THE MUCUS OF Helix aspersa AS AN ANTI-INFLAMMATORY AND ANTIBACTERIAL TREATMENT TOWARDS MULTIDRUG-RESISTANT BACTERIA FROM INFECTED WOUNDS

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Published: 2022-09-03

DOI: 10.56557/upjoz/2022/v43i163139

Page: 28-36


NESMA MERAH

Laboratory of Biochemistry and Environmental Toxicology, Department of Biochemistry, Faculty of Sciences, Badji Mokhtar University, Annaba, Algeria.

MABROUKA BOUACHA *

Laboratory of Biochemistry and Environmental Toxicology, Department of Biochemistry, Faculty of Sciences, Badji Mokhtar University, Annaba, Algeria.

SANA BESNACI

Laboratory of Cellular Toxicology, Department of Biology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria.

MINA KOUCH

Department of Biochemistry, Faculty of Sciences, University Badji Mokhtar, Annaba, Algeria.

*Author to whom correspondence should be addressed.


Abstract

The mucus of snails is known for its high content of bioactive substances, which can be effective in the treatment of infected wounds. The objective of this study is to evaluate the antibacterial and anti-inflammatory activity of three extracts of Helix aspersa mucus.

The evaluation of the antibacterial activity of mucus extracts was carried out against three Gram-negative bacteria isolated from infected wounds using the well diffusion method, the microdilution for the minimum inhibitory concentrations (MIC) determination, and minimum bactericidal concentrations (MBC) determination. The anti-inflammatory activity was carried out using albumin denaturation inhibition and the human red blood cell membrane stabilization methods. The results obtained showed that snail mucus extracts possess an inhibitory effect on the growth and viability of Gram-negative bacteria. The inhibitory diameters ranged between 09.33±1.11 and 12.00±0.77 mm, the MIC was 25 % (v/v), and the MBC ranged between 25 and 50 % (v/v). The minimum bactericidal concentrations/minimum inhibitory concentrations (MBC/MIC) ratios indicate that snail mucus possesses a bacterial effect on Gram-negative bacteria. Furthermore, snail mucus possesses an anti-inflammatory effect through the inhibition of protein denaturation and membrane stabilization.

The results obtained from this study encourage the use of Helix aspersa mucus in clinical practice as an antibacterial and anti-inflammatory agent in the treatment of infected wounds.

Keywords: Antibacterial effect, anti-inflammatory effect, Helix aspersa, infected wounds; mucus


How to Cite

MERAH, N., BOUACHA, M., BESNACI, S., & KOUCH, M. (2022). THE MUCUS OF Helix aspersa AS AN ANTI-INFLAMMATORY AND ANTIBACTERIAL TREATMENT TOWARDS MULTIDRUG-RESISTANT BACTERIA FROM INFECTED WOUNDS. UTTAR PRADESH JOURNAL OF ZOOLOGY, 43(16), 28–36. https://doi.org/10.56557/upjoz/2022/v43i163139

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References

Brandt AM, Gardner M. The golden age of medicine? Med Twent Century. Taylor & Francis. 2020;21–37.

Adamu HI, Inah MB. A Review on the Use of Honey in the Treatment of Wound Infection. Asian Food Sci J. 2021;51–9.

Chelkeba L, Melaku T, Mega TA. Gram-negative bacteria isolates and their antibiotic-resistance patterns in patients with wound infection in Ethiopia: A systematic review and meta-analysis. Infect Drug Resist. Dove Press; 2021;14:277.

Mahmud F, Roy R, Mohamed MF, Aboonabi A, Moric M, Ghoreishi K, et al. Therapeutic evaluation of immunomodulators in reducing surgical wound infection. FASEB J. Wiley Online Library; 2022;36:e22090.

Bouacha M, Besnaci S, Boudiar I, Al-kafaween MA. Impact of Storage on Honey Antibacterial and Antioxidant Activities and their Correlation with Polyphenolic Content. 2022;6:34–9.

Hassan MA, Abd El-Aziz S, Elbadry HM, Samy A, Tamer TM. Prevalence, antimicrobial resistance profile, and characterization of multi-drug resistant bacteria from various infected wounds in North Egypt. Saudi J Biol Sci. Elsevier; 2022;

Cilia G, Fratini F. Antimicrobial properties of terrestrial snail and slug mucus. J Complement Integr Med. De Gruyter; 2018;15.

