EFFECT OF OLIVE OIL EXTRACT ON LEAD INDUCED IN MICE, THE STUDY OF GLYCEMIA AND LIPIDEMIA IN NORMAL AND DIABETIC TREATED MICE
Asian Journal of Advances in Research, Volume 4, Issue 1,
Page 29-40
Abstract
Lead is one of the environmental contaminant, which can threaten living organism in many ways. Mice treated with lead acetate found to exhibit significant production in hemoglobin content between normal and experimentally treated mice. The hematological values indicated a significant production in total white blood cells (WBC), red blood cells (RBC) count between normal and experimental. The level of high-density lipoprotein (HDL) in lead induced mice was found to be low with18.0 mgs/dl where as the control mice revealed an elevated high-density lipoprotein (HDL) level of 32.0 mgs/dl. Similarly the level of low-density lipoprotein (LDL), cholesterol was found be elevated than the control. Another biochemical parameter is the estimation of serum cholesterol which was found to be 90.0 mgs/dl with lead acetate induced mice and a remarkable decrease in serum cholesterol was established which exhibited 65.0mgs/dl. The results for serum triglycerides with lead induced mice showed 49.5 mgs/dl than compared to normal mice. Analysis of serum biomarkers such as lactate dehydrogenase (LDH) revealed an increased lactate dehydrogenase (LDH) level than the normal control mice. To study the kidney dysfunction the level of serum creatinine revealed an elevated level of 0.90 mgs/dl which is the remarkable effect leading to renal failure. The level of total bilirubin was found be eye in lead induced mice than the control treated mice. Studies pertaining to body weight absorbed during the periods of 15 days it was found be reduced in lead induced toxicity mice. To study of diabetic condition induced with streptozotocin (STZ) over a period of 15 days which exhibited significance rise in blood glucose concentration than the normal control mice. The study further extended the supplementation of olive oil in order to toxicity of lead acetate by olive oil revealed serum biomarker such as level of serum glutamate-pyruvate transminase (SGPT) in control showed 46.0 U/ML. Whereas in olive oil treated mice along with STZ induced mice revealed a moderate enzyme activity. Antipyretic study of olive oil by assessing the rectal temperature showed 40°C for control where as olive oil administered mice showed a rectal temperature of 40.5°C.
- Lead
- hematological
- antipyretic
- Lactate Dehydrogenase (LDH)
- Low Density Lipoprotein (LDL)
- High Density Lipoprotein (HDL)
- Serum Glutamate-pyruvate Transminase (SGPT)
- Serum Glutamatic-oxaloacetic Transaminase (SGOT) and Streptozotocin.
How to Cite
References
Hamlet J, Demuth k, Paul JL, Derabar JM, Janel N. Hyperhomocysteinemia due to cystathionine Beta Synthase Deficiency Induces Dysregulation of Genes Involved in Hepatic Lipid Homeostasis in mice. Journal of Hepatology. 2007;46:151-1 59.
Olabisi T Obafemi, Onasanya Amos, Akinwunmi Adeoye, John Adeolu Falode. Protective effect of methanolic and flavonoid-rich leaf extracts of Synsepalum dulcificum (Danielli) on lead-acetate-induced toxicity in Wistar albino rats ARTICLE INFO. Journal of Applied Pharmaceutical Science. 2009;9(5):65-072.
DOI: 10.7324/JAPS.2019.90508.
Wani FA, Rahians SS, Tantry BA. Evaluatin of anti. Asthatic effect of extra virgin olive oil (Oleaeuropea) against milk induced leukocytosis and eosinophilia. Advances in Bio-reaserch. 2015;6:15-18.
Debamita Kilikdar U, Debasri Mukherjee, Elina Mitra, Arnab Kumar Ghosh, Anjali Basu, Ananga Mohan Chandra, Debasish Bandyopadhyay. Protective effect of aqueous garlic extract against lead-induced hepatic injury in rats. Indian Journal of Experimental Biology. 2011;49(7):498-510.
Smith DR, osterloh JD, Flegal AR. Use of endogenous, stable lead isotopes to determine release of lead from the skeleton. Environ Health Perspect. 1996;104:60-66.
Russell Jones R. The continuning hazard of lead in drinking water. Lancet. 1989;2:669-670.
Levin SM, Gold berg M. Clinical evaluation and management of lead –exposed construction workers. Am J Ind Med. 2003;37:23-43.
Ariza ME, Bijur GN, Williams MV. Lead and mercury mutagenesis, role of H2O2. Superoxide dismutase, and xanthine oxidase. Environ Mol Mutagen. 1998;31:352-61.
