Improved Production and Physicochemical Stability of a Bioemulsifier from a Marine Acinetobacter beijerinckii PHCS 7

PDF

Published: 2023-02-28

DOI: 10.56557/upjoz/2023/v44i23399

Page: 25-34


Devaraj Dharmadevi

Department of Microbiology, Faculty of Science, Annamalai University, Annamalai Nagar, Chidambaram - 608002, Tamil Nadu, India.

Baskaran Aravindan

Department of Microbiology, Faculty of Science, Annamalai University, Annamalai Nagar, Chidambaram - 608002, Tamil Nadu, India.

Punamalai Ganesh

Department of Microbiology, Faculty of Science, Annamalai University, Annamalai Nagar, Chidambaram - 608002, Tamil Nadu, India.

Kandasamy Sivasubramani *

Department of Microbiology, Faculty of Science, Annamalai University, Annamalai Nagar, Chidambaram - 608002, Tamil Nadu, India.

*Author to whom correspondence should be addressed.


Abstract

The commercial use of bioemulsifiers on various fields still faces the hindrance mainly because of low productivity. Hence, the current study inclines to improve the synthesis of a bioemulsifier from a marine Acinetobacter beijerinckii PHCS 7, which was previously isolated from a sediment sample polluted with petroleum hydrocarbons and this study also reports on the stability of the extracted emulsifier. During the optimization of biotic and abiotic factors, the use of 1% of trehalose, 0.5% yeast extract, 0.5% coconut oil, 200 rpm agitation, 30 ppt salinity and 2% of inoculum size evidenced improved production of bioemulsifier. Large-scale emulsifier synthesis was carried out based on the optimised conditions, followed by diethyl ether extraction and the stability of the extracted bioemulsifier was characterized. The findings demonstrated that the extracted bioemulsifier was stable under wide range of extreme situations, including those involving temperature, pH, and salinity. The improved synthesis and physicochemical stability of a bioemulsifier from a marine A. beijerinckii PHCS 7 reveal the wide spectrum of its uses in both bioindustrial and environmental domains.

Keywords: Acinetobacter beijerinckii, bioemusifier, emulsification, production, optimization, stability


How to Cite

Dharmadevi, D., Aravindan, B., Ganesh, P., & Sivasubramani, K. (2023). Improved Production and Physicochemical Stability of a Bioemulsifier from a Marine Acinetobacter beijerinckii PHCS 7. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(2), 25–34. https://doi.org/10.56557/upjoz/2023/v44i23399

Downloads

Download data is not yet available.

References

Huijer K. Trends in oil spills from tanker ships 1995-2004. ITOPF. 2005;30.

Ritchie W. A Consideration of the Environmental Component of the IMO Guidelines on Places of Refuge for Ships in Need of Assistance with Special Reference to Oil Pollution. In Places of Refuge for Ship. 2006; 75-91.

Keramea P, Spanoudaki K, Zodiatis G, Gikas G, Sylaios G. Oil spill modeling: A critical review on current trends, perspectives, and challenges. J. Mar. Sci. Eng. 2021;9(2): 181.

Karanth NGK, Deo PG, Veenanadig NK. Microbial production of biosurfactants and their importance. Curr. Sci. 1999;116-126.

Panjiar N., Sachan S.G. and Sachan A. Screening of bioemulsifier-producing micro-organisms isolated from oil-contaminated sites. Ann. Microbiol. 2015; 65(2):753-764.

Bicca FC, Fleck LC, Ayub MAZ. Production of biosurfactant by hydrocarbon degrading Rhodococcus ruber and Rhodococcus erythropolis. Rev. de Microbiol. 1999;30: 231-236.

Dastgheib SMM, Amoozegar MA, Elahi E, Asad S, Banat IM. Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery. Biotechnol. Lett. 2008;30(2):263-270.

Maneerat S. Production of biosurfactants using substrates from renewable-resources. Songklanakarin J. Sci. Technol. 2005;27(3):675-683.

Das P, Soumen M, Ramkrishna S. Improved bioavailability and biodegradation of a model polyaromatic hydrocarbon by a biosurfactant producing bacterium of marine origin. Chemosphere. 2008;72(9):1229-1234.

