TOXICITY ACTIVITIES OF Jatropha curcas SEED EXTRACT AGAINST THE 4TH INSTAR LARVAE AND PUPAL STAGES OF Aedes aegypti

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Published: 2021-10-04

Page: 1018-1022


VICTOR S. NJOM *

Department of Applied Biology and Biotechnology, Enugu State University of Science and Technology, P.M.B. 1660, Enugu, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Mosquitoes are vectors of several diseases of public health importance. Their control with synthetic insecticides has faced challenges of resistance by target insects and consequent environmental pollution. There is therefore need to find alternative ecofriendly plant derived insecticide. Therefore toxicity activities of Jatropha curcas seed extract against the 4th instar larvae and pupal stages of Aedes aegypti was studied in vitro with increasing doses of Jatropha curcas seed extract for 96 hours. The result showed significant difference between mortality among treated animal and control (P< 0.05). Mortality of both larvae and pupae of Aedes aegypti treated with Jatropha curcas seed extract was dose and time dependent. At the highest (9%) concentration (wt/vol), mean larval mortality was 100%, while at the lowest (1%) concentration, the mortality decreased to 50%. Mean pupal mortality increased to 100% at highest concentration of 9% (g/100ml) but decreased to 40% at lowest concentration. The duration of incubation also influenced mortality. At 72 and 96 hours of incubation all treated larva died in highest concentrations of the extract (9% and 7%) while the entire treated pupa died out in 96 hours of incubation at the highest concentration (9%) of the extract. Survived larva and pupa after 96 hours in lower concentration could not develop to adult stage. In conclusion Jatropha curcas seed water extract has toxicity effects on larvae and pupae of Aedes aegypti and can be deployed for the control of the insect.

Keywords: Toxicity, Jatropha, vector, insecticides, Aedes aegypti


How to Cite

NJOM, V. S. (2021). TOXICITY ACTIVITIES OF Jatropha curcas SEED EXTRACT AGAINST THE 4TH INSTAR LARVAE AND PUPAL STAGES OF Aedes aegypti. Asian Journal of Advances in Research, 4(1), 1018–1022. Retrieved from https://mbimph.com/index.php/AJOAIR/article/view/2505

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References

Bassem F, Larbi KM, Hela E. Larvicidal efficacy of Jatropha curca L. (Euphorbiaceae) leaf and seed aqueous extracts against Culex pipiens L. African Journal of Biotechnology. 2014;13.

Gutierrez PM, Antepuesto AN, Eugenio BAL, Santos MFL. Larvicidal activity of selected plant extracts against the dengue vector Aedes aegypti mosquito. Int Res J Biol Sci. 2014;3: 23-32.

Lee H, Halverson S, Ezinwa N. Mosquito-borne diseases. Primary Care: Clinics in Office Practice. 2018;45:393-407.

Colón-González FJ, Sewe MO, Tompkins AM, Sjödin H, Casallas A, Rocklöv J, Caminade C, Lowe R. Projecting the risk of mosquito-borne diseases in a warmer and more populated world: a multi-model, multi-scenario intercomparison modelling study. The Lancet Planetary Health. 2021;5:e404-e414.

Nosrat C, Altamirano J, Anyamba A, Caldwell JM, Damoah R, Mutuku F, Ndenga B, Labeaud AD. Impact of recent climate extremes on mosquito-borne disease transmission in Kenya. PLOS Neglected Tropical Diseases. 2021;15: e0009182.

Martinez J, Showering A, Oke C, Jones RT, Logan JG. Differential attraction in mosquito– human interactions and implications for disease control. Philosophical Transactions of the Royal Society B: Biological Sciences. 2021;376:20190811.

Njom VS, Umeh EDN, Eze CS. Assessment of the insecticidal potency of neem (Azadirachta indica) seed kernel methanolic and aqueous extracts on the malaria vector Anopheles gambiae. Animal Research International. 2011; 8:1392-1397.

Mishra P, Tripathi A, Dikshit A, Pandey A. Insecticides derived from natural products: diversity and potential applications. Natural Bioactive Products in Sustainable Agriculture. Springer Singapore; 2020.

Ara ZG, Haque AR. A Comprehensive review on synthetic insecticides: toxicity to pollinators, associated risk to food security, and management approaches. Journal of Biosystems Engineering. 2021;1-19.

Kumar R, Sankhla MS, Kumar R, Sonone SS. Impact of pesticide toxicity in aquatic environment. Biointerface Research in Applied Chemistry. 2021;11:10131-10140.

Uche AE, Ndubuaku TCN. Natural Plant Products as Potential Synthetic Insecticide Alternatives in Developing African Countries. Biopesticides in Organic Farming. CRC Press; 2021.

Godlewska K, Ronga D, Michalak I. Plant extracts-importance in sustainable agriculture. Italian Journal of Agronomy. 2021;16.

Bourogâa F. Insecticidal properties of the physic nut tree Jatropha curcas L. (Euphorbiaceae) and potential use in pest management. Georg-August-Universität Göttingen; 2018.

Opuba SK, Adetimehin AD, Iloba BN, Uyi OO. Insecticidal and anti-ovipositional activities of the leaf powder of Jatropha curcas (L.) (euphorbiaceae) against Callosobruchus maculates (F.) (Coleoptera: Chrysomelidae). Animal Research International. 2018;15:2971–2978.

Evanjelene VK, Velu G. Preliminary phytochemical, proximate and nutrient analysis indifferent leaf extracts of Jatropha curcas L. Indian Journal of Pharmacy & Drugs Studies. 2021;7-14.

Babil YB. Environmental, Economical and Social Implication of Jatropha: A Systematic Review; 2021.

Abobatta WF. Jatropha curcas, a Novel Crop for Developing the Marginal Lands. Biofuels and Biodiesel. Springer; 2021.

Silva GN, Faroni LRA, Sousa AH, Freitas RS. Bioactivity of Jatropha curcas L. to insect pests of stored products. Journal of Stored Products Research. 2012;48:111-113.

Makkar H, Becker K, Sporer F, Wink M. Studies on nutritive potential and toxic constituents of different provenances of Jatropha curcas. Journal of Agricultural and Food Chemistry. 1997;45:3152-3157.

Okweche SI, Hilili PM, Ekoja EE. Termiticidal activity of oil from Jatropha curcas L. and Azadirachta indica A. Juss against Coptotermes sjostedti Holmgren (Isoptera: Rhinotermitidae). Bulletin of the National Research Centre. 2021;45:1-8.

Jide-Ojo C, Gungula DT, Ojo OO. Extracts of Jatropha curcas L. exhibit significant insecticidal and grain protectant effects against maize weevil, Sitophilus zeamais (Coleoptera: Curculionidae); 2013.

Chaieb I. Saponins as insecticides: a review. Tunisian Journal of Plant Protection. 2010;5: 39-50.

Roopashree K, Naik D. Saponins: Properties, applications and as insecticides: A review. Biosci. Trends. 2019;8:1-14.

Haq A, Mushtaq S, Khan A, Islam A, Khan H, Malik ZA, Younas F, Khan S, Shah AA, Badshah M. Evaluation of phytochemical, bioactive, and antifungal potential of Jatropha curcas seed oil and de-oiled seed cake extracts against phytopathogenic fungi. Journal of Plant Pathology. 2021;1-11.

Karamanoli K, Bouligaraki P, Constantinidou HI, Lindow S. Polyphenolic compounds on leaves limit iron availability and affect growth of epiphytic bacteria. Annals of Applied Biology. 2011;159:99-108.