Pirkle JL, Schwartz J, Landis R, Harlan WR. The relationship between blood lead levels and blood pressure and its cardiovascular risk implications.Am J Epidemiot. 1985;126:246-258.
Ali MA, Awal MA, Mostofa M, Islam MA, Ghosh A. Effects of selineum and vitamin B6 with their combination lead acetate induced texocities in long even rats. Bangl J Vet Med. 2010;8:63-73.
Rous P. The effect of heavy metals boundary contaminated soil on haematological and selected biochemical parameters in blood plasma of rabbits. Acta-Universitais- Agriculturae- et-Silviculturae-Mendelianae-Brunesis. 2000;48:93-99.
Yagminas AP, Franklin CA, Villeneuve DC, Gilman AP, Little PB, Valli VE. Subchronic oral toxicity of triethyl lead in the male weanling rat. Clinical, biochemical, hematological, and histopathalogical effects. Fundam Appl Toxical. 1990;15:580-596.
Sudakova AI, Shevchenko ZT, Nosova LI, Peripheral blood and bone marrow cell status of white rats with long-term lead exposure. Tsitol Genet. 1983;17:3-7.
Benavente- Garcia O, Gastillo J, Lorente J, Ortuno A, Del Riao JA. Antioxidant activity of phenolics extracted from ouropaea L. leaves. Food Chem. 2000;68:457-462.
Bianco A, Uccella N. Biophenolic components of olives. Food Res. 2002;3:475-487.
Khayyal MT, EL- Ghazaly MA, Abdallah DM, Nassar NN, Okponyi SN, Kreuter MH, Blood pressure lowering effect of an olive leaf extract (olea europaea) in L-NAME induced hypertension in rats. Arzneimittelforsching. 2002;52:797-802.
Somoa LI, Shode FO, Ramanan P, Nadar A. Antihypertensive, antiatherosclerotic and antioxidant activity of triterpenoids isolated from Olea eurpaea, subspecies Africana leaves. J. Ethnopharmacol. 2003;84:191-198.
Skerget M, Kotnik P, Hadolin M, Hradolin AR, Simoni M, Kenz Z. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem. 2005;89:191-198.
Sudjana AN, D Orazio C, Ryan V, Rasool N, Ng J. Islam N, Riley TV, Hammer KA. Antimicribial activity of commercial Olea europaea(olive) leaf extract. Int J Antimicrobial Agents. 2009;33:461-463.
Floch FL, Tena MT, Rios A, Valcarcel M. Supercritical fluid extraction of phenol compounds from olive leaves. Talanta. 1998;46:1123-1130.
Briante R, Febbraio F, Nucci R. Antioxidant properties of low molecular weight phenols present in the meditterranean diet. J. Agric Food Chem. 2003;51:6975-6981.
Marilia V, Piasek M, Blanusa M, Saric M, Juresa D, Kostial K. Succimer treatment and calcium supplementation reduce tissue lead in suckling rats. JAppl Toxicol. 2004;24:123-128.
Rabinowitz MB, Wetherill GW, Kopple JD. Kinetic analysis of lead metabolism in healthy humans. J.Clin Invest. 1976;58:260-270.
Correia PRM, Oliverira E, Oliverira PV. Simmultaneou determination of cd and Pb in food stuffs by electro-thermal atomic absorption spectrometry Anal Chim Acta. 2000;405:205-211.
Shalan MG, Mostafa MS, Hasouna MM, Nabi SE, Refaie A. Amelioration of lead toxicity on rat liver with vitamin c and silymarin supplements. Toxicology. 2005;206:1-15.
Shalan MG, Mostafa MS, Hasouna MM, Nabi SE, Refaie A. Amelioration of lead toxicity on rat liver with vitamin c and silymarin supplements. Toxicology. 2005;206:1-15.
Elayat W, Bakheetf MS. Effects of chronic lead toxicity on liver and kidney functions. J Med Lab Sci. 2010;1:29-36.
Hernberg S. Biochemical and clinical effects and response as indicated by blood concentrations. In signal, R.L., Thomas, T.A., (Eds). Lead Toxicity. Baltimre, M.D., urban and schwarzenberg. 1980;367-399.
Thulesen J, Qrskovic C, Holst JJ, Poulsen SS. Short term insulin prevents the diabetogenic action of streptozotocin in rats. Endocrinology. 1997;138(1):62-8.
Zhang XZ, Su YS, Yu DJ. Effects of acute exposure to lead on behaviors and lipid peroxidation in mice. Guang dong Welliang Yuansu Kexue. 2007;14(12):30-35.
-
Abstract View: 859 times
PDF Download: 463 times
