Published: 2022-05-25

Page: 172-178


Department of Biotechnology & Bioinformatics, Bishop Heber College, Tiruchirappalli 620017, India.


Department of Biotechnology & Bioinformatics, Bishop Heber College, Tiruchirappalli 620017, India.


Department of Biotechnology & Bioinformatics, Bishop Heber College, Tiruchirappalli 620017, India.


Department of Biotechnology & Bioinformatics, Bishop Heber College, Tiruchirappalli 620017, India.

*Author to whom correspondence should be addressed.


Polyunsaturated fatty acids (PUFAs) are a vital component for human health. PUFA cannot be synthesized by human system and hence dependance on other sources has become inevitable.

Though porcine liver and fish oil were the dependable sources for ages past, yet cheaper microbial source was sought after and have gained importance as well. Such microbial oils normally used as biofuels can also be used for several therapeutic purposes. Hence, this study was designed to evaluate the quality and quantity of lipids produced by a probiotic yeast, Rhodotorula mucilaginosa. The lipid production potential of Rhodotorula mucilaginosa has indeed attracted a lot of attention. Isolation and characterization of the probiotic yeast with enhanced lipid production and determining the lipid components have become the aim of the work.

Keywords: Rhodotorula mucilaginosa, single cell oil, lipid, oleaginous

How to Cite

JASMINE, R., GANESH, R., MOHANAPRIYA, S., & DHARANI, R. (2022). PROBIOTIC Rhodotorula mucilaginosa ISOLATED FROM FERMENTED FOOD: INVESTIGATION OF PUFA PRODUCTION AND STRATEGY FOR HEALTH IMPROVEMENT. Asian Journal of Advances in Medical Science, 4(1), 172–178. Retrieved from


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Eckel RH, Jakicic JM, Ard JD, et al. 2013 AHA/ACC guideline on lifestyle management to reduce cardiovascular risk: a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines. J Am Coll Cardiol. 2014;63(25 Pt B):2960-2984.

PMID: 24239922

Hensrud DD, Heimburger DC. Nutrition's interface with health and disease. In: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine. 26th ed. Philadelphia, PA: Elsevier; 2020:chap 202.

Corsetto PA, Montorfano G, Zava S, Jovenitti IE, Cremona A, Berra B, Rizzo AM. Effects of n-3 PUFAs on breast cancer cells through their incorporation in plasma membrane. Lipids Health Dis. 2011;10:73.

DOI: 10.1186/1476-511X-10-73.

Papanikolaou S. Oleaginous Yeasts: Biochemical Events Related with Lipid Synthesis and Potential Biotechnological Applications. Ferment. Technol. 2012;1:1–3. DOI: 10.4172/2167-7972.1000e103.

Kuan, IC, Kao, WC, Chen, CL, Yu CY. Microbial Biodiesel Production by Direct Transesterification of Rhodotorula glutinis Biomass. Energies. 2018;11(5):1036.


Bento TFSR, Viana VFM, Carneiro LM, Silva JPA. Influence of Agitation and Aeration on Single Cell Oil Production by Rhodotorula glutinis from glycerol. Journal of Sustainable Bioenergy Systems. 2019;09(02): 29–43.


Ito T, Nakashimada Y, Senba K, Matsui T, Nishio N. Hydrogen and ethanol production from glycerol-containing wastes discharged after biodiesel manufacturing process. Journal of Bioscience and Bioengineering. 2005; 100(3):260–265. Available:

Kot AM, Błażejak S, Kurcz A, Bryś J, Gientka I, Bzducha-Wróbel A, Reczek L. Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis. Electronic Journal of Biotechnology. 2017.27: 25–31. Available:

Zheng YYuX, Zeng J, Chen S. Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw. Biotechnology for Biofuels. 2012.5(1):50.


Kurtzman CP, Fell JW. The yeast a taxonomic study, 4th edition, Elsevier, Amsterdam. 1998; 77-102.

Pan LX, Yang DF, Shao LLiW, Chen GG, Liang ZQ. Isolation of the oleaginous yeasts from the soil and studies of their lipid-producing capacities. Food Technology and Biotechnology. 2009;47(2):215-220.

Kraisintu P, Yongmanitchai W, Limtong S. Selection and optimization for lipid production of a newly isolated oleaginous yeast, Rhodosporidium toruloides DMKU3-TK16. Kasetsart Journal (Natural Science). 2010; 44(1):436-445.

Sakaki H, Nakanishi T, Satonak KY, Miki W, Fujita T, Komemushi S. Properties of a high-torularhodin-producing mutant of Rhodotorula glutinis cultivated under oxidative stress. Journal of Bioscience and Bioengineering. 2000;89(2):203–205. Available:

Dowhan W, Bogdanov M. Functional roles of lipids in membranes, in Encyclopedia of Biophysics, ed G.C.K. Roberts (Berlin; Heidelberg: Springer Berlin Heidelberg). 2013;868–875.