Harti AS, Murharyati A, Sulisetyawati SD, Oktariani M. The effectiveness of snail mucus (Achatina fulica) and chitosan toward limfosit proliferation in vitro. Asian J Pharm Clin Res. 2018;11:85–8.

Liudmyla K, Olena C, Nadiia S. Chemical properties of Helix aspersa mucus as a component of cosmetics and pharmaceutical products. Mater Today Proc [Internet]. 2022 [cited 2022 May 17]; Available from: https://www.sciencedirect.com/science/article/pii/S221478532200846X

Zhong J, Wang W, Yang X, Yan X, Liu R. A novel cysteine-rich antimicrobial peptide from the mucus of the snail of Achatina fulica. Peptides. Elsevier; 2013;39:1–5.

Belouhova M, Daskalova E, Yotinov I, Topalova Y, Velkova L, Dolashki A, et al. Microbial diversity of garden snail mucus. Microbiology Open. 2022;11:e1263.

Gabriel UI, Mirela S, Ionel J. Quantification of mucoproteins (glycoproteins) from snails mucus, Helix aspersa and Helix Pomatia. J Agroaliment Process Technol. 2011;17: 410–3.

Topalova Y, Belouhova M, Velkova L, Dolashki A, Zheleva N, Daskalova E, et al. Effect and Mechanisms of Antibacterial Peptide Fraction from Mucus of C. aspersum against Escherichia coli NBIMCC 8785. Biomedicines. Multidisciplinary Digital Publishing Institute; 2022;10:672.

Iguchi SM, Aikawa T, Matsumoto JJ. Antibacterial activity of snail mucus mucin. Comp Biochem Physiol A Physiol. Elsevier; 1982;72:571–4.

Razdan K, Garcia-Lara J, Sinha VR, Singh KK. Pharmaceutical strategies for the treatment of bacterial biofilms in chronic wounds. Drug Discov Today [Internet]. 2022 [cited 2022 May 17];

Available:https://www.sciencedirect.com/science/article/pii/S1359644622001684

Bouacha M, Besnaci S, Boudiar I, Al-KAFAWEEN MA. Screening of the antibacterial and antibiofilm effect of multifloral honey against multidrug-resistant Pseudomonas aeruginosa. 2022;67:11.

Cunrath O, Meinel DM, Maturana P, Fanous J, Buyck JM, Saint Auguste P, et al. Quantitative contribution of efflux to multi-drug resistance of clinical Escherichia coli and Pseudomonas aeruginosa strains. EBioMedicine. Elsevier; 2019;41:479–87.

Alam MM, Islam MN, Hawlader MDH, Ahmed S, Wahab A, Islam M, et al. Prevalence of multidrug resistance bacterial isolates from infected wound patients in Dhaka, Bangladesh: a cross-sectional study. Int J Surg Open. Elsevier; 2021;28:56–62.

Kabanangi F, Nkuwi EJ, Manyahi J, Moyo S, Majigo M. High level of multidrug-resistant gram-negative pathogens causing burn wound infections in hospitalized children in dar es salaam, tanzania. Int J Microbiol. Hindawi; 2021;2021.

Pankok F, Taudien S, Dekker D, Thye T, Oppong K, Wiafe Akenten C, et al. Epidemiology of Plasmids in Escherichia coli and Klebsiella pneumoniae with Acquired Extended Spectrum Beta-Lactamase Genes Isolated from Chronic Wounds in Ghana. Antibiotics. Multidisciplinary Digital Publishing Institute. 2022;11:689.

Besnaci S, Bensoltane S, Braia FMH, Zerari L, Khadri S, Loucif H. Embryotoxicity evaluation of iron oxide Fe2O3 on land snails: Helix aspersa. J Entomol Zool Stud. 2016;4:317– 23.

Besnaci S, Bouanani N, Bouacha M, Babouri Y, Bensoltane S. An evaluation and comparative study of iron oxide effects in its nano and micron size on earth snails Helix aspersa. J Entomol Res. Malhotra Publishing House; 2022;46:217–22.

Ali SS, Morsy R, El-Zawawy NA, Fareed MF, Bedaiwy MY. Synthesized zinc peroxide nanoparticles (ZnO2-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds. Int J Nanomedicine. Dove Press; 2017;12:6059.

AL-Kafaween M, Hilmi A, Khan R, Bouacha M, Amonov M. Effect of Trigona honey on Escherichia coli cell culture growth: in vitro study. J Apitherapy. 2019;5:10.

