PHYTOCHEMICAL SCREENING AND COMPARATIVE GC–MS ANALYSIS OF BIOACTIVE COMPOUNDS PRESENT IN METHANOLIC LEAF AND LATEX EXTRACT Calotropis gigantea (L)

Main Article Content

S. AZHAGU MADHAVAN
P. VINOTHA
V. UMA

Abstract

Medicinal plants are considered as important source of promising bioactive compounds. Calotropis gigantea is a traditional medicinal plant which is known to have biochemical constituents with potential medicinal properties. Qualitative analysis showed the presence of alkaloids, terpenoids, saponins, tannins and cardiac glycosides and absence of flavonoids in ethanolic extract of C. gigantea, while the chloroform leaf extract showed absence of flavonoids and cardiac glycosides. Bioactive compounds since leaf and latex of C. gigantea utilized GC-MS and activity investigation. The GC-MS investigation uncovered the presence of complete 46 bioactive mixes (24 from leaves and 22 from latex) with significant movement. The majority of the mixes were discovered to be comparable in both leaf and latex, however little variety was likewise seen in their synthetic profile. The concoction mixes saw in just latex were 1-[(T-butyl) dimethyl silyl thin] butane, 1-Hexadecyne, Hexadecane, L-Glutamic corrosive, Phenol-3-isoproxy-5-methyl, Trocosane and Z-1,6-Tridecadiene. Mixes distinguished uniquely from the leaves were Azulene, Benalaxyl, Cis-vaccenic corrosive, Levomenol, Profenofos, β-Tocopherol and β-Sitosterol, while the remainder of the mixes were comparable in both leaf and latex. Major of bioactive compounds presents Gas Chromatography-Mass Spectroscopy (GC-MS) analysis. C. gigantea is a perennial plant abundantly found in all parts of the country (India) and wild in nature. The leaves of the plant were found to contain various primary and secondary metabolites.

Keywords:
Β-Tocopherol, Calotropis gigantean, GC-MS, phytochemical, phlobatannins.

Article Details

How to Cite
MADHAVAN, S. A., VINOTHA, P., & UMA, V. (2020). PHYTOCHEMICAL SCREENING AND COMPARATIVE GC–MS ANALYSIS OF BIOACTIVE COMPOUNDS PRESENT IN METHANOLIC LEAF AND LATEX EXTRACT Calotropis gigantea (L). Asian Journal of Advances in Medical Science, 2(2), 1-13. Retrieved from https://mbimph.com/index.php/AJOAIMS/article/view/1679
Section
Original Research Article

References

Surabhi Bajpai, Himani Hooda, Singh RK, Rakesh Mishra. Comparative analysis of antioxidant properties of extracts of Calotropis procera with different anti-diabetic drugs. International Journal of Herbal Medicine. 2018;6(2):104-109.

Chandrawat P, Sharma RA. GC-MS analysis of fruits of Calotropis procera: A medicinal shrub. Research Journal of Recent Sciences. 2015;4(IVC-2015);11-14. ISSN: 2277-2502.

Sonia Angeline M, Sonali Bhattacharjee, Kikim Lotjem. Anti-proliferative activity of Calotropis gigantea (Flower Extract) on colorectal cancer cell line. Int J Life Sci Pharma Res. 2020;SP-11. Inclusive Development through Biotechnology; 2020. ISSN: 2250 – 0480.

Waseem Ahmada. Current chemistry letters 9 a organic chemistry section, Department of Chemistry, Uttaranchal University, Dehradun 248001, India; 2020.

Manivannan R, Shopna R. Antidiabetic activity of gigantea white flower extracts in alloxan induced diabetic rats. Journal of Drug Delivery and Therapeutics. 2017;7(3):106-111.
DOI: http://dx.doi.org/10.22270/jddt.v7i3.1447

Rajendra Gyawali, Bijay Bhattarai, Susan Bajracharya, Surakshya Bhandari, Puja Bhetwal, Kanchan Bogati, Sahana Shrestha, Ashwinee Kumar Shrestha, Shardul Neupane, Rajendra Joshi, Prem Narayan Paudel. α-amylase inhibition, antioxidant activity and phytochemical analysis of Calotropis gigantea (L.) Dryand. JHAS. 2020;10(1):2020.
Available:www.jhas.org.np
DOI: https://doi.org/10.37107/jhas.143

Damodaran B, Nagaraja P, Jain V, Manuja Viraj Wimalasiri MP, Sankolli GM, Kumar GV, et al. Phytochemical screening and evaluation of cytotoxic activity of Calotropis gigantea leaf extract on MCF7, HeLa and A549 cancer cell lines. J Nat Sc Biol Med. 2019;10:131-8.

Amjad Ibraim Oraibi, Maha N. Hamad. Phytochemical investigation of flavanoid of Calotropis Procera in Iraq, Isolation and identification of rutin, quercitin and kampferol. J. Pharm. Sci. & Res. 2018;10(9):2407-2411.

Sri Padmapriya R, Anita R, Singh J. Milkweed plant calotropis. A review. 2018;2–6.

