Antibacterial Potential of Holothuria leucospilota, Brandt 1835 Extracts from Carmen Bay, Romblon, Philippines

Jeric B. Gonzalez *

School of Fisheries and Technology, Romblon State University-San Agustin Campus, Cabolutan, San Agustin, Romblon-5501, Philippines.

Grethel M. Mores

School of Fisheries and Technology, Romblon State University-San Agustin Campus, Cabolutan, San Agustin, Romblon-5501, Philippines.

*Author to whom correspondence should be addressed.


This study evaluated the antibacterial activity of extracts from the skin, Polian vesicle, Cuveirian tubules, and intestines of sea cucumber Holothuria leucospilota was evaluated against Staphylococcus aureus (Gram-positive bacteria) and Escherichia coli (Gram-negative bacteria). The disc diffusion method evaluated the antibacterial activity of sea cucumber