HISTOPATHOLOGICAL STUDIES ON THE EFFECT OF SOME ALTERNATIVE INSECTICIDES ON Spodoptera littoralis (Boisd.)

PDF

Published: 2022-07-22

Page: 946-954


EL SHAIMAA NAGUIB IBRAHIM ABD EL MAGEED *

Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt.

*Author to whom correspondence should be addressed.


Abstract

The insecticidal activity biological and histological effects of a bacterial bioagent spinosad (24% SC), an insect growth regulator methoxyfenozide (24% SC) and extrem (36 % SC), Ready-made mixture (Spinetoram 6% and methoxyfenozide 30%) on the 4th larval instar of the cotton leaf warm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae), under laboratory conditions, as denoted by the determined LC50 which was 7.28, 0.071 and 0.113 ppm, in spinosad, methoxyfenozide and extrem, respectively.  The highest rate of decrease in the pupation percentage of 25% was recorded in case of treated with extreme followed by spinosad and methoxyfenozide (76 and 63 % respectively). Also, the adult emergence rate was remarkably reduced in case of extreme to 40% followed by spinosad 56.6% and methoxyfenozide 69.8%. The male moths recorded lower rate of adult longevity than the female moths. On the other hand both toxicants induced drastic effect on fecundity and fertility of adult moths. Highly histopathological disturbances in the midgut of this pest including destruction of the muscle layers, disorganization in the epithelial cells, separation of the peritrophic membrane as well as detachment of the basement membrane and appearance of vacuolizations. Also caused severe histological aberration of the ovarioles.

Keywords: Spinosad, methoxyfenozide, extreme, Spodoptera littoralis, biological study, histology


How to Cite

MAGEED, E. S. N. I. A. E. (2022). HISTOPATHOLOGICAL STUDIES ON THE EFFECT OF SOME ALTERNATIVE INSECTICIDES ON Spodoptera littoralis (Boisd.). Asian Journal of Advances in Research, 5(1), 946–954. Retrieved from https://mbimph.com/index.php/AJOAIR/article/view/3094

Downloads

Download data is not yet available.

References

Cai Y, Xie Y, Li J. Glandless seed and glanded plant research in cotton: a review. Agronomy for Sustainable Development. 2010;30:181–190.

Bertrand JA, Sudduth TQ, Condon A, Jenkins TC, Calhoun MC. Nutrient content of whole cottonseed. Journal Dairy Science. 2005;88:1470–1477.

Carter D. Pest lepidoptera of Europe with special reference to the British Isles. Junk Publishers, Dordrecht; 1984.

El-Sheikh ES, El-Saleh MA, Aioub AA, Desuky WM. Toxic effects of neonicotinoid insecticides on a field strain of cotton leafworm, Spodoptera littoralis. Asian J Biol Sci. 2018;11:179–185.

Ahmed ME, Samah MH, Sahar EE. Differential Effects of Some Insect Growth Regulators on the Reproductive Potential of Lepidopteran Pest, Spodoptera littoralis. J. Bas. & Environ. Sci. 2019;6:257–266.

Aydin MH, Gürkan MO. The efficacy of spinosad on different strains of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). Turk J Biol. 2006;30:5–9.

Ishaaya I, Yablonski S, Horowitz AR. Comparative toxicity of two ecdystroids, RH-2485 and RH-5992 on susceptible and pyrethroid resistant strains of the Egyption cotton leafworm, Spodoptera littoralis. Phytoparasit. 1995;23:139–145.

Pavunraj M, Baskar K, Paulkumar K, Janarthanan S, Rajendran P. Antifeedant activity of crude extracts and fractions isolated from Catharanthus roseus leaf against spotted bollworm, Earias vittella. Phytoparasitica. 2016;44, 419–422.

Gill HK, Garg H. Pesticides: environmental impacts and management strategies. In: "PesticidesToxic Aspects". InTech; 2014.

Biondi A, Mommaerts V, Smagghe G, Viñuela E, Zappalà L, Desneux N. The non-target impact of spinosyns on beneficial arthropods. Pest Management Science. 2012;68:1523–1536.

Martínez LC, Plata-Rueda A, Gonçalves WG, Penha AF, Freire A, Zanuncio JC, Bozdoğan H, Serrão JE. Toxicity and cytotoxicity of the insecticide imidacloprid in the midgut of the predatory bug. Podisus nigrispinus. Ecotox. Environ. Safety. 2019;167, 69–75.

Sparks TC, Thompson GD, Kirst HA, Hertlein MB, Mynderse JS, Turner JR, Worden TV. Biological activity of the Spinosyns, new fermentation derived insect control agents for Tobacco budworm (Loepidoptera: Noctuidae) larvae. J. Econ. Entomol. 1998;91:1277-1283.

Thompson GD, Dutton R, Sparks TC. Spinosad, a case study: an example from a natural products discovery programme. Pest Management Science. 2000;56:696-702.

Salgado VL, Sparks TC. The spinosyns chemistry, biochemistry, mode of action, and resistance. In: Gilbert, L.J., Iatrou, K., Gill, S.S. (Eds.), Comprehensive Molecular Insect Science. Elsevier, Oxford. 2005;137-173.

Osorio A, Martinez AM, Schneider MI, Diaz O, Corrales JL, Aviles MC. Monitoring of beet armyworm resistance to spinosad and methoxyfenozide in Mexico. Pest Management Science. 2008; 64:1001-1007.

