FUNGI ASSOCIATED WITH SPOILAGE OF PINEAPPLE (Ananas comosus L) FRUIT SOLD IN ANAMBRA STATE, NIGERIA

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Published: 2022-04-27

Page: 796-801


EJIMOFOR CHIAMAKA FRANCES

Department of Biological Sciences, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.

OLEDIBE ODIRA JOHNSON *

Department of Botany, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

The edible fruit of the tropical plant known as Pineapple (Ananas comosus) is commonly grown in Nigeria. Its high sugar content and low pH values make it susceptible to fungi. This study aims to identify those that can cause the spoilage of the fruit in the state. Five markets in Anambra state were visited to collect samples of pineapple (Eke Awka, Nnamdi Azikiwe temporary site (Temp. site), Nnewi, Uli, and Ihiala). They were then transported to a research facility in Awka, where they were studied for the fungi that cause the fruit's spoilage. The samples were treated with the standard procedure. The culture media used for the study were potato dextrose agar (PDA) and Sabouraud dextrose agar (SDA). Both were placed into a conical flask and subjected to an autoclave at a rate of 121ºC at 15psi for 20 minutes. In the study, various fungi that can cause the fruit's spoilage were identified, which included the Fusarium sp, Mucor sp, Aspergillus sp and Penicillium sp. Samples from Ihiala had the highest fungal counts (57 x 102 0.017a cfu/g) on PDA while samples from Temp. site had the highest fungal count (99 x 102 1.112a cfu/g) on SDA. Also, samples from Uli had the lowest fungal counts (22.00 x1020.111d cfu/g) on PDA while samples from Nnewi had the highest fungal count (36.00 x1020.100e cfu/g) on SDA. Aspergillus sp (47.91%) had the highest frequency of occurrence from the fungi isolated while Fusarium sp (10.42%) had the least occurrence. From the study, Aspergillus sp is the fungi specie that is responsible for the spoilage of pineapple fruits in the locations studied in Anambra State although other species of fungi can also affect pineapple fruits.

Keywords: Pineapple, Fungi, nutrition, media


How to Cite

FRANCES, E. C., & JOHNSON, O. O. (2022). FUNGI ASSOCIATED WITH SPOILAGE OF PINEAPPLE (Ananas comosus L) FRUIT SOLD IN ANAMBRA STATE, NIGERIA. Asian Journal of Advances in Research, 5(1), 796–801. Retrieved from https://mbimph.com/index.php/AJOAIR/article/view/2981

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References

Osei-Kofi F, Amoatey HM, Lokko Y. Improvement of pineapple using biotechnology and mutation breeding techniques. In: Report of 2 FAO/IAEA Research coordination meeting on ‘invitro techniques for selection of randiation-induced mutant adapted to adverse environmental conditions.’ 1997;23-27.

Kochhar S. Economic botany in the tropics, Macmillian Press, India. 1994;203.

Droby S, Chaluz E, Wilson CL, Nisneiweski M. Characterization of the biocontrol of Debaryomyces hensenii in the control of Penicillium digitatum on gapefruit. Can. J. Microbiol. 2006;35:794-800.

Nwachukwu E, Ezeama CF, Ezeanya BN. Microbiology of polythene packaged sliced pineapples sold by street vendors in Nigeria. Afr. Journal Microbiology Res. 2008;2:192-195.

Al- Hindi RR, Al-Najada AR, Mohammed SA. Isolation and identification of some fruits spoilage fungi African journal of microbiology Research. 2011;5(4):443-449.

Agrois G. Plant pathology (4th edition). Academic press, New York. 1997;63.

Debnath P, Dey P, Chanda A, Bhakta T. A survey of pineapple and its medicinal value. Scholar Acad. Journal Pharmacology. 2012; 1(1):143-149.

Onuorah SC, Udemezue OI, Uche JC, Okpoli IC. Fungi associated with spoilage of pineapple fruit. The Bioscientist. 2013;1(1):22- 27.

Hemalatha R, Anbuselvi S. Physicochemical constituents of pineapple pulp and waste. J. Chem. Pharm. Res. 2013;5(2):240-242.

Jolaosho AA, Kareem SO, Ogunmuyiwa SLO, Ajayi JO, Oseifeso OO. microbial analysis of sliced pineapple and paw paw in Ogun State, Nigeria. Journal of Medical and Applied Biosciences. 2010;2:112.

Akinmusire OO. Fungi species associated with spoilage of some edible fruits in Maiduguri. Advances in Environmental Biology. 2011; 5(1):157-161.

Chuku EC, Ogbonna DN, Onuegbu BA, Adeleke MTV. Comparative Studies on the Fungi and Bio-Chemical Characteristics of Snake Gaurd (Trichosanthes curcumerina linn) and Tomato (Lycopersicum esculentus mill) in Rivers state, Nigeria. Journal of Applied Sciences. 2008;8(1):168-172.

Effiuvwevwere BJO. Microbial Spoilage Agents of Tropical and Assorted fruits and Vegetables (An Illustrated References Book). Paragraphics publishing company, Port Harcourt. 2000;1-39.

Uzuegbu JO, Emifoneye AT. Post harvest Fungal Spoilage some Nigeria Fruits and Vegetables. Nigeria Food Journal. 1984;2 (1):153–155.

Varge J, Frisvad J, Samson R. A Reappraisal of fungi producing aflatoxins. World Mycotoxin J. 2009;2(3):263-277.

Amusa N, Shaye O, Oladapo M, Kafaru O. Preharvest deteriorations of soursop at Ibadan South Western Nigeria and its effect on nutrient composition. Afr. J. Biotechnol. 2003;2:23-25.