Full Article - PDF

Published: 2022-02-12

Page: 249-261


Plant Protection Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.


Plant Protection Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.


Plant Protection Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.

*Author to whom correspondence should be addressed.


Under laboratory conditions; the spiny bollworm, Earias insulana (Boisd.) treated with LC50,s of the fungal compound, Beauvaria bassiana (Balsamo) exposed to gamma doses (400 & 700 Gy) and prepared in magnetized water (180 mlt). Assays of toxicity, biological and biochemical of E. insulana treated as newly hatched larvae with B. bassiana in different six treatments were done. B. bassiana + 400 or 700 Gy + magnetized water were the most effective treatments on the most E. insulana larvae assays used. While, B. bassiana + 400 or 700 Gy without preparing in magnetized water had the lower effect comparing with the two aforementioned treatments. Meanwhile, B. bassiana treatment when used singly caused the least effective on E. insulana larvae different assays.

At 5- day post treatment, the compound of B. bassiana + 700 Gy + magnetized water was the best treatment caused the highly toxicity (LC50: 8x102 IU) on E. insulana larvae. Also, the same treatment had drastically biological effect on larval and pupal stages. Addition, total carbohydrate, followed by total lipid and proteins of E. insulana had drastically decreased with the mentioned treatment; while, free amino acids increased than untreated. Meanwhile, all the assayed enzymes had the highly decreased compared with untreated, especially aspartate amino transferase (AST/GOT), followed by alanine aminotransferase (ALT/GPT), acetyle cholinesterase, trehalase, invertase and amylase.

So, B. bassiana exposed to gamma doses (400 & 700 Gy) and prepared in magnetized water can potentiate the fungal compound used on E. insulana larvae to be effective than B. bassiana when used singly.

Keywords: Earias insulana, Beauvaria bassiana, gamma ray, magnetized water, toxicity, biological, biochemistry

How to Cite

KANDIL, M. A. A., AMER, R. A. M., & AHMED, A. F. (2022). MAGNETIC FLUX AND GAMMA RAY CONJUNCTION IMPACTS WITH Beauvaria bassiana (BALSAMO) ON CERTAIN ASSESSMENTS OF Earias insulana (BOISD.). Asian Journal of Advances in Research, 5(1), 249–261. Retrieved from


Download data is not yet available.


Khan RR, Ahmed S, Saleem MW, Nadeem M. Field evaluation of different insecticides against spotted bollworms Earias spp. at district sahiwal. Pakistan Entomology. 2007;29(2):129-134.

Dannon HF, Dannon E, Douro-Kpindou OK, Zinsou AV, Houndete AT, Toffa-Mehinto J, Elegbede IA, Olou BD, Tamo M. Toward the efficient use of Beauveria bassiana in integrated cotton insect pest management. Journal of Cotton Research. 2020;3:24-45.

Long DW, Drummond GA, Groden E. Horizontal transmission of Beauvaria bassiana. Agriculture and Forest Entomology. 2000;2:11-17.

Prasad A, Syed N. Evaluating prospects of fungal biopesticide, Beauvaria bassiana (Balsamo) against Helicoverpa armigera (Hubner): An Eco safe strategy for pesticidal pollution. Asian Journal of Expermintal Biological Science. 2010;1(3):596-601.

Soon LG. Diamondback moth management (Proc. 1st Int. Workshop), Asian Research and Development Center, Taipei. 1986;159.

Amer RA, Abdel-Samad SS, Ahmed MA. Toxicity of Beauveria bassiana (Balsamo) exposed to gamma irradiation doses on some pests. Journal of Egyptian German Society Zoology. The 19th international conference 30 April-2 May 2011, Faculty of Science, Beni-Sueif University. 2011;63:33-47.

Amer RA, Yacoub ShS, Nouh GM, Hatem AE. Gamma irradiation to potentiate some bio-agents compounds against the cotton leaf worm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). Egyptian Journal Biological Pest Control. 2015;25(2):445-455.

Amer RA, Kandil MA, El- Shenawy RM. Comparison between gamma rays and magnetic flux effects on biological and life table assays of Earias insulan (Boisd.) eggs. Egyptian Academic Journal of Biological Science. 2019a;12(3):121-131.

Amer RA, Hassan NN, Sheba OG, Abdel-Salam DA. Gamma radiation potency to potentiate some compounds for controlling three cotton boll pests. Egyptian Academic Journal for Biological Science. 2019b; 11(3):1-13.

Barrya JF, Turner MJ, Schloss JM, Glenna DR, Songe Y, Lukinb MD, Park H, Walswortha RL. Optical magnetic detection of single-neuron action potentials using quantum defects in diamond. PNAS. 2017;113(49):14133-14138. PNAS

Amer AE. Economic artificial diets for rearing spiny bollworm, Earias insulana (Boisd.) (Lepidoptera: Noctuidae). Journal of Plant Protection and Pathology, Mansoura University. 2015;6(3):527-534.

