METABOLITES OF NATIVE LAB: AN ALTERNATIVE TO FISH SAFETY

Main Article Content

SUTAPA SANYAL

Abstract

The present paper delineated diverse perceptivity of Listeria monocytogenes (ATCC 657) and Aeromonas hydrophila (ATCC 646) to anti-listeria and anti-aeromonas compounds produced by different LAB (Lactic acid bacteria) like bacteria of fish. Fish intestine was searched for LAB in respect of their potential exploitation in fish biopreservation. Combative actions of ten LABs were assessed against the pathogenic and spoilage flora by agar well diffusion assay. LAB isolates were effectual in annihilation of indicator strains either with live cells and CFS (cell-free supernatant) or purely in the form of live cells. Antimicrobials were commenced to be functional against the both Gram positive and negative bacteria. Although varied modes of antagonism were reported.  The antimicrobial action of neutralised and catalase treated cell free supernatant fluid was secured against Listeria even after heating at 90°C for 10 min. Inhibition against Listeria was mostly exerted by thermostable products. No anti-aeromonas thermostable products were appeared to be linked with antagonism. Few thermolabile products were efficient against Aeromonas. Majority of LAB like isolates with thermostable anti-listeria products found to be worthwhile against Aeromonas with cells only. Proteinaceous antimicrobials competent against Aeromonas as well as Listeria retrieved from two isolates may be more efficacious in fish preservation than the usage of those bacteriocins that perform predominantly against Gram positive bacteria. The study appeals in situ application of indigenous natural bacteriocins of Oreochromis niloticus [Linnaeus, 1758] for shelf life lengthening of fish.

Keywords:
Antimicrobials, biopreservation, fish, LAB, proteinaceous, thermolabile, thermostable

Article Details

How to Cite
SANYAL, S. (2021). METABOLITES OF NATIVE LAB: AN ALTERNATIVE TO FISH SAFETY. UTTAR PRADESH JOURNAL OF ZOOLOGY, 42(7), 1-9. Retrieved from http://mbimph.com/index.php/UPJOZ/article/view/2031
Section
Original Research Article

References

Pilet MF, Leroi F. Applications of protective cultures, bacteriocins and bacteriophages in fresh seafood and seafood products. In Protective cultures, antimicrobial metabolites and bacteriophages for food and beverage biopreservation, Lacroix C, Zurich ETH (Eds.), Switzerland Woodhead Publishing Series in Food Science, Technology and Nutrition. 2011;201.

Mahmud A, Abraha B, Samuel M, Mohammedidris H, Abraham W, Mahmud E. Fish preservation: A multi-dimensional approach. MOJ Food Processing and Technology. 2018;6(3):303-310.

EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control). The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2011. EFSA Journal. 2013; 11(4)3129:1–250.

Jami M, Ghanbari M, Zunabovic M, Doming KJ, Kneifel W. Listeria monocytogenes in aquatic food products-a review, Comprehensive Reviews. Food Science and Food Safety. 2014;13:798–813.

Adams MR, Moss MO. Food Microbiology (second Ed.). Cambridge Royal Society of Chemistry; 2000.

Kirov SM. Aeromonas and Plesiomonas Species. In Doyle MP, Beuchat LR, Montville TJ (Eds.), Food Microbiology; 2001.

Fernandes CF, Flick GJ, Thomas TB. Growth of inoculated psychrotrophic pathogens on refrigerated fillets of aquacultured rainbow trout and channel catfish. Journal of Food Protection. 1998;61(3):313-317.

Giri SS, Sukumaran V, Sen SS, Vinumonia J. Antagonistic activity of cellular components of potential probiotic bacteria, isolated from the gut of Labeorohita, against Aeromonas hydrophila. Probiotics and Antimicrobial Proteins. 2011;3:214–222.

Zunabovic M, Domig K, Kneifel W. Practical relevance of methodologies for detecting and tracing of Listeria monocytogenes in ready-to-eat foods and manufacture environments - A review. LWT- Food Science Technology. 2011;44(2):351-362.

Bayles DO, Annous BA, Wilkinson BJ. Cold stress proteins induced in Listeria monocytogenes in response to temperature downshock and growth at low temperatures. Applied Environmental Microbiology. 1996; 62:1116-1119.

Messi P, Guerrieri E, Bondi M. Bacteriocin-like substance (BLS) production in Aeromonas hydrophila water isolates. FEMS Microbiology Letters. 2003;220:121–125.

Campos A, Castro P, Aubourg SP, Velázquez JB. Use of natural preservatives in seafood. In McElhatton AS (Eds.), Novel Technologies in Food Science, Integrating Food Science and Engineering Knowledge Into the Food Chain, Springer Science + Business Media. 2012;325-360.

Leroi F. Biopreservationof lightly preserved seafood products. INFOFISH International. 2011;4:41-46.

