Published: 2022-12-29

DOI: 10.56557/upjoz/2022/v43i243337

Page: 393-401


Department of Microbiology, Faculty of Science, Annamalai University, Annamalainagar, Chidambaram, Tamil Nadu-608002, India.


Department of Microbiology, Faculty of Science, Annamalai University, Annamalainagar, Chidambaram, Tamil Nadu-608002, India.

*Author to whom correspondence should be addressed.


Numerous types of bioactive compounds are produced in large quantities by marine microorganisms. In this work, the antagonistic marine bacterium strain Streptomyces griseus was isolated from soil samples taken from the beach into mangrove estuary region, and its unprocessed secondary metabolites were assessed for their potential antibacterial and anticancer properties. Using 16S rRNA sequencing study, the strong antagonistic bacterial isolate S. griseus was discovered. After being isolated from strain S. griseus using centrifugation and ethanol, the secondary metabolites were tested for antibacterial efficacy against bacterial pathogens of the respiratory tract using the agar well diffusion technique. This investigation indicated that the metabolic extract of the KD1 successfully reduced the development of clinical bacterial infections. Also confirmed concentration-dependent cytotoxicity against MCF-7 breast cancer cells. When metabolic extract of the KD1 was administered, it effectively inhibited the growth of MCF-7 cell colonies, increased lipid peroxidation, and lowered antioxidant activity. The findings demonstrated that the KD1 metabolic extract had potent antibacterial and anticancer activities. Therefore, with further clinical testing, it is possible to develop effective antibiotics and anticancer medications for breast cancer using the secondary metabolites of the S. griseus KD1 strain.

Keywords: Streptomyces griseus, MCF-7 cells, cytotoxicity, antibacterial, breast cancer

How to Cite



Download data is not yet available.


Thatoi HN, Biswal AK. Mangroves of Orissa coast: floral diversity and conservation status. Special habitats and threatened plants of India. ENVIS Wild Life And Protected Area. 2008;11 (1):201–207.

Sahoo K, Dhal NK. (Potential microbial diversity in mangrove ecosystem, A review Indian Journal of Marine Sciences. 2009;38(2):249-256.

Shirling EB, Gottlieb D. Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study. Int J Syst Evol Microbiol. 1968a;38:69-189.

Shirling EB, Gottlieb D. Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. Int J Syst Evol Microbiol. 1968b;18:279- 392.

Alongi DM, Christoffersen P, Tirendi F. The influence of forest type on microbial-nutrient relationships in tropical mangrove sediments. J Exp Mar Biol Ecol. 1993;171:201–223.

Bull AT, Stach JEM, Ward AC, Goodfellow M. Marine actinobacteria: perspectives, challenges, future directions. Antonie van Leeuwenhoek. 2005;87:65–79.

Maldonado LA, Stach JEM, Pathom-aree W, Ward AC, Bull AT, Goodfellow M. Diversity of cultivable actinobacteria ingeographically widespreadmarine sediments. Antonie van Leeuwenhoek. 2005;87:1118.

Lam KS. Discovery of novel metabolites from marine actinomycetes. Curr Opin Microbiol. 2006;9:245L 251.

Lakshmipathy TD, Arun Prasad AS, Kannabiran K. Production of biosurfactant and heavy metal resistance activity of Streptomyces spp. Adv Biol Res. 2010;4(2):108–15.

Poosarla A, Venkata RL, Krishna MR. Isolation of potent antibiotic producing actinomycetes from marine sediments of Andaman and Nicobar Marine Islands. J Microbiol Antimicrob. 2013;5(1):6–12.

Delcour AH. Outer membrane permeability and antibiotic resistance. Biochimica et Biophysica Acta. 2009;1794:808–816.

Fenical W, Jensen PR. Developing a new resource for drug discovery: marine actinomycete bacteria. Nat Chem Biol. 2006;2:666–73.

Holguin G, Vazquez P, Bashan Y. The role of sediment microorganisms in the productivity, conservation, and rehabilitation of mangrove ecosystems: an overview. Biology and Fertility of Soils. 2001;33(4):265-78.

Isaka M, Suyarnsestakorn C, Tanticharoen M. Aigialomycins A–E, new resorcylic macrolides from the marine mangrove fungus Aigialus parvus. J Org Chem. 2002;67:1561–1566.

Kronemberger FA, Anna LM, Fernandes AC, Menezes RR, Borges CP, Freire DM. Oxygen-controlled biosurfactant production in a bench scale bioreactor. In Biotechnology for Fuels and Chemicals. Humana Press. 2007;401-413.

Poch GK, Gloer JB. Auranticins A and B: two depsidones from a mangrove isolate of the fungus Preussia aurantiaca. J Nat Prod. 1991;54:213–217

Jain R, Jain SK, Screening of In vitro Cytotoxic Activity of Some Medicinal Plants Used Traditionally to Treat Cancer in Chhattisgarh State, India, Asian Pacific Journal of Tropical Biomedicine. 2011;S147- S150

Bhanot A, Sharma R, Noolvi MN, Natural Sources as Potential Anticancer Agents: A Review International Journal of Phytomedicine. 2011;3:09- 26.

