ANTAGONISTIC ACTIVITY OF GUT ASSOCIATED PROBIOTIC BACTERIA ISOLATED FROM Etroplus maculatus (BLOCH, 1795)

Main Article Content

S. MUTHUKRISHNAN
P. RAJA
J. RONALD

Abstract

Probiotics are living microorganisms that have positive effects on the health of the digestive system when acquired in a defined dosage and helps to stimulate the immune response in fish against deadly pathogens. Therefore, focusing on the maintenance of gastrointestinal health is more essential. This study aimed to determine the antagonistic activity of bacteria isolated from the gut of freshwater fish Etroplus maculatus. In the present study, we isolated 20 bacterial isolates with different colony morphology; further screened for antibacterial activity using the cross streaking and disk diffusion method against Aeromonas hydrophila, Pseudomonas aeruginosa, Vibrio cholerae, and Staphylococcus aureus. In the preliminary screening isolates EF1 and EM9 were found to have prominent antagonistic activity. In the cross-streaking method, the isolates EM9 showed the highest level of inhibitory (85%) against Staphylococcus aureus; a moderate level of activity in EM9 against Aeromonas hydrophila and the lowest level of inhibition in EF1 and EM9 against Pseudomonas aeruginosa and Vibrio cholerae. The bacterial metabolites of active bacterial isolates (EF1 and EM9) were screened for their bioactive potential. Ethyl acetate extract of EM9 isolate has been shown to have predominant activity against fish pathogens at 100 µg/disc concentration with a zone of inhibition of 14mm against Staphylococcus aureus followed by EF1 where the zone of inhibition was highest with 12mm. The active bacterial isolates were identified as Escherichia sp and Bacillus sp based on morphological and biochemical characteristics. This investigation clearly shows that the fish gut can be explored as a source of antagonistic bacteria which produces antimicrobial compounds of wide spectrum of bioactivity. These bacterial secondary metabolites have warranted much research interest because of their biochemical diversity, broad specificity on antimicrobial, and even anti-tumoural or wound-healing effects.

Keywords:
Etroplus maculatus, gut probiotics, antagonistic activity

Article Details

How to Cite
MUTHUKRISHNAN, S., RAJA, P., & RONALD, J. (2021). ANTAGONISTIC ACTIVITY OF GUT ASSOCIATED PROBIOTIC BACTERIA ISOLATED FROM Etroplus maculatus (BLOCH, 1795). UTTAR PRADESH JOURNAL OF ZOOLOGY, 42(24), 138-147. Retrieved from http://mbimph.com/index.php/UPJOZ/article/view/2669
Section
Original Research Article

References

NABARD P. National Bank for Agriculture and Rural Development. Mumbai. 2008-2018.

Bondad-Reantaso MG, Subasinghe RP, Arthur JR, Ogawa K, Chinabut S, Adlard R, Tan Z, Shariff M. Disease and health management in Asian aquaculture. Veterinary Parasitology. 2005;132(3-4):249-72.

FAO/WHO, Guidelines for the Evaluation of Probiotics in Food. 2006.

Nomoto K. Prevention of infections by probiotics. Journal of Bioscience and Bioengineering. 2005;100(6):583-92.

Kozasa M. Toyocerin (Bacillus toyoi) as growth promotor for animal feeding. Microbiol. Aliment. Nutrition. 1986;4:121-35.

Moriarty DJW. Control of luminous Vibrio species in penaeid aquaculture ponds. Aquaculture. 1998;164(1-4):351-358.

Wang YB, Li JR, Lin J. Probiotics in aquaculture: challenges and outlook. Aquaculture. 2008 Sep 1;281(1-4):1-4.

Irianto A, Austin B. Probiotics in aquaculture. Journal of Fish Diseases. 2002;25(11):633-42.

Hibbing ME, Fuqua C, Parsek MR, Peterson SB. Bacterial competition: surviving and thriving in the microbial jungle. Nature reviews Microbiology. 2010;8(1):15-25.

Demain AL, Sanchez S. Microbial drug discovery: 80 years of progress. The Journal of antibiotics. 2009;62(1):5-16.

Debbab A, Aly AH, Lin WH, Proksch P. Bioactive compounds from marine bacteria and fungi. Microbial Biotechnology. 2010;3(5):544-63.

Silphaduang U, Noga EJ. Peptide antibiotics in mast cells of fish. Nature. 2001;414(6861):268-9.

Wang AR, Ran C, Ringo E, Zhou ZG. Progress in fish gastrointestinal microbiota research. Reviews in Aquaculture. 2018;10(3):626-40.

