A STUDY ON DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF CEFPODOXIME IN BULK AND A PHARMACEUTICAL DOSAGE FORMS

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Published: 2021-11-19

Page: 416-425


S. K. SABEEHA BEGUM

Department of Chemistry, Maris Stella College (A) Vijayawada 520008, Acharya Nagarjuna University, Nagarjuna Nagar 522510, Guntur, Andhra Pradesh, India.

N. BARATHA JYOTHI *

Department of Zoology, Maris Stella College (A) Vijayawada 520008, Acharya Nagarjuna University, Nagarjuna Nagar 522510, Guntur, Andhra Pradesh, India.

R. TULASI

Department of Mathematics, Maris Stella College (A) Vijayawada 520008, Acharya Nagarjuna University, Nagarjuna Nagar 522510, Guntur, Andhra Pradesh, India.

R. SARALA

Department of Electronics, Maris Stella College (A) Vijayawada 520008, Andhra Pradesh, India.

G. SRAVANI

Department of Zoology and Aquaculture, Acharya Nagarjuna University, Nagarjuna Nagar 522510, Guntur, Andhra Pradesh, India.

*Author to whom correspondence should be addressed.


Abstract

A simple, rapid, precise, sensitive and, reproducible reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the quantitative analysis of Cefpodoxime in a pharmaceutical dosage form. Chromatographic separation of Cefpodoxime was achieved on Waters Alliance -2695, by using Luna Pheny Hexyl (250mm x 4.6mm, 5µm) column and the mobile phase containing 0.1% TEA adj pH-2.5 with OPA & ACN in the ratio of 75:25% v/v. The flow rate was 1.0 ml/min, detection was carried out by absorption at 222nm using a photodiode array detector at ambient temperature. The number of theoretical plates and tailing factor for Cefpodoxime were NLT 2000 and should not be more than 2 respectively. The linearity of the method was excellent over the concentration range 7-105 µg/ml for Cefpodoxime respectively. The correlation coefficient was 0.999. % Relative standard deviation of peak areas of all measurements always less than 2.0. The proposed method was validated according to ICH guidelines.  The method was found to be a simple, economical, suitable, precise, accurate & robust method for quantitative analysis of Cefpodoxime and study of its stability.

Keywords: HPLC, Cefpodoxime, stability, pharmaceutical dosage


How to Cite

BEGUM, S. K. S., JYOTHI, N. B., TULASI, R., SARALA, R., & SRAVANI, G. (2021). A STUDY ON DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF CEFPODOXIME IN BULK AND A PHARMACEUTICAL DOSAGE FORMS. Asian Journal of Advances in Medical Science, 3(1), 416–425. Retrieved from https://mbimph.com/index.php/AJOAIMS/article/view/2612

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