Gunduz M, Gunduz E: Preface. In: Breast Cancer – Carcinogenesis, Cell Growth and Signalling Pathways. Rijeka, InTech, p XI, 2011.

Done SJ: Preface. In Breast Cancer - Recent Advances in Biology, Imaging and Therapeutics. Rijeka, InTech, p IX; 2011.

Warcup JH. The Soil-Plate Method for Isolation of Fungi from Soil. Nature. 1950;166:117-118.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry. 1979;95(2):351-8.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J. Biochem. Biophys. 1984;21: 130–132.

Sinha KA. Colorimetric assay of catalase. Anal. Biochem. 1972;47:389–394.


Moron MS, De Pierre JM, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase in rat lung and liver. Biochim. Biophys Acta. 1979;582:67–78.


Stach JEM, Maldonado LA, Ward AC, Goodfellow M, Bull AT. New primers for the class Actinobacteria: Application to marine and terrestrial environments. Environ Microbiol. 2003;5(10):828-841.

GhanemNB, Sabry SA, El-Sherif ZM, GehanAAE.Isolation and enumeration ofmarine Actinomycetes from sea water and sediments in Alexandria. J Gen Appl Microbiol. 2000;46:05–111.

Williams ST, Davies FL. Use of antibiotics for selective isolation and enumeration of actinomycetes in soil. J Gen Microbiol. 1965;38:251–6.

Bredholt H, Fjaervik E, Johnsen G, Zotchev SB. Actinomycetes from sediments in the Trondheim Fjord, Norway: diversity and biological activity. Mar Drugs. 2008;6:12–24.

Terahara T, Kobayashi T, Imada C. An effective method based on wet-heat treatment for the selective isolation of Micromonospora from estuarine sediments? World J Microbiol Biotechnol .2013;29(9):1677–84.

Williams ST, Lanning S, Wellington EMH. Ecology of actinomycetes. In: Goodfellow M, Mordarski M, Williams ST, editors. The biology of actinomycetes. London: Academic Press. 1984;481–528.

Baskaran R, Vijayakumar R, Mohan PM. Enrichment method for the isolation of bioactive actinomycetes from Mangrove sediments of Andaman Islands, India. Malays J Microbiol. 2011;7:26–32.

Talbot GH, Bradley J, Edwards JE Jr, Gilbert D, Scheld M, Bartlett JG. Bad bugs need drugs: an update on the development pipeline from the antimicrobial availability task force of the infectious diseases society of America. Clin Infect Dis. 2006;42(5): 657-668

Bentley SD, Chater KF, Cerdeno-Tarraga AM, Challis GL, Thompson NR, James KD, Harris DE, Quail MA, Kieser H, Harper D. Complete genome sequence of the model actinomycetes Streptomyces coelicolor A3(2). Nature. 2002;417:141– 147.

Hong K, Gao AH, Xie QY, Gao H, Zhuang L, Lin HP, Yu HP, Li J, Yao XS, Goodfellow M, Ruan JS. Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China. Marine drugs. 2009;7(1): 24-44.

Jeffrey LSH, Sahilah AM, Son R, Tosiah S. Isolation and screening of actinomycetes from Malaysian soil for their enzymatic and antimicrobial activities. J Trop Agric Food Sci. 2007;35:159-164.

Das A, Mazumder Y, Dutta BK, Shome BR, Bujarbaruah KM, Sharma G. Clostridium perfringens type A beta-2 toxin in elephant (Elephas maximus indicus) and pygmy hog (Sus salvanius) with haemorrhagic enteritis in Assam, India. Afr J Microbiol Res. 2008;2(8):196-201.

Ghasemi, M.; Turnbull, T.; Sebastian, S.; Kempson, I. The mtt assay: Utility, limitations, pitfalls, and interpretation in bulk and single-cell analysis. Int. J. Mol. Sci. 2021;22:12827.

Bashan Y, Moreno M, Troyo E. Growth promotion of the seawater-irrigated oilseed halophyte Salicornia bigelovii inoculated with mangrove rhizosphere bacteria and halotolerant Azospirillum spp. Biology and Fertility of Soils. 2000;32(4):265-72.

Berdy J. Bioactive microbial metabolites. J Antibiot. 2005;58:1–26.

Simon HU, Haj-Yehia A, Levi-Schaffer F. Apop- tosis 2000;5: 415–418.

Shilpa PN, Sivaramakrishnan V, Niranjali Devaraj S. Asian Pac. J. Cancer. Prev. 2012;13:2753–2758.

Karthikeyan S, Hoti SL, Prasad NR. Biomed. Pharmacother. 2015;70:274–282.

Anderson AS, Wellington MHE. The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol. 2001;51:797-814.