STUDIES ON In-vitro EVALUATION OF ANTIMICROBIAL ACTIVITY OF SILKWORM COCOON EXTRACTS

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Published: 2022-01-29

DOI: 10.56557/upjoz/2022/v43i22900

Page: 43-49


. DEEPALI *

Sri Sai University, Palampur, H.P., India.

MEENAKSHI SHARMA

Kehloor Biosciences and Research Centre, Ghumarwin, Bilaspur, H.P., India.

ANEESH KUMAR

Kehloor Biosciences and Research Centre, Ghumarwin, Bilaspur, H.P., India.

AMIT KUMAR

Kehloor Biosciences and Research Centre, Ghumarwin, Bilaspur, H.P., India.

VIKESH KUMAR BHATIA

Kehloor Biosciences and Research Centre, Ghumarwin, Bilaspur, H.P., India.

PRIYANKA KUMARI

Department of Zoology, Sri Sai University Palampur, H.P, India.

*Author to whom correspondence should be addressed.


Abstract

Silk from the domesticated Bombyx mori cocoons is composed of adhesive glue (sericin) and fiber (fibroins) proteins which provide a physical barrier to protect pupa. The recovery and reuse of sericin not only minimizes environmental issues but also has high scientific and commercial value. The antimicrobial properties of sericin allow its application for drug delivery and cosmetics. The aim of the present study was to elucidate the antimicrobial efficacy persisted in the cocoons of Bombyx mori. The silkworm cocoon shell extracts were prepared, and their in vitro antimicrobial susceptibility testing was performed using disc diffusion method against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae. The cocoon shell extracts demonstrated significant antimicrobial activity. It was observed that all the bacterial strains were resistant against cepodoxime. These results unwrap the opportunity to develop a natural antibiotic based on sericin that may be used for the treatment of drug resistant bacteria. However further studies are still required to demonstrate the other beneficial effects of sericin.

Keywords: Antimicrobial, sericin, Bombyx mori, cocoon


How to Cite

DEEPALI, ., SHARMA, M., KUMAR, A., KUMAR, A., BHATIA, V. K., & KUMARI, P. (2022). STUDIES ON In-vitro EVALUATION OF ANTIMICROBIAL ACTIVITY OF SILKWORM COCOON EXTRACTS. UTTAR PRADESH JOURNAL OF ZOOLOGY, 43(2), 43–49. https://doi.org/10.56557/upjoz/2022/v43i22900

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References

Soumya M, Harinatha Reddy A, Nageswari G, Venkatappa B. Silkworm (Bombyx mori) and its constituents: A fascinating insect in science and research Journal of Entomology and Zoology Studies 2017;5(5):1701-1705.

Ramesbabu, Ramakrishna K.S, Harishkumarreddy Y, lakshmi G, Naidu, S N.V. Metabolic molecularMechanism in silkworm larvae during viral infection: A review, African journal of Biotechnology. 2009;8:899- 907.

Guo X, Dong Z, Zhang Y, Li Y, Liu H, Xia Q, et al. Proteins in the Cocoon of Silkworm Inhibit the Growth of Beauveria bassiana. PLoS ONE. 2016;11(3):e0151764.

Kunz RI, Brancalhão RMC, de Fátima Chasko Ribeiro L, Natali MRM. Review Article Silkworm Sericin: Properties and Biomedical Applications. 2016;1-19.

Sarovart S, Sudatis B, Meesilpa P, Grady BP, Magaraphan R. The use of sericin as an antioxidant and antimicrobial for polluted air treatment. Rev Adv Mater Sci. 2003;5(3):193-198.

Asakura T, Yao J, Yang M, Zhu Z, Hirose H. Structure of the spinning apparatus of a wild silkworm Samia cynthia ricini and molecular dynamics calculation on the structural change of the silk fibroin. Polymer. 2007;48:2064–2070.

Pandiarajan J, Cathrin BP, Pratheep T, and Krishnan M. Defense role of the cocoon in the silk worm Bombyx mori L. Rapid Commun. Mass Spectrom. 2011;25:3203–3206.

Leucht S, Corves C, Arbter D, Engel RR, Li C, Davis JM. Second-generation versus first-generation antipsychotic drugs for schizophrenia: A meta-analysis. The Lancet. 2009;373(9657):31-41.

Giske CG, Monnet DL, Cars O, Carmeli Y. Clinical and economic impact of common multidrug-resistant gram-negative bacilli. Antimicrobial Agents and Chemotherapy. 2008;52(3):813-821.

Senakoon W, Nuchadomrong S, Sirimungkararat S, Senawong T, Kitikoon P. Antibacterial action of eri (samia ricini) sericin against Escherichia coli and Staphylococcus aureus. Asian Journal of Food and Agriculture Industry. 2009;S222–S228.

Pak. J. Biochem. Mol. Biol. 2015;48(1):18-21 Antibacterial activity of natural honey against antibiotic-resistant bacteria Aurongzeb M, Kamran Azim M.

Mohammedaman Mama, Teklu Teshome, Jafer Detamo. Antibacterial Activity of Honey against Methicillin-Resistant Staphylococcus aureus: A Laboratory-Based Experimental Study. International Journal of Microbiology. 2019;1-9.

Kundu SC, Dash BC, Dash R, Kaplan DL. Natural protective glue protein, sericin bio engineered by silkworms: Potential for biomedical and biotechnological applications. Progress in Polymer Science. 2008;33:998-1012.

Rochaa LKH, Favaroa LIL, Riosa AC, Silvaa EC, Silvaa WF et al. Sericin from Bombyx mori cocoons. Part I: Extraction and physicochemical-biological characterization for biopharmaceutical applications. Process Biochemistry. 2017;61:163-177.

Pandiarajan, Jeyaraj, et al. Defense role of the cocoon in the silk worm Bombyx mori L." Rapid Communications in Mass Spectrometry. 2011;25(21):3203-3206.

Wright S, Goodacre S. Evidence for antimicrobial activity associated with common house spider silk. BMC Research Notes. 2012;5:326.

Rajendran R, Balakumar C, Sivakumar R, Amruta T, Devaki N. Extraction and application of natural silk protein sericin from Bombyx mori as antimicrobial finish for cotton fabrics, The Journal of The Textile Institute. 2012;103(4):458-462.

El-Seedi HR, El-Said AM, Khalifa SA, Goransson U, Bohlin L, Borg-Karlson AK, Verpoorte R. Biosynthesis, natural sources, dietary intake, pharmacokinetic properties, and biological activities of hydroxycinnamic acids. Journal of Agricultural and Food Chemistry. 2012;60(44):10877-10895.

Seves A, Romanò M, Maifreni T, Sora S, Ciferri O. The microbial degradation of silk: a laboratory investigation. International Biodeterioration & Biodegredation. 1999;42:203-211.

Singh M, Kaur M, Silakari O. Flavones: An important scaffold for medicinal chemistry. European Journal of Medicinal Chemistry. 2014;84:206-239.

Kunz RI. Propriedades, potenciais aplicações biomédicas e efeitos da sericina de Bombyx mori sobre a morfofisiologia de camundongos obesos por dieta hiperlipídica; 2017.

Fymat AL. Antibiotics and antibiotic resistance. Biomed J Sci & Tech Res. 2017; 1(1):1-16.