Deepika Srivastava, Padma Singh. In – vitro fungitoxic evaluation and GCMS analysis of Calotropis procera. World Journal of Pharmaceutical Research. 2015;4:3.

Mohamed AA, Ali SI, Sameeh MY, Abd El-Razik TM. Effect of solvents extraction on HPLC profile of phenolic compounds, antioxidant and anticoagulant properties of Origanum vulgare. Research J. Pharm. and Tech. 2016;9(11):2009-2016.

Manal Y. Sameeh, Amal A. Mohamed. Characterization of polyphenols by HPLC, their antioxidant and GC-MS analysis of wild Calotropis procera leaves and fruit extracts. International Journal of ChemTech Research. 2018;11(01):319-327.

Sachin S, Asha Rani, Nagarathna Amresh, Murugan Rajadurai, Balasubramanian Sathyamurthy. Phytochemical studies on the methanolic extract of Calotropis gigantea leaves. IAJPS. 2018;05(07):6248-6260.
Available:http://doi.org/10.5281/zenodo.1307382

Aicha Blama Merzaia, Huma Riaz, Rafia Rehman, Shafaq Nisar, Muhammad Waqar Azeem. A review of toxicity, therapeutic and biological activities of Calotropis. IJCBS. 112017:58-64.

Estabraq H. Naser, Abbas M. Kashmer, Salam A. Abed. Antibacterial activity and phytochemical investigation of leaves of Calotropis procera plant in Iraq by GC-MS. IJPSR. 2019;10(4):1988-1994.

Prathiba R, Shruth Mi, Miranda LR. Pyrolysis of polystyrene waste in the presence of activated carbon in conventional and microwave heating using modified thermo-couple. Waste Management. 2018;76:528-536.

Radhaboya G, Pugazhvadivu M. Properties of bio-oil produced by co-pyrolysis of Procera stem and waste Polystyrene National Conference on Energy and Chemicals from Biomass (NCECB) AIP Conf. Proc. 2225, 040003-1–040003-5; 2020.
Available:https://doi.org/10.1063/5.0006401 Published by AIP Publishing.978-0-7354-1984-1/$30.00

Sunita Arora, Sonam Meena. GC-MS profiling of Ceropegia bulbosa Roxb. var. bulbosa, an endangered plant from Thar Desert, Rajasthan. The Pharma Innovation Journal. 2017;6(11):568-573.

Ihsan Ullah, Khalid MS. Al-Ghamdi, Yasir Anwar, Jazem A. Mahyoub. Exploring the insect control and plant growth promotion potentials of endophytes isolated from Calotropis procera present in Jeddah KSA. Natural Product Communications. 2020;15(3):1–9. © The author(s) article reuse guidelines: sagepub.com/ journals-permissions.
DOI: 10.1177/1934578X20912869

Khandelwal KR. Practical pharmacognosy techniques and experiments. New Delhi: Nirali Prakashan. 2002;15-163.

Kokate CK. Practical pharmacognosy. 1st Ed. New Delhi: Vallabh Prakashan. 2005;111.

British Pharmacopoeia. 1st Edition, TSO Publisher, London, United Kingdom. 2020;5000.

Andrews S, Azhagu Madhavan S, Ganesan S, Arjun P, Jeyaprakash R, Baskara Sanjeevi S, Ramasamy M. Different bioactive constituents and biochemical composition of brown seaweed Spatoglossum marginatum. Waffen-Und Kostumkunde Journal. 2020;XI(IV). ISSN: 0042-9945.

Mahadevi M, Azhagu Madhavan S. Pharmacognostical and phytochemical screening of physico-chemical parameters and fluorescence analysis on ethanolic leaves extract of Ipomoea sepiaria KOENIG EX. ROXB. Waffen-Und Kostumkunde Journal. 2020;XI(VIII). ISSN: 0042-9945.

Azhagu Madhavan S, Vinotha P, Uma V, Mahadevi M. Anticancer activity of Pedalium murex linn methanolic leaves extract against A549 human lung cancer cell line. Asian Journal of Advances in Research. 2020;5(1): 33-40.

Asoso Oluwakemi S, Oladunmoye MK, Ogundare. Antibacterial screening of crude fractions of Calotropis procera (Linn) and GC-MS profile of fractions obtained from the extracts of Calotropis procera. Biomed J Sci & Tech Res. 2019;18(2). BJSTR. MS.ID.003124.

Sureshkumar P, Suresh E. Seasonal discrepancies of some terpenes in the milkweed Calotropis gigantea (L.) R. Br. IJRAR. 2018;5(4).

Shopna Rajamohan, Prabakaran Kalaivanan, Ilayaraja Sivagnanam, Manivannan Rajamanickam. Antioxidant, antimicrobial activities and GC-MS analysis of Calotropis gigantea white flowers. The Journal of Phytopharmacology. 2014;3(6):405-409.

Shalini Sharma, Amita Kumari, Mamta Sharma. Comparative GC-MS analysis of bioactive compounds in methanolic extract of Calotropis gigantea (L) W.T. Aiton leaf and latex. International Journal of Pharmacognosy and Phytochemical Research. 2016;8(11):1823-1827.