Williams T, Valle J, Vinuela E. Is the naturally-derived insecticide Spinosad® compatible with insect natural enemies? Biocontrol. Sci. Technol. 2003;13:459–475.

Pineda S, Budia F, Schneider MI. Gobbi A, Viñuela E, Valle J, Del EP. Effect of two biorational insecticides, spinosad and methoxyfenozide, on Spodoptera littoralis (Lepidoptera: Noctuidae) under laboratory conditions J. Econ. Entomol. 2004;97:1906–1911.

Osorio A, Martínez AM, Schneider MI, Díaz O, Corrales JL, Avilés MC, Smagghe G, Pineda S. Monitoring of beet armyworm resistance to spinosad and methoxyfenozide in Mexico. Pest Manag Sci. 2008;64:1001-1007.

Yanagi M, Tsukamoto Y, Watanabe T, Kawagishi A. Development of a novel lepidopteran insect control agent, chromafenozide. J. Pestic Sci. 2006;31:163-164.

Schneider MI, Smagghe G, Pineda S, Viñuela E. The ecological impact of four IGR insecticides in adults of Hyposoter didymator (Hym., Ichneumonidae). Pharmacokinetics approach. Ecotoxicology. 2008;17:181-188.

Pavela R. Effectiveness of some botanical insecticides against Spodoptera littoralis Boisduval (Lepidoptera: Noctudiae), Myzus persicae Sulzer (Hemiptera: Aphididae) and Tetranychus urticae Koch (Acari: Tetranychidae). Plant Protection Science. 2009;45:161-167.

El-Defrawi ME, Toppozada A, Mansour N, Zeid M. Toxicological studies on Egyptian cotton leafworm Prodenia litura (F.). I: Suceptiblity of different larval instars to insecticides. J. Econ. Entomol. 1964; 57(4):591- 593.

Abo El-Ghar GES, Radwan HAS, El-Bermawy ZA, Zidan LTM. Histopathological effects of abamectin, thuringiensis and diflubenzuron on the midgut of Spodoptera littoralis (Lepidoptera: Noctuidae) larvae. Bull. Ent. Soc. Egy. 1994;21:41-52.

Abbott WS. A method for computing the effectiveness of an insecticide. J. Econ. Entomol. 1925;18 (2): 256-267.

Finney DJ. Probit analysis: a statistical treatment of the sigmoid response curve. P. 33. Cambridge Univ. Press, London. 1971;33.

Junqueira LC, Carneiro J. Methods of study in “Basic Histology”. Land Medical Pub. 1980;1-18.

Suvarna KS, Layton C, Bancroft JD. Bancroft’s theory and practice of histological techniques, 8th ed. Elsevier Health Sciences; 2018.

Duncan DB. Multiple range and multiple F-test. Boimetrics. 1955;11(1):1- 24.

Mohamed AIA, Sobhy AST, Farouk AA, Samir HMM. The Effects of Selected Host Plants on the Efficacy of Spinosad Pesticide on Cotton Leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) Under Laboratory Conditions. Advances in Environmental Biology. 2015;9(3):372-375.

Marwa A. Moussa1, Zahia k. Moustafa, Hanan F. Abdel-Hafez, Abdel-Aziz A. Khidr. The Role of Mint Oil in Enhancing Spinosad Toxicity against the Cotton Leaf worm, Spodoptera littoralis in Relation to some Enzymes Activity, Egypt. Acad. J. Biolog. Sci. 2019;12(5):19-27.

El-Sheikh A. Biological, biochemical and histological effects of spinosad, Bacillus thuringiensis var. kurstaki and cypermethrin on the Cotton leafworm, Spodoptera littoralis (Boisd.). Egyptian Society of Biological Sciences. 2019;18.

Seham MI. Joint Toxic Action of Spinosad with Fenpropathrin and Chlorpyrifos and its Latent Effect on Different Egyptian Field Populations of Spodoptera littoralis, Asian J. Biol. Sci. 2020;13.

Ahmad MM, Arif I. Resistance of beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) to endosulfan, organophosphorus and pyrethroid insecticides in Pakistan. Crop Protection. 2010;29:1428- 1433.

Ibrahim EN. Physiological effects of certain bioagents and an insect growth regulator on the cotton leafworm Spodoptera littoralis (Boisd.) (Noctuidae: Lepidoptera). Unpublished M.Sc. Thesis, Fac. Agri., Ain Shams Univ., Egypt, Depart., Plant protect., Egypt. 2008;115.

Saleh HA, Soheir FA, El-Gably AR, Yacoub Sh. S, Khorchid AM. Biological and Histological Effects of Certain Insecticides on Spodoptera littoralis (Bosid). J. of Plant Protection and Pathology, Mansoura Univ. 2021;12(2):111 – 115.

Abdel-Aal AE, Abdel-wahab IS. Ovicidal activity and latent effects of lufenuron and spinosad on the cotton leafworm, Spodoptera littoralis. J. Agric. Sci. Mansoura Univ., 2007;32(6):4797 - 4806.

Saleh TA, Ahmed KS, Ismail EH. Ultrastructural Changes of the Cotton Leaf Worm, Spodoptera littoralis (Boisd.) Ovaries Induced by the two IGRs; Diflubenzuron and Chromafenozide. Egypt. Acad. J. Biolog. Sci. 2018;11(3):19-34.