects to aquatic organisms and environment.
Aims: The present investigation aims to determine the effect of copper sulphate on protein content, free amino acids, AST and ALT levels in certain tissues, i.e. muscle and zliver of Mozambique tilapia, Oreochromis mossambicus.
Study Design: Oreochromis mossambicus weighing about 12- 14g used in the present study were procured from State fisheries culture tanks. They were transported to the laboratory in oxygenated containers and treated with KMnO4 to avoid dermal infection and acclimatized to laboratory conditions for 10 days.
Results and Discussion: In the present study, Oreochromis mossambicus was exposed to sublethal concentrations (1/16, 1/12, 1/8 and 1/4th of 96 h LC50 value) i.e. 3 mg/L, 4 mg/L, 6 mg/L and 12 mg/L of copper sulphate for four different exposure periods of 10, 20, 30 and 40 days. The proteins, free amino acids, Aspartate amino transferase (ASAT) and Alanine amine transferase (ALAT) levels in muscle and liver were studied. Decreased tendency was observed in protein levels and an increased tendency was observed in free amino acids, AST and ALT levels in all the vital tissues of fish exposed to copper sulphate over control.
Conclusion: Proteins gradually decreased with increased exposure period of sublethal concentrations. Amino acids, AST and ALT levels gradually increased with increased exposure period and the increase was observed to be directly proportional to the increased toxicant concentrations.