Bouacha M, Ayed H, Grara N. Honey Bee as Alternative Medicine to Treat Eleven Multidrug-Resistant Bacteria Causing Urinary Tract Infection during Pregnancy. Sci Pharm. 2018;86:11.

Sharma P, Parthasarathi S, Patil N, Waskar M, Raut JS, Puranik M, et al. Assessing barriers for antimicrobial penetration in complex asymmetric bacterial membranes: A case study with thymol. Langmuir. ACS Publications; 2020;36:8800–14.

Vergalli J, Bodrenko IV, Masi M, Moynié L, Acosta-Gutierrez S, Naismith JH, et al. Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria. Nat Rev Microbiol. Nature Publishing Group; 2020;18:164–76.

Courvalin P. Predictable and unpredictable evolution of antibiotic resistance. J Intern Med. Wiley Online Library; 2008;264:4–16.

Tsoutsos D, Kakagia D, Tamparopoulos K. The efficacy of Helix aspersa Müller extract in the healing of partial thickness burns: a novel treatment for open burn management protocols. J Dermatol Treat. Taylor & Francis; 2009;20:219–22.

Dolashki A, Nissimova A, Daskalova E, Velkova L, Topalova Y, Hristova P, et al. Structure and antibacterial activity of isolated peptides from the mucus of garden snail Cornu aspersum. Bulg Chem Commun C. 2018;50:195–200.

El-Zawawy NA, Mona MM. Antimicrobial efficacy of Egyptian Eremina desertorum and Helix aspersa snail mucus with a novel approach to their anti-inflammatory and wound healing potencies. Sci Rep. Nature Publishing Group; 2021;11:1–11.

Rosanto YB, Hasan CY, Rahardjo R, Pangestiningsih TW. Effect of snail mucus on angiogenesis during wound healing. F1000Research. F1000 Research Limited London, UK; 2021;10:181.

Schultz GS, Sibbald RG, Falanga V, Ayello EA, Dowsett C, Harding K, et al. Wound bed preparation: a systematic approach to wound management. Wound Repair Regen. Wiley Online Library; 2003;11:S1– 28.

Edwards R, Harding KG. Bacteria and wound healing. Curr Opin Infect Dis. LWW; 2004;17:91–6.

Dinica RM, Sandu C, Dediu Botezatu AV, Cazanevscaia Busuioc A, Balanescu F, Ionica Mihaila MD, et al. Allantoin from Valuable Romanian Animal and Plant Sources with Promising Anti-Inflammatory Activity as a Nutricosmetic Ingredient. Sustainability. MDPI; 2021;13:10170.

Vadivu R, Lakshmi KS. In vitro and in vivo anti-inflammatory activity of leaves of Symplocos cochinchinensis (Lour) Moore ssp Laurina. Bangladesh J Pharmacol. 2008;3:121–4.

Pitt SJ, Graham MA, Dedi CG, Taylor-Harris PM, Gunn A. Antimicrobial properties of mucus from the brown garden snail Helix aspersa. Br J Biomed Sci. Taylor & Francis; 2015;72:174–81.

Bortolotti D, Trapella C, Bernardi T, Rizzo R. Antimicrobial properties of mucus from the brown garden snail Helix aspersa. Br J Biomed Sci. 2016;73:49–50.

Etim L, Aleruchi C, Obande G. Antibacterial properties of snail mucus on bacteria isolated from patients with wound infection. Br Microbiol Res J. 2016;11:1–9.

O’Neill AJ, Chopra I. Preclinical evaluation of novel antibacterial agents by microbiological and molecular techniques. Expert Opin Investig Drugs. 2004;13:1045– 63.

Dolashka P, Dolashki A, Velkova L, Stevanovic S, Molin L, Traldi P, et al. Bioactive compounds isolated from garden snails. J Biosci Biotechnol; 2015.

Franklin TJ, Snow GA. Penetrating the defences: How antimicrobial drugs reach their targets. Biochem Mol Biol Antimicrob Drug Action. Springer. 1998; 107–18.

Abiona JA, Akinduti A, Osinowo OA, Onagbesan OM. Comparative evaluation of inhibitory activity of epiphgram from albino and normal skinned giant African land snail (Archachatina marginata) against selected bacteria isolates. Ethiop J Environ Stud Manag. 2013;6:177– 81.