Abbott WW. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology. 1925;18:265-267.

Finney DJ. Probit analysis. 3rd Edn. Cambridge University press, Cambridge, UK. 1971; 318.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 1976;72: 248-254.

Knight JA, Anderson S, Rawle R. Chemical basis of the sulfo-phospho-vanillin reaction for estimating total serum lipids. Clinic Chemistry. 1972;18:199-202.

Crompton M, Birt LM. Changes in the amounts of carbohydrates, phosphogen and related compounds during the metamorphosis of blowfly, Lucilla cupring. Journal of Insect Physiology. 1967;13:1575-1595.

Ishaaya I, Swirski E. Trehalase, invertase and amylase activities in the black scale saissetiaoleae and their relation to host adaptability. Journal of Insect Physiology. 1976;22:1025-1029.

Waterhouse DF, Hackman RH, McKellar JW. An investigation of chitinase activity in cockroach and termite extracts. Journal of Insect Physiology. 1961;6:96-112.

Simpson DR, Bull DL, Linquist DA. A semimicro technique for the estimation of cholinesterase activity in bull weevil. Annals of the Entomological Society of America. 1964;57:367-377.

Costat Statistical Software. Microcomputer program analysis version 4.20, cohort Software, Berkeley, CA; 1990.

Duncan DB. Multiple ranges and multiple F.test. Biomerics. 1955;11:1-42.

Aparecida K, Aquino S. Sterilization by gamma Irradiation, gamma radiation, Prof. Feriz Adrovic (Ed.), In Tech, 2012. ISBN: 978-953-51-0316-5. Available: gamma radiation/ sterilization by gamma irradiation

Quiun CJ, Molden TC, Sanderson CH. Magnetic treatment of water prevents mineral Buildup; 1997. Available:www.Superiorawaterconditioner.Com

Ghorbani S, Gholizadeh M, De-Brito J. Effect of magnetized water on the mechanical and durability properties of concrete block pavers. Materials (Basel). 2018;(11):1–16.

Ghorbani S, Tao Z, De-Brito J, Tavakkolizadeh M. Effect of magnetized water on foam stability and compressive strength of foam concrete. Construction and Building Materials. 2019;197:280–290.

Thenua K, Shanmugasundaram M, Karthiyain S. Investigation on performance of water ion modified cement matrix. National Science, Engineering and Technology Conference (NCSET) 2020; Journal of Physics: Conference Series IOP Publishing. 2021;1716-1721.

Wyatt GR. The biochemistry of sugars and polysaccharides in insects. Advanced of Insect Physiology. 1967;4:287-360.

El-Ghar GE, Radwan HS, El-Bermawy ZA, Zidan LT. Inhibitory effect of thuringiensin and abamectin on digestive enzymes and non-specific esterases of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae. Journal of Applied Entomology. 1995;119:355-359.

Eid AM. Esterases and phosphatases in relation to chlorpyrifos- resistance in Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). Egyptian Journal of Applied Science. 2002;17:275-284.

Mead HM, El-Sheakh AA, Soliman BA, Desouky WM, Abo-Ghalia AH. Biochemical effect of some compounds on carbohydrate hydrolyzing enzymes of cotton leafworm, Spodoptera littoralis (Boisd.). Egyptian Journal of Agriculture Research. 2008;86:2169- 2192.

Kandil MA, El-Shenawy RM, Amer RA. Impact of magnetic fields and temperatures on biological, life table, morphological and biochemical parameters in Earias insulana (Boisd.). Egyptian Journal of Agriculture Research. 2018;96(3):967-985.

Husseini MM. Effect of the fungus, Beauveria bassiana (Balsamo) Vuillemin, on the beet armyworm, Spodoptera exigua (Hübner) larvae (Lepidoptera: Noctuidae), under laboratory and open field conditions. Egyptian Journal of Biological Pest Control. 2019;29:52-56.

Bahmani N, Latifian M, Ostovan H, Hesami S.cPathogenic effects of Beauveria bassiana and Bacillus thuringiensis on the population dynamics of Ephestia Kuehniella. Egyptian Journal of Biological Pest Control. 2020;30:94-102.

Hamama HM, Zyaan OH, Abu Ali OA, Saleh DI, Elakkad HA, El-Saadony MT, Farag ShM. Virulence of entomopathogenic fungi against Culex pipiens: Impact on biomolecules availability and life table parameters. Saudi Journal of Biological Sciences. 2022;29:385- 393.