Nishanthi R, Sekar K, Palani P, Chellaram C, Prem Anand T, Venkat K. Screening of biosurfactants from hydrocarbon degrading bacteria. J. Ecobiotechnol. 2010;2(5).

Saimmai A, Onkamon R, Theerawat O, Vorasan S, Suppasil M. Isolation and functional characterization of a biosurfactant produced by a new and promising strain of Oleomonas sagaranensis AT18. World J. Microbiol. Biotechnol. 2012;28(10):2973-2986.

Makkar RS, Cameotra SS. Utilization of molasses for biosurfactant production by two Bacillus strains at thermophilic conditions. JAOCS. 1997;74(7):887-889.

Ron Z., Rosenberg E. Emulsifying activities of purified alasan proteins from Acinetobacter radioresistens KA53. AEM. 2001;67(3):1102-1106.

Dharmadevi D, Ganesh P, Sivasubramani K. Delving of a promising bioemulsifier producing bacterium from an oil contaminated coastal site and its enhanced production. Biosci. Biotechnol. Res. Asia. 2022;19(3):727-35.

Camacho-Chab JC, Guézennec J, Chan-Bacab MJ, Ríos-Leal E, Sinquin C, Muñiz-Salazar R and Ortega-Morales B.O. Emulsifying activity and stability of a non-toxic Bioemulsifier synthesized by Microbacterium sp. MC3B-10. Int. J. Mol. Sci. 2013;14(9):18959-18972.

Plaza Grazyna A, Ireneusz Zjawiony, Ibrahim Banat. Use of different methods for detection of thermophilic biosurfactant-producing bacteria from hydrocarbon-contaminated bioremediated soils. J. Pet. Sci. Eng. 2006;50(1):71-77.

Kuyukina MS, Irena B. Ivshina JC, Philp NC, Sandra AD, Marina IR. Recovery of Rhodococcus biosurfactants using methyl tertiary-butyl ether extraction. J Microbiol. Methods. 2001;46(2):149-156.

Ashtaputre AA, Shah AK. Emulsifying property of a viscous exopolysaccharide from Sphingomonas paucimobilis. World J Microbiol Biotechnol. 1995;11(2):219-222.

Desai, Jitendra D, Ibrahim M. Banat. Microbial production of surfactants and their commercial potential. Microbiol. Mol. Biol. Rev. 1997;61(1):47-64.

Mahdi El, Abdullah M, Hamidi Abdul Aziz, Salem S, Abu Amr, Nour Sh El-Gendy, Hussein N. Nassar. Isolation and characterization of Pseudomonas sp. NAF1 and its application in biodegradation of crude oil. Environ Earth Sci. 2016; 75(5):1-11.

Kazim AR, Hassan HM, Ahmed SA. Production, Optimization and Application of Bioemulsifier Extracted from Pseudomonas aeruginosa. World J. Exp. Biosci. 2017;5:9-13.

Lopes EM, Tereza Cristina Luque Castellane, Cristiane Moretto., EG de M Lemos., Souza JAM. Emulsification properties of bioemulsifiers produced by wild-type and mutant Bradyrhizobium elkanii strains. J. Bioremed. Biodeg. 2014; 5(6):245.

Ahmed EF, Shatha SH. Effect of media composition (carbon and nitrogen sources) on the production of bioemulsifier from Serratia marcescens S10. J. Biotechnol. Res. Center. 2012; 6(2):9-14.

Moussa TAA, Mohamed MS, Samak N. Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN. Braz. J. Chem. Eng. 2014;31:867-880.

Adetunji AI, Ademola OO. Production and characterization of bioemulsifiers from Acinetobacter strains isolated from lipid-rich wastewater. 3 Biotech. 2019;9(4): 1-11.

Ferreira INS, Dayana MR, Galba MC-Takaki, Rosileide F. da SA, Biosurfactant and bioemulsifier as promising molecules produced by Mucor hiemalis isolated from Caatinga soil. Electron. J. Biotechnol. 2020;47:51-58.

Markande AR, Acharya SR, Nerurkar AS. Physicochemical characterization of a thermostable glycoprotein bioemulsifier from Solibacillus silvestris AM1. Process Biochem. 2013;48(11):1800-1808.