SECONDARY METABOLITES OF ANTAGONISTIC BACTERIAL STRAIN Streptomyces griseus KD1 EXERT ANTIBACTERIAL AND ANTICANCER ACTIVITY AGAINST BREAST CANCER MCF-7 CELLS

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Published: 2022-12-29

DOI: 10.56557/upjoz/2022/v43i243337

Page: 393-401


RAJASRI CELLADURAI

Department of Microbiology, Faculty of Science, Annamalai University, Annamalainagar, Chidambaram, Tamil Nadu-608002, India.

SUMATHI VADAMALAI *

Department of Microbiology, Faculty of Science, Annamalai University, Annamalainagar, Chidambaram, Tamil Nadu-608002, India.

*Author to whom correspondence should be addressed.


Abstract

Numerous types of bioactive compounds are produced in large quantities by marine microorganisms. In this work, the antagonistic marine bacterium strain Streptomyces griseus was isolated from soil samples taken from the beach into mangrove estuary region, and its unprocessed secondary metabolites were assessed for their potential antibacterial and anticancer properties. Using 16S rRNA sequencing study, the strong antagonistic bacterial isolate S. griseus was discovered. After being isolated from strain S. griseus using centrifugation and ethanol, the secondary metabolites were tested for antibacterial efficacy against bacterial pathogens of the respiratory tract using the agar well diffusion technique. This investigation indicated that the metabolic extract of the KD1 successfully reduced the development of clinical bacterial infections. Also confirmed concentration-dependent cytotoxicity against MCF-7 breast cancer cells. When metabolic extract of the KD1 was administered, it effectively inhibited the growth of MCF-7 cell colonies, increased lipid peroxidation, and lowered antioxidant activity. The findings demonstrated that the KD1 metabolic extract had potent antibacterial and anticancer activities. Therefore, with further clinical testing, it is possible to develop effective antibiotics and anticancer medications for breast cancer using the secondary metabolites of the S. griseus KD1 strain.

Keywords: Streptomyces griseus, MCF-7 cells, cytotoxicity, antibacterial, breast cancer


How to Cite

CELLADURAI, R., & VADAMALAI, S. (2022). SECONDARY METABOLITES OF ANTAGONISTIC BACTERIAL STRAIN Streptomyces griseus KD1 EXERT ANTIBACTERIAL AND ANTICANCER ACTIVITY AGAINST BREAST CANCER MCF-7 CELLS. UTTAR PRADESH JOURNAL OF ZOOLOGY, 43(24), 393–401. https://doi.org/10.56557/upjoz/2022/v43i243337

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