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Published: 2021-03-24

Page: 216-224


Department of Cell Biology, National Research Centre, 33 El-Bohouth St. (Former El Tahrir St.), Dokki, P.O. 12622, Giza, Egypt.


Department of Cell Biology, National Research Centre, 33 El-Bohouth St. (Former El Tahrir St.), Dokki, P.O. 12622, Giza, Egypt.

*Author to whom correspondence should be addressed.


Liver regeneration after partial hepatectomy (PH) is one of the most captivating phenomena in medicine. The aim of this work was to study the effect of time (days) on DNA damage, micronucleus, liver contents (weight, water and lipid %) and function after PH in male rats was evaluated.  Liver weight, water and lipid contents as well transaminases enzymes, bilirubin (BR) and protein were determined. In addition, DNA fragmentation, micronucleus (Mn) induction and hemoglobin were also assessed. Liver weight was decreased significantly and restored to normal by day 3. However, lipid content was increased significantly but restored to normal by day 7. Water content was similarly increased in regenerating liver and restored the normal by day 28. Transaminases, Brill and protein levels were decreased and restored to normal by day 14. In addition, hemoglobin was decreased and restored to normal by day 28. DNA fragmentation and micronucleus frequency were increased significantly compared to control (day 0) and restored to normal on day 7 and 28 for DNA and Mn, respectively. In conclusion, liver regeneration in male rats after 70% partial hepatectomy (PH), response at 3rd, 7th, and 14th days. Our results might be of clinical relevance for human medicine.

Keywords: Liver regeneration, Partial Hepatectomy (PH), DNA fragmentation, function, liver contents, rats

How to Cite

ELRAOUF, A. A., & GIRGIS, S. M. (2021). POTENTIAL IMPACT OF TIME ON DNA DAMAGE, MICRONUCLEUS WITH LIVER CONTENTS AND FUNCTION AFTER PARTIAL HEPATECTOMY IN RATS. Asian Journal of Advances in Research, 4(1), 216–224. Retrieved from https://mbimph.com/index.php/AJOAIR/article/view/1996


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Gravitz L. Liver cancer. Nature. 2014;516:S1.

Bruix J, Han KH, Gores G. et al. Liver cancer: Approaching a personalized care. Journal of Hepatology. 2015;62:144-156.

DeSantis CE, Siegel RL, Sauer AG. et al. Cancer statistics for African Americans, 2016: Progress and opportunities in reducing racial disparities. Cancer Journal of Clinicians. 2016;66:290-308.

Siegel RL, Miller KD, Jemal A. Cancer statistics. Cancer Journal of Clinicians. 2016;66:7-30.

Liu CY, Chen KF, Chen PJ. Treatment of liver cancer. Cold Spring Harbor Perspectives in Medicine. 2015;5:a021535.

Rmilah AA, Zhou W, Nelson E, Lin L, Amiot B, Nyberg SL. Understanding the marvels behind liver regeneration. Wiley Interdisciplinary Reviews: Developmental Biology. 2019;8(3):1-46.

Michalopoulos GK, DeFrances MC. Liver regeneration. Science. 1997;276: 60-66.

Taub R. Liver regeneration: from myth to mechanism. Nature Reviews Molecular Cell Biology. 2004;5:836-847.

Michalopoulos GK, Khan Z. Liver regeneration, growth factors, and amphiregulin. Gastroenterology. 2005;128: 503-506.

Michalopoulos GK. Liver regeneration. Journal of Cellular Physiology. 2007;213:286-300.

Fausto N, Campbell JS, Riehle KJ. Liver regeneration. Hepatology. 2006;43:S45–S53.

Higgins GM, Anderson RM. Experimental pathology of the liver I restoration of the liver of the white rat following partial surgical removal. The Archives of Pathology. 1931;12:186-202.

Popper H. Liver disease-morphologic considerations. The American Journal of Medicine. 1954;16:98-117.

Weinbren K. The pathology of hepatitis. The Journal of Pathology and Bacteriology. 1952;64:395-413.

Andersen KJ, Knudsen AR, Kannerup AS, Sasanuma H, Jens Nyengaard JR, Dutoit SH, Erlandsen EJ, Jørgensen B, Mortensen FV. The natural history of liver regeneration in rats: Description of an animal model for liver regeneration studies. International Journal of Surgery. 2013;11:903-908.

Sostman HD, Gore JC, Flye MW, Johnson GA, Herfkens RJ. Time course and mechanism of alterations in Proton relaxation during liver regeneration in the rat. Hepatology. 1985;5(4):538-543.

Carrillo MC, Carnovale CE, Favre C, Monti JA, Scapini C. Hepatic protein synthesis and serum amino acid levels during liver regeneration in young and old malnourished rats. Mech. Ageing and Development. 1996;91:55-64.

Cajone F, Bernelli-Zazzera A. Protein synthesis in regenerating liver. International Journal of Biochemistry. 1980;12:537-544.

Huang W, Wang Y, Jiang X. et al: Protective effect of flavonoids from Ziziphus jujuba cv. Jinsixiaozao against acetaminophen-induced liver injury by inhib¬iting oxidative stress and inflammation in mice. Molecules. 2017;22(10):E1781.

Shin YH, Ko JS, Kim GS. et al. Impact of hepatic macrovesicular and microve¬sicular steatosis on the postoperative liver functions after right hepatecto¬my in living donors. Transplantation Proceedings. 2012;44:512-15.

Guide for the Care and Use of Laboratory Animals. National Research Council (US). Institute for Laboratory Animal Research: National Academies Press (US). Washington (D C); 1996.

Mitchell C, Willenbring H. A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice. Nature Protocols. 2008;3:1167-1170.

Tates AD, Neuteboom I, Hofker M, den Engels L. A Micronucleus technique for detecting clastogenic effects of mutagens/carcinogens (DEN, DMN) in hepatocytes of rat liver in vivo. Mutation Research. 1980;74:11-20.

Ozeki A, Tsukamoto I. Retinoic acid repressed the expression of c-fos and c-jun and induced apoptosis in regenerating liver after partial hepatectomy. Biochimica et Biophysica Acta. 1999;1450:308-319.

Suzuki T, Tsukamoto I. Apoptosis induced by 5-(N,N-hexamethylene)-amiloridein regenerating liver after partial hepatectomy. European Journal of Pharmacology. 2004; 503:1–7.

Jaffe JJ. Diurnal mitotic periodicity in regenerating rat liver. The Anatomical Record. 1954;120:935-954.

Sherr CJ. G1 phase progression: cycling on cue. Cell. 1994;79:551-555.

Sherr CJ, Roberts JM. Inhibitors of mammalian G1 cyclin-dependent kinases. Genes and Development. 1995;9:1149-1163.

Rohlfing AK, Trescher K, Hähnel J, Müller C, Hildebrandt JP. Partial hepatectomy in rats results in immediate down-regulation of p27Kip1 in residual liver tissue by transcriptional and post-translational processes. Frontiers in Physiology. 2013;4(139): 1-10.