Exploring the Role of Antioxidant Enzyme Activities in Reducing Oxidative Stress in the Earthworm Pheretima posthuma Exposed to Heavy Metal Contaminated Soil

Full Article - PDF

Published: 2023-03-11

Page: 161-166

S. S. Jadhav *

Department of Zoology, Amolak Science College, Kada, Tq. Ashti, Dist. Beed, 414202, India.

*Author to whom correspondence should be addressed.


The present study of 96 hrs was carried out to analyze the toxic effects of heavy metal lead nitrate (1.69 and 1.27 ppm) on anti-oxidative enzymes viz. Superoxide Dismutase, Catalase and Glutathione-S-transferase in Pheretima posthuma. The worms for this observation were exposed to different concentrations of heavy meals viz. The lower and higher concentration calculated 1.69 and 1.27 ppm dry soil for lead nitrate for 5 days of exposure. A dose and time dependent increase in activities of these enzymes was observed. Lead nitrate was found to be more toxic at its highest concentration.

Keywords: Anti-oxidative enzymes, super-oxide dismutase, catalase, glutathione-S-transferase, Pheretima posthuma

How to Cite

Jadhav, S. S. (2023). Exploring the Role of Antioxidant Enzyme Activities in Reducing Oxidative Stress in the Earthworm Pheretima posthuma Exposed to Heavy Metal Contaminated Soil. Asian Journal of Advances in Research, 6(1), 161–166. Retrieved from https://mbimph.com/index.php/AJOAIR/article/view/3412


Download data is not yet available.


Holleman AF, Wiberg E. Lehebuch du Anoranischen chemie. Water de Gruyter, Berlin. 1985;868.

Roubalová R, Dvořák J, Procházková P, Elhottová D, Rossmann P, Škanta F. The effect of dibenzo-p-dioxinand dibenzofuran-contaminated soil on the earthworm Eisenia andrei. Environmental Pollution. 2014;193:22-28.

Lanno R, Wells J, Conder J, Bradham K, Basta N. The bioavailability of chemicals in soil for earthworms. Ecotoxicology and Environmental Safety. 2004;57:39-47.

Scott MD, Lubin BH, Zuo L, Kuypers FA. Erythrocyte defense against hydrogen peroxide: preeminent importance of catalase. The Journal of laboratory and clinical medicine. 1991;118(1):7-16.

Edwards PJ, Coulson JM. Choice of earthworm species for laboratory tests. Ecotoxicology of earthworms. 1992;36-43.

Dash M C and Patra UC Density, biomass and energy budgetofa tropical earthworm population from a grassland site in Orissa, India; Rev. Ecol. Bioi. Sol. 1977;14461-471.

Panda S, Sahu SK. Acute toxicity assessment of three pesticide to the earthworm D.willisi J. Ecotoxicol. Environ. Monit. 2002;12(3):215-223.

Finney DJ. Probit Analysis. 3rd Edition, Cambridge University Press, Cambridge; 1971.

Marklund, S. and Marklund, G. Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase. European Journal of Biochemistry. 1974;47:469-474.

Chance B, Machly AC. Assay of catalases and peroxidises. Methods in Enzymology. 1955;2:764-775.

Habig WH, Pabst MJ, Jacoby NB. Glutathione-s-tranferase, the first enzymatic step in mecapatric acid formation. J. Bio.chem. 1974;249:7130-7139.

Shweta Sharma, RK Gupta, Dharambir Singh, Mahima and Anshu: Reported Super oxide dismutase, Catalase and glutathione peroxidase activity in heavy metals contaminated earthworm, Eisenia fetida Journal of Entomology and Zoology Studies. 2020;8(6):1909-1913.

Ravi Kiran T. h. K. Aruna antioxidant enzyme activities and markers of oxidative stress in the life cycle of earthworm, Eudrilus eugeniae. Italian Journal of Zoology. 2010;77(2):144–148.

Stadtman ER. Protein oxidation and aging. Science. 1992;257:1220–1224.

Stenersen J, Guthenberg C, Mannervik B. Glutathione-Stransferases in earthworms Lumbricidae). Biochemistry. 1979;181:47–50.

Gaete H, Hidalgo ME, Neaman A, Ávila G. Evaluación de la toxicidad de cobre en suelos a través de biomarcadores de estrés oxidativo en Eisenia foetida. Química Nova. 2010;33(3):566-570.

Zhang Q, Zhang B, Wang C. Ecotoxicological effects on the earthworm Eisenia fetida following exposure to soil contaminated with imidacloprid. Environmental Science and Pollution Research. 2014;21(21):12345-1235.

Cao Q, Steinman AD, Yao L, Xie L. Toxicological and biochemical responses of the earthworm Eisenia fetida to cyanobacteria toxins. Scientific reports. 2017;7(1):1-9.

Costa V, Amorim MA, Reis E, Quintanilha A, MoradasFerreira P. Mitochondrial superoxide dismutase is essential for ethanol tolerance of Saccharomyces cerevisiae in the post-diauxic phase. Microbiology. 1997;143(5):1649-1656.