Published: 2022-04-20

DOI: 10.56557/upjoz/2022/v43i62974

Page: 19-25


Laboratory of Improvement and Development of Animal and Plant Production, Ferhat Abbas University, Setif, Algeria.


Laboratory of Terrestrial and Aquatic Ecosystems, Department of Biology, Mohamed Cherif Messaadia University, -Souk-Ahras 41000, Algeria.


Excellence Laboratory of Animal Applied Biology, Department of Biology, Faculty of Sciences, Badji Mokhtar, University, Annaba 23000, Algeria.

*Author to whom correspondence should be addressed.


The domestic mosquito Culex pipiens and Culiseta longiareolata are considered to be the most important mosquito species in Algeria, due to their large geographical distribution and abundance. Instead of causing environmental nuisance, they transmit diseases to the animals. The mosquito control, using conventional insecticides becomes harmful to human and to the environment and in addition mosquitoes have developed a resistance against these products. These reasons have encouraged scientists to propose new eco-friendly and effective alternatives to control insects. Plants extracts have showed a promising agents for pest treatments.  In order to test a botanical larvicide against mosquito, extracts were prepared from the leaves of Marrubium vulgare, Laurus nobilis, Eucalyptus globulus, and Myrtus communis and the extractions were done with methanol, using a Soxhlet extractor. Bioassay was carried out, using different concentrations against the fourth mosquito larvae of the C. longiareolata.  The toxicity of the tested extracts was evaluated and the lethal concentrations, LC50 and LC90) values were estimated. The obtained results indicated a sensitivity of Cs. longiareolata larvae to the four plants species, with dose-response relationship mortality. The lethal concentrations were calculated with their confidence limits and their values were for LC50 of 6.13, 7.03, 9.60 and 17.84g/l, while the LC90’s were 22, 37.19, 75.69 and 92.76g/l for M. vulgare, L. nobilis, E. globulus, and Myrtus communis respectively. These toxicological assays, using these autochthon plant extracts could be developed as an alternative compound for mosquito control programme.

Keywords: Plant extracts, Culiseta longiareolata, insecticide, mosquito control

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Tandina F, Doumbo O, Yaro AS, Traoré SF, Parola P, Robert V. Mosquitoes (Diptera: Culicidae) and mosquito-borne diseases in Mali, West Africa. Para Vect. 2018;11:467–472.


Fernandes RS, Bersot MI, Castro MG, Telleria EL, Ferreira-de-Brito A, Raphael LM, Bonaldo MC, Lourenço-de-Oliveira R. Low vector competence in sylvatic mosquitoes limits Zika virus to initiate an enzootic cycle in South America. Sci Rep. 2019;9(1):20151–20158.

DOI: 10.1038/s41598-019-56669-4.

World Health Organisation. Report of the WHO informal consultation on the evaluation on the testing of insecticides, CTD/WHO PES/ICAU- ER- /96.1. Geneva. WHO. 1996;69.

Aissaoui L, Boudjelida H. Diversity and distribution of Culicinae fauna in Tebessa district (North-.East of Algeria). Int J mosq Res. 2017;4(1):07–12.

Arroussi DER, Bouaziz A, Boudjelida H. Mosquito survey reveals the first record of Aedes (Diptera: Culicidae) species in urban area, Annaba district, Northeastern Algeria. Pol J Entomol. 2020;90(1):14–26.

DOI: 10.5604/01.3001.0014.8065.

Isra D, Ibáñez-Justicia A, De Wolf K, Smitz N, Schneider S, Stroo A, et al. First Detections of Culiseta longiareolata (Diptera: Culicidae) in Belgium and the Netherlands. J of Med Entomol. 2021;127:654–661.


Ombugadu, A, Echor BO, Jibril AB, Angbalaga GA, Lapang MP, Micah EM, et al. Impact of Parasites in Captive Birds: A Review. Current Res Envr Biodiver. 2022;01: 352–359.

Hurd H, Hogg JC. Interactions between blood feeding, fecundity and infection in mosquitoes. Parasitol Today. 1995;11:411–416.

Van Pletzen R, Van der Linde TC. Laboratory Studies on the Biology of Culiseta longiareolata (Macquart) (Diptera: Culicidae). Bull Entomol Res. 1981;71:71–79.

Seidel B, Nowotny N, Duh D, Indra A, Hufnagl P, Allerberger, F. First records of the thermophilic mosquito Cs. longiareolata (Macquart, 1838) in Austria, 2012, and in Slovenia. J Eur Mosq Control Assoc. 2013;31: 17–20.

Khaligh FG, Naghian A, Soltanbeiglou S, Gholizadeh S. Autogeny in Culiseta longiareolata (Culicidae: Diptera) mosquitoes in laboratory conditions in Iran. BMC Res Notes. 2020; 13:81–89.

Naqqash, MN, Gökçe A, Bakhsh A, Salim M. Insecticide resistance and its molecular basis in urban insect pests. Parasitol Res. 2016;115: 1363–1373.

