Published: 2021-09-02

Page: 220-224


Department of Biotechnology, Bishop Heber College, Trichy, India.


Department of Biotechnology, Bishop Heber College, Trichy, India.


Department of Zoology, Bishop Heber College, Trichy, India.

*Author to whom correspondence should be addressed.


This study aimed to evaluate the cytotoxicity of Elephantopus scaber leaves against breast cancer cell lines. Leaves of the plant were collected, washed, shade dried and powdered. The ethanol extract of the leaf powder was used for the study. The anticancer activity of the ethanol extract of the leaves was evaluated using MTT assay against MCF-7 cells and was confirmed by ETBR-AO staining. The study confirms that the leaf extract of  E.scaber has pronounced anticancer potential against MCF-7 cell lines. The plant investigated possesses remarkable anticancer activity, where the effect was dose dependent and the IC50 value was found to be 79.56 μg /ml. Since the preliminary data is promising, isolation of the compound may contribute to the development  at a novel and natural phytomedicine for the disease. Additional studies are needed to determine its mode of action.

Keywords: Elephantopus scaber, MTT assay, anti-cancer, MCF-7, ETBR-AO stain, ethanol extract

How to Cite

JASMINE, R., ABARNA, N., & VERGHESE, S. (2021). ANTICANCER POTENTIAL OF Elephantopus scaber L. LEAVES AGAINST MCF -7 CELL LINES. Asian Journal of Advances in Medical Science, 3(1), 220–224. Retrieved from


Download data is not yet available.


WHO fact sheet. World Health Organization Committee. 2017;186.

Koda Kimble LYY, Wayne A, Kradjan BJG, Brain KA, Robin LC.2002. Applied therapeutics the clinical use of drugs. In: Troy D, editor. Handbook of Applied therapeutics. Philadelphia: Lippincott Williams & Wilkins.

Greenwell M, Rahman PKSM. Medicinal Plants: Their use in anticancer Treatment. International Journal of Pharmaceutical Sciences and Research. 2015;6:4103-4112.

Costa-Lotufo LV, Khan MTH, Ather A, Wilke DV, Jimenez PC, Pessoa C, Amaral de Moraes ME, Odorico de Moraes M. Studies of the anticancer potential of plants used in Bangladeshi folk medicine. Journal of Ethnopharmacology. 2005;99:21–30.

Ho WY, Ky H, Yeap SK, Rahim RA, Omar AR, Ho CL. Traditional practices of bioactivities and commercialization potential of Elephantopus scaber Linn. J Med Plants Res. 2009;3(13):1212–1221.

Inta A, Shengji P, Balslev H, Wangpakapattanawong P, Trisonthi C. A comparative study on medicinal plants used in Akha’s traditional medicine in China and Thailand, cultural coherence or ecological divergence? J. Ethnopharmacol. 2008;116:508-517.

Yam MF, Lee FA, Omar ZA, Ibrahim MS, Hesham A, Mohd ZA. Anti-inflammatory and analgesic effects of Elephantopus tomentosus ethanolic extract. J Acupunct Meridian Stud. 2009;2(4):280−287.

Achuta N, Shukla SS, Rawat AKS. An ethnobotanical study of medicinal plants of Rewa district Madhya Pradesh. Indian Journal of Traditional Knowledge. 2010;9(1):191-202.

Jasmine R, Daisy P, Selvakumar BN. A novel terpenoid from Elephantopus scaber with Antibacterial activity against beta lactamase- producing clinical isolates. Res. J. of Microbiol. 2007;2(10):770-775.

Daniel M. Medicinal plants: chemistry and properties. Science Publishers Enfield, New Hampshire. 2006; 03748: 85-86.

Julius K. An ethnobotanical survey of medicinal and other useful plants of Muruts in Sabah, Malaysia. Ethnobotany Section, Forest Research Centre, Forestry Department Sabah, P.O. Box 1407, 90715 Sandakan, Sabah, Malaysia. Telopea. 2003;10(1):81–98.

Daisy P, R.Jasmine,S, Ignacimuthu, E.Murugan, 2009. A novel Steroid from Elephantopus scaber L. an Ethnomedicinal plant with antidiabetic activity, Phytomedicine , 16, 2–3: 252-257.

Wu XT, Liu JQ, Lu XT, Chen FX, Zhou ZH, Wang T, Zhu SP, Fei SJ. The enhanced effect of lupeol on the destruction of gastric cancer cells by NK cells. Int Immunopharmacol. 2013;16(2):332-340.

Chan CK, Supriady H, Goh BH, Kadir HA. Elephantopus scaber induces apoptosis through ROS-dependent mitochondrial signaling pathway in HCT116 human colorectal carcinoma cells. J Ethnopharmacol. 2015;168:291-304.

Pitchai D, Roy A, Ignatius C. In vitro evaluation of anticancer potentials of lupeol isolated from Elephantopus scaber L. on MCF-7 cell line. J Adv Pharm Technol Res. 2014;5(4):179-184.