ISOLATION OF Staphylococcus warneri FROM THE GUT OF Armigeres subalbatus USING 16S RIBOSOMAL RNA SEQUENCE

Main Article Content



Mosquitoes are medically significant as vectors of many epidemics such as malaria, dengue fever and filariasis. The gut of mosquito is inhabited by various bacterial flora and they assist in many physiological functions including its pathogen transmitting ability. The disease transmitting efficiency of the mosquito can be reduced by making transgenic variation in gut bacteria. Proper identification of gut bacterial flora is needed to select an ideal bacterial species for transgenic purpose. In the present study the haemolytic gut bacteria Staphylococcus warneri is isolated from the adult female Armigeres subalbatus and identified by using 16S rRNA sequencing.

Haemolytic gut bacteria, 16S rRNA sequencing, Armigeres subalbatus, Staphylococcus warneri

Article Details

How to Cite
JITHINA, K. C., BABU, A., JAYAKRISHNAN, T. V., RAGHUNATHAN, P. P., & JOSEPH, V. J. (2021). ISOLATION OF Staphylococcus warneri FROM THE GUT OF Armigeres subalbatus USING 16S RIBOSOMAL RNA SEQUENCE. UTTAR PRADESH JOURNAL OF ZOOLOGY, 42(24), 237-242. Retrieved from
Short Research Article


Lindh JM, Terenius O, Faye I. 16S rRNA gene-based identification of midgut bacteria from field-caught Anopheles gambiae sensu lato and A. funestus mosquitoes reveals new species related to known insect symbionts. Applied and Environmental Microbiology. 2005;71(11): 7217-23.

Rani A, Sharma A, Rajagopal R, Adak T, Bhatnagar RK. Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi-an Asian malarial vector. BMC microbiology. 2009;9(1):1-22.

Gunathilaka N, Ranasinghe K, Amarasinghe D, Rodrigo W, Mallawarachchi H, Chandrasena N. Molecular Characterization of Culturable Aerobic Bacteria in the Midgut of Field-Caught Culex tritaeniorhynchus, Culex gelidus, and Mansonia annulifera Mosquitoes in the Gampaha District of Sri Lanka. BioMed Research International; 2020.

Ferguson MJ, Micks DW. Microorganisms associated with Mosquitoes. I. Bacteria isolated from the Mid-Gut of Adult Culex fatigans Wiedemann. Journal of Insect Pathology. 1961; 3(2):112-9.

Yadav KK, Datta S, Naglot A, Bora A, Hmuaka V, Bhagyawant S, Gogoi HK, Veer V, Raju PS. Diversity of cultivable midgut microbiota at different stages of the Asian tiger mosquito, Aedes albopictus from Tezpur, India. PLoS One. 2016;11(12): e0167409.

Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press; 1989.

Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology. 1991;173(2):697-703.

Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences. 1977;74(12):5463-7.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. Journal of molecular biology. 1990 Oct 5;215(3):403-10.

Tamura K, Stecher G, Kumar S. MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution. 2021;38(7):3022-7.

Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution. 1980;16(2): 111-20.

Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39(4):783-91.

Reinert JF, Harbach RE, Kitching IJ. Phylogeny and classification of tribe Aedini (Diptera: Culicidae). Zoological Journal of the Linnean Society. 2009;157(4):700-94.

Finney CA, Kamhawi S, Wasmuth JD. Does the arthropod microbiota impact the establishment of vector-borne diseases in mammalian hosts. PLoS Pathogens. 2015;11(4):e1004646.

Cirimotich CM, Ramirez JL, Dimopoulos G. Native microbiota shape insect vector competence for human pathogens. Cell Host & Microbe. 2011;10(4):307-10.

Chouaia B, Rossi P, Montagna M, Ricci I, Crotti E, Damiani C, Epis S, Faye I, Sagnon NF, Alma A, Favia G. Molecular evidence for multiple infections as revealed by typing of Asaia bacterial symbionts of four mosquito species. Applied and Environmental Microbiology. 2010;76(22):7444-50.

Buck M, Nilsson LK, Brunius C, Dabiré RK, Hopkins R, Terenius O. Bacterial associations reveal spatial population dynamics in Anopheles gambiae mosquitoes. Scientific Reports. 2016;6(1):1-9.

Minard G, Mavingui P, Moro CV. Diversity and function of bacterial microbiota in the mosquito holobiont. Parasites & Vectors. 2013;6(1):1-2.

Moro CV, Tran FH, Raharimalala FN, Ravelonandro P, Mavingui P. Diversity of culturable bacteria including Pantoea in wild mosquito Aedes albopictus. BMC Microbiology. 2013;13(1):1-1.

De Oliveira Gaio A, Rodrigues RC, do Nascimento C, Secundino NF, Lemos FJ, Pimenta PF, Monesi N. Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera: Culicidae) (L.). Parasites & Vectors. 2011;4(1):1-5.