Ruiz B, Chavez A, Forero A, Garcia-Huante Y, Romero A, Sanchez M, Rocha D, Sánchez B, Rodríguez-Sanoja R, Sanchez S, Langley E. Production of microbial secondary metabolites: regulation by the carbon source. Critical Reviews in microbiology. 2010: 36(2):146-167.

Jayaram KC. The Freshwater Fishes of the Indian Region. Narendra Publishing House, Delhi, India, 1999;551.

Spanggaard B, Huber I, Nielsen J, Nielsen T, Appel KF, Gram L. The microflora of rainbow trout intestine: a comparison of traditional and molecular identification. Aquaculture, 2000:182 (1):1-15.

Ellaiah P, Kalyan D, Rao VS, Rao BV. A new variety of Chainia olivacea from marine sediment off Gulf of Mannar. Hindustan Antibiotics Bulletin. 1997;39(1-4):50-5.

Balagurunathan R. PhD thesis. Annamalai University (Paragipettai, India). 1992.

Spragg MA, Boyd KG, Hubble MO, Burgess JG. Antibiotics from surface-associated marine bacteria Proceedings of the Fourth under water science symposium. Society for Under Water Technology, London, ISBN. 1997;906940311:147-57.

Naikpatil SV, Rathod JL. Selective isolation and antimicrobial activity of rare actinomycetes from mangrove sediment of Karwar. Journal of Ecobiotechnology. 2011.

Samelis J, Maurogenakis F, Metaxopoulos J. Characterisation of lactic acid bacteria isolated from naturally fermented Greek dry salami. International Journal of Food Microbiology. 1994;23(2):179-96.

Balcázar JL, Vendrell D, de Blas I, Ruiz-Zarzuela I, Muzquiz JL, Girones O. Characterization of probiotic properties of lactic acid bacteria isolated from intestinal microbiota of fish. Aquaculture. 2008;278(1-4):188-91.

Kim DH, Austin B. Characterization of probiotic carnobacteria isolated from rainbow trout (Oncorhynchus mykiss) intestine. Letters in applied microbiology. 2008;47(3):141-7.

Allameh SK, Daud H, Yusoff FM, Saad CR, Ideris A. Isolation, identification and characterization of Leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (Channa striatus). African Journal of Biotechnology. 2012;11(16): 3810-6.

Fjellheim AJ, Klinkenberg G, Skjermo J, Aasen IM, Vadstein O. Selection of candidate probionts by two different screening strategies from Atlantic cod (Gadus morhua L.) larvae. Veterinary Microbiology. 2010;144(1-2):153-9.

Vijayabaskar P, Somasundaram ST. Isolation of bacteriocin producing lactic acid bacteria from fish gut and probiotic activity against common fresh water fish pathogen Aeromonas hydrophila. Biotechnology. 2008;7(1): 124-8.

Henriques AO, Moran Jr CP. Structure and assembly of the bacterial endospore coat. Methods. 2000;20(1):95-110.

Nicholson WL, Munakata N, Horneck G, Melosh HJ, Setlow P. Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiology and Molecular Biology Reviews. 2000;64(3):548-72.

Vendrell D, Balcázar JL, de Blas I, Ruiz-Zarzuela I, Gironés O, Muzquiz JL. Protection of rainbow trout (Oncorhynchus mykiss) from lactococcosis by probiotic bacteria. Comparative Immunology, Microbiology and Infectious Diseases. 2008;31(4):337-45.

Merrifield DL, Dimitroglou A, Foey A, Davies SJ, Baker RT, Bogwald J, Castex M, Ringo E. The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture. 2010;302(1-2):1-8.

Son VM, Chang CC, Wu MC, Guu YK, Chiu CH, Cheng W. Dietary administration of the probiotic, Lactobacillus plantarum, enhanced the growth, innate immune responses, and disease resistance of the grouper Epinephelus coioides. Fish & Shellfish Immunology. 2009;26(5):691-8.

Asla V, Neethu KP, Athira V, Nashad M, Mohamed HA. Prevalence of Antibiotic Resistance among the Gut Associated Bacteria of Indigenous Freshwater Fishes Aplocheilus lineatus and Etroplus maculatus. International Journal of Aquaculture. 2016;12: 6.

Salminen S, Von Wright A, editors. Lactic acid bacteria: microbiological and functional aspects. CRC Press;2004;23:139.

Cebeci A, Gürakan C. Properties of potential probiotic Lactobacillus plantarum strains. Food microbiology. 2003;20(5):511-8.