Pavela R. Essential oils for the development of eco-friendly mosquito larvicides: A review. Indust Crop Prod. 2015;76:174–87.

Javadi Khederi S, Khanjani M, Gholami M, de Lillo E. Resistance of grapevine to the erineum strain of Colomerus vitis (Acari: Eriophyidae) in western Iran and its correlation with plant features. Exper App Acarol. 2014;63:15–35.

Javadi Khederi S, Khoobdel M, Khanjani M, Hosseininia A, Sorkhe Dizaji BS, Hosseini SM, Sobati H. Insecticidal effects of essential oils from two medicinal plants against Aleuroclava jasmini (Hemiptera: Aleyrodidae. J. Crop Prot. 2019; 8 (1): 57–66.

World Health Organisation. Report of the eight WHOPES working group meeting. Review of: Novaluron 10% EC. WHO/CDS/WHOPES/ 2005.10.

Nabti I, Bounechada M. Mosquito biodiversity in Setif region (Algerian High Plains), density and species distribution across climate zones. Faun entomol. 2020;73 :1–14.

Irrusappan H, Nisha M. Larvicidal activity of selected plant extracts and their combination against the mosquito vectors Culex quinquefasciatus and Aedes aegypti. Env Sci Poll Res. 2018;25:9176–9185.

Abbott WB. A method for computing the effectiveness of an insecticide. J Eco Entomol. 1925;18:265–267.

Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal plants: Past history and future perspective. J. Herbmed Pharmacol. 2018;7:1–7.

Dadaliogÿlu I, Akdemir Evrendilek G. Chemical Compositions and Antibacterial Effects of Essential Oils of Turkish Oregano (Origanum minutiflorum), Bay Laurel (Laurus nobilis), Spanish Lavender (Lavandula stoechas L.),and Fennel (Foeniculum vulgare) on Common Foodborne Pathogens. J. Agric. Food Chem. 2004;52:8255–8260.

Raho B. Antimicrobial activity of Eucalyptus globulus oils. Available from; In book: Antimicrobial research: Novel bioknowledge and educational programs, Editors: A. Méndez-Vilas; 2017.

Ibrahim M, Agour A, Lyoussi B, Derwich EH. In Vitro Antibacterial Properties and Antioxidant Activity of Essential Oils from Marrubium vulgare L. Trop J Nat Prod Res. 2021;5(4):661–667.

Mohamadi Y, Lograda T, Ramdani M, Figueredo G, Chalard P. Chemical composition and antimicrobial activity of Myrtus communis essential oils from Algeria. Biodivers J Biol Divers. 2021;22(2):933–946.


Mohankumar TK, Shivanna KS, Achuttan VV. Screening of Methanolic Plant Extracts against Larvae of Aedes aegypti and Anopheles stephensi in Mysore. J Arthropod Borne Dis. 2016;10(3):303–14.

Thongwat D, Chokchaisiri R, Ganranoo L, Bunchu N. Larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri Gagnep against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquito vectors. Asian Pac J Trop Biomed. 2018;8:273–8.

Djeghader NEH, Aïssaoui L, Amira K, Boudjelida H. Toxicity evaluation and effects on the development of a plant extract, the Saponin, on the domestic mosquito, Culex pipiens. Int J Mosq Res. 2018;5(1):01–05.

Djeddar H, Boudjelida H, Arroussi DER. New alternative for culicidian fauna control using Borago officinalis and Drimia maritima plant extracts. Biodiversitas. 2021;22(12):5688–5694.

Rouari L, H. Gouzi1 M. Ghermaoui1 F, Benaceur A, Kemassi B, Merabti I. et al. First study of larvicidal activity of Algerian Oudneya africana extracts against Culex pipiens larvae. Ukrainian J Ecol. 2022; 12(1): 65–70.

DOI: 10.15421/2022-337.

Amira Khedidja, Touahria Chouaib, Nour El-Houda Djeghader and Hamid Boudjelida.Laboratory study of the larvicidal efficacy of a local plant Hertia cheirifolia against the most abundant mosquito species, in Algeria J Entomol Zool Stud. 2018;6(1): 258–262.

Mekhlif Atallah F, Mohammad M J. Larvicidal potentials of four medicinal plant extracts on mosquito vector, Culex pipiens molestus (Diptera: Culicidae) Int J Mosq Res. 2021; 8(4): 01–05.

Nabti I, Bounechada M. Larvicidal Activities of Essential Oils Extracted from Five Algerian Medicinal Plants against Culiseta longiareolata Macquart. Larvae (Diptera: Culicidae). Eur J Biol. 2019;78(2):132–138.

Abutaha N, AL-Mekhlafi FA, AL-Keridis lA, Farooq M, Nasr FA, AL-Wadaan M. Larvicidal potency of selected xerophytic plant extracts on Culex pipiens (Diptera: Culicidae). Entomol Res. 2018; 48: 362–371.