Asadi SZ, Khosravi-Darani K, Nikoopour H, Bakhoda H. Evaluation of the effect of process variables on the fatty acid profile of single cell oil produced by Mortierella using solid-state fermentation. Crit.Rev. Biotechnol. 2015;35: 94–102.


Fakas, S, Papanikolaou S, Batsos A, Galiotou-Panayotou M, Mallouchos A, Aggelis G. Evaluating renewable carbon sources as substrates for single cell oil production by Cunninghamella echinulata and Mortierella isabellina. Biomass Bioener. 2009;33:573–580. DOI:10.1016/j.biombioe.2008.09.006

Chatzifragkou A, Makri A, Belka A, Bellou S, Mavrou M, Mastoridou M, et al. Biotechnological conversions of biodiesel derived waste glycerol by yeast and fungal species. Energy. 2011;36:1097–1108.

DOI: 10.1016/

Tchakouteu SS, Kalantzi O, Gardeli C, Koutinas AA, Aggelis G, Papanikolaou S. Lipid production by yeasts growing on biodiesel-derived crude glycerol: strain selection and impact of substrate concentration on the fermentation efficiency. J. Appl. Microbiol. 2015;118:911–927.

DOI: 10.1111/jam.12736

Chatzifragkou A, Fakas S, Galiotou-Panayotou M, Komaitis M, Aggelis G, Papanikolaou S. Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi. Eur. J. Lipid Sci. Technol. 2010;112:1048–1057.


Lin H, Cheng W, Ding H, Chen X, Zhou Q and Zhao Y. Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-in solid-state fermentation. Bioresour. Technol. 2010;101: 7556–7562. DOI:10.1016/j.biortech.2010.04.027

Matsakas L, Bonturi N, Miranda E, Rova U, Christakopoulos P. High concentrations of dried sorghum stalks as a biomass feedstock for single cell oil production by Rhodosporidium toruloides. Biotechnol. Biofuels. 2015;8-16.

DOI: 10.1186/s13068-014-0190-y

Bellou S, Triantaphyllidou IE, Aggeli D, Elazzazy AM, Baeshen MN, Aggelis G. Microbial oils as food additives: recent approaches for improving microbial oil production and its polyunsaturated fatty acid content. Curr. Opin. Biotechnol. 2016;37:24–35.

DOI: 10.1016/j.copbio.20 15.09.005

Laoteng K, Certik M. Biotechnological production and application of high-value microbial oils, in Industrial Fermentation: Food Processes, Nutrient Sources and Production Strategies (Hauppauge, NY: Nova Science Publishers, Inc). 2010;187–215.

Yu XC, Zheng YB, Dorgan KM, Chen SL. Oil Production by Oleaginous Yeasts Using the Hydrolysate from Pretreatment of Wheat Straw with Dilute Sulfuric Acid. Bioresour. Technol. 2011;102:6134–6140.

Huang C, Zong, MHWuH, Liu QP. Microbial Oil Production from Rice Straw Hydrolysate by Trichosporon fermentans. Bioresour. Technol. 2009;100:4535– 4538.

Amaretti A, Raimondi S, Sala M, Roncaglia L, Lucia M, Leonardi A, Rossi M. Single cell oils of the cold-adapted oleaginous yeast Rhodotorula glacialis DBVPG 4785. Microb Cell Fact. 2010;9:73.

Bialy HE, Gomaa OM, Azab KS. Conversion of oil waste to valuable fatty acids using oleaginous yeast. World J Microbiol Biotechnol; 2011.

DOI: 10.1007/s11274-011-0755-x

Gill CO, Hall MJ, Ratledge C. Lipid accumulation in an oleaginous yeast (Candida 107) growing on glucose in single-stage continuous culture. Appl Environ Microbiol. 1997;33:231–239.

Huang C, Chen XF, Xiong L, Chen XD, Ma LL, Chen Y. Single cell oil production from low-cost substrates: the possibility and potential of its industrialization. Biotechnol. Adv. 2013;31:129–139.

DOI: 10.1016/j.biotechadv.2012.08.010

Tournas VH, Heeres J, Burgess L. Moulds and yeasts in fruit salads and fruit juices. Food Microbiology. 2006;23(7):684– 688.


Tsigie YA, Wang CY, Truong CT, Ju YH. Lipid production from Y. lipolytica Po1g grown in sugarcane bagasse hydrolysate. Biores Technol. 2011;648(102):9216–9222.

Yu X, Dong T, Zheng Y, Miao C, Chen S. Investigations on cell disruption of oleaginous microorganisms: hydrochloric acid digestion is an effective method for lipid extraction. Eur. J. Lipid Sci. Technol. 2015; 117:730–737.

DOI: 10.1002/ejlt.201400195,

Zhu L, Zong M, Wu H. Efficient lipid production with Trichosporon fermentans and its use for biodiesel preparation. Biores Technol. 2008;99:7881–7885.

DOI: 10.1016/j.biortech.2008.02.033