Alzamora S, Welti-Chanes J, Guerrero S. Rational use of novel technologies: A comparative analysis of the performance of several new food preservation technologies for microbial inactivation. In McElhatton AS(Eds.), Novel Technologies in Food Science, Integrating Food Science and Engineering Knowledge into the Food Chain, Springer Science + Business Media, LLC; 2012.

Schuppler M, Loessner MJ. The opportunistic pathogen Listeria monocytogenes: Pathogenicity and interaction with the mucosal immune system. International Journal of Inflammation. 2010;1-12.

Yamazaki K, Suzuki M, Kawai Y, Inoue N, Montville TJ. Inhibition of Listeria monocytogenes in cold-smoked salmon by Carnobacterium piscicola CS526 isolated from frozen surimi. Journal of Food Protection. 2003;66:1420-1425.

Campos CA, Rodriguez O, Mata CP, Prado M, Velazquez BJ. Preliminary characterization of bacteriocins from Lactococcus lactis, Enterococcus faecium and Enterococcus mundtii strains isolated from turbot (Psetta maxima). Food Research International. 2006; 39:356–364.

Tome E, Teixeira P, Gibbs PA. Anti-listerial inhibitory lactic acid bacteria isolated from commercial cold smoked salmon. Food Microbiology. 2006;23:399-405.

Calo-Mata P, Arlindo S, Boehme K, Miguel TD, Pascoal A, Barros-Vel_azquez J. Current applications and future trends of lactic acid bacteria and their bacteriocins for the biopreservation of aquatic food products. Food Bioprocess Technology. 2008;1:43-63.

Brillet A, Pilet MF, Prevost H, Bouttefroy A, Leroi F. Biodiversity of Listeria monocytogenes sensitivity to bacteriocin-producing Carnobacterium strains and application in sterile cold-smoked salmon. Journal of Applied Microbiology. 2004;97: 1029-1037.

Brillet A, Pilet MF, Prevost H, Cardinal M, Leroi F. Effect of inoculation of Carnobacterium divergens, a biopreservative strain against Listeria monocytogenes risk, on the microbiological, and sensory quality of cold-smoked salmon. International Journal of Food Microbiology. 2005;41(104): 309-324.

Kwaadsteniet M, Doeschate KT, Dicks LMT. Characterization of the structural gene encoding nisin F, a new lantibiotic produced by a Lactococcus lactis subsp. lactis isolate from freshwater catfish (Clarias gariepinus). Applied Environmental Biology. 2008;74:547–549.

Rumjuankiat K, Pilasombut K, Wangwibulkit S, Swetwiwathana A. Screening and partial characterization of bacteriocin from lactic acid bacteria in fish gastrointestinal tract. Khon Kaen University Research Journal. 2010;15 (9):870-877.

Sahoo TK, Jena PK, Patel AK, Seshadri S. Purification and Molecular Characterization of the Novel Highly Potent Bacteriocin TSU4 Produced by Lactobacillus animalis TSU4. Appl Biochemistry and Biotechnology. 2015; 177(1):90-104.

Indira K, Jayalakshmi S, Gopalakrishnan A, Srinivasan M. Biopreservative potential of marine Lactobacillus spp. India. African Journal of Microbiology Research. 2011;5(16): 2287-2296.

Banerjee SP, Dora KC, Chowdhury S. Detection, partial purification and characterization of bacteriocin produced by Lactobacillus brevis FPTLB3 isolated from freshwater fish Bacteriocin from Lb. brevis FPTLB3. Journal of Food Science and Technology. 2011;50 (1):17-25.

Vijayabaskar P, Somasundaram ST. Isolation of bacteriocin producing lactic acid bacteria from fish gut and probiotic activity against common freshwater fish pathogen Aeromonas hydrophila. Biotechnology. 2008;7(1):124-128.

Nilsson L, Huss HH, Gram L. Inhibition of Listeria monocytogenes on cold-smoked salmon by nisin and carbon dioxide atmosphere. International Journal of Food Microbiology. 1997;38(2-3):217-227.

Einarsson H, Lauzon HL. Biopreservation of brined shrimp (Pandalus borealis) by bacteriocins from lactic acid bacteria. Applied Environmental Microbiology. 1995;61(2):669-676.

Zuckerman H, Avraham BR. Control of growth of L.monocytogenes in fresh salmon using Microgard (TM) and Nisin. Lebensmittel-Wissenschaft Und-Technologie. 2002;35(6): 543-548.

Nykänen A, Lapveteläinen A, Heitanen RM, Kallio H. Synergistic inhibition of Listeria monocytogenes on cold-smoked rainbow trout by nisin and sodium lactate. Lebensmittel-Wissenschaft und Technologies. 1989;35:543-548.

Niskänen A, Nurmi E. Effect of starter culture on staphylococcal enterotoxin and thermonuclease production in dry sausage. Applied Environmental Microbiology. 2000; 31:11–20.

Sudalayandi KM, Manja. Efficacy of lactic acid bacteria in the reduction of trimethylamine-nitrogen and related spoilage derivatives of fresh Indian mackerel fish chunks. African Journal of Biotechnology. 2011;10(1):42-47.

Sarika AR, Lipton AP, Aishwarya MS, Dhivya RS. Efficacy of Bacteriocin of Enterococcus faecalis CD1 as a Biopreservative for high value marine fish reef cod (Epinephelus diacanthus) under different storage conditions. Journal of Microbiology and Biotechnology Research. 2011;1(4):18-24

Uddin N, Al-Harbi AH. Bacterial flora of polycultured common carp (Cyprinus carpio) and African catfish (Clariasgariepinus). International Aquatic Research. 2012;4(10): 2-9.

Sahnouni F, Boutiba-Maatallah A, Bouhadi D, Boutiba Z. Characterization of bacteriocin produced by Lactococcus lactis ssp. lactis strains isolatedfrom marine fish caught in the Algerian west coast. Turkish Journal of Agricultural and Natural Sciences. 2014; 2(2014):1838-1843.

Cappuccino G, Sherman N. Microbiology a laboratory manual. Benjamin Cumming (Ed.), New York. 2002;1.

Balcazar JL, Vendrell D, de Blas I. Ruiz-Zarzuela I, Muzquiz JL, Girones O. Characterization of probiotic properties of lactic acid bacteria isolated from intestinal microbiota of fish. Aquaculture. 2008;278:188-191.

Složilová I, Purkrtová S, Kosová M, Mihulová M, Šviráková E, Demnerová K. Antilisterial activity of lactic acid bacteria against Listeria monocytogenes strains originating from different sources. Czech Journal of Food Science. 2014;32(2):145–151.

Jini R, Swapna HC, Rai AK, Vrinda R, Halami PM, Sachindra NM, et al. Isolation and characterization of potential lactic acid bacteria (lab) from freshwater fish processing wastes for application in fermentative utilisation of fish processing waste. Brazilian Journal of Microbiology. 2011;42:1516-1525.

Schelegueda LI, Vallejo M, Gliemmo MF, Marguet ER, Campos CA. Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis. LWT- Food Science and Technology. 2015;64(2015):794-801.

Udhayashree N, Senbagam D, Senthilkumar B, Nithya KJ, Gurusamy R. Production of bacteriocin and their application in food products. Asian Pacific Journal of Tropical Biomedicine. 2012;2(1):406-410.

Rammelsberg M, Radler F. Antibacterial polypeptides of Lactobacillus species. Journal of Applied Bacteriology. 1990;69:177-184.

Boutin SB, Bernatchez L, Audet CL, Nicolas DM. Antagonistic effect of indigenous skin bacteria of brook charr (Salvelinus fontinalis) against Flavobacterium columnare and F. psychrophilum. Veterinary Microbiology. 2012;155:355–361.

Deegan LH, Cotter PD, Hill C, Ross P. Bacteriocins: Biological tools for biopreservation and shelf-life extension. International Dairy Journal. 2006;16:1058-1071.

Mortvedt-Abildgaa CI, Nissen-Meyer J, Jelle B, Grenov B, Skaugen M, Nes IF. Production and pH-dependent bactericidal activity of lactocin s, a lantibiotic from Lactobacillus sake L45. Applied and Environmental Microbiology. 1995;61(1):175-179.

Liu W, Hansen JN. Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lactis. Applied and Environmental Microbiology. 1990;56:2551–2558.

Jennes W, Dicks LMT, Verwoerd DJ. Enterocin 012, a bacteriocin produced by Enterococcus gallinarum isolated from the intestinal tract of ostrich. Journal of Applied Microbiology. 2000;88(2):349-57.

Drider D, Fimland G, Héchard Y, McMullen LM, Prévos H. The continuing story of class IIa bacteriocins. Microbiology and Molecular Biology Reviews. 2006;70:564– 582.

Hartmann HA, Wilke T, Erdmann R. Efficacy of bacteriocin-containing cell-free culture supernatants from lactic acid bacteria to control Listeria monocytogenes in food. International. Journal of Food Microbiology. 2011;146:192-199.

Gálvez A, Abriouel H, Lucas López R, Ben Omar N. Bacteriocin-based strategies for food biopreservation. International Journal of Food Microbiology. 2007;120:51–70.

Aasen IM, Markussen S, Moretro T, Katla T, Axelsson L, Naterstad L. Interactions of the bacteriocin sakacin P and nisin with food constituents. International Journal of Food Microbiology. 2003;87:35–43.

Campos CA, Castro MP, Rivas FP, Schelegueda LI. Bacteriocins in food: Evaluation of the factors affecting their effectiveness. Microbial Pathogens and Strategies for Combating them: Science, Technology and Education, (A. Méndez-Vilas, Ed.). 2013;994-1004.

Yang SC, Hunglin C, Sung CT, Youfang J. Antibacterial activities of bacteriocins: Application in foods and pharmaceuticals. Frontiers in Microbiology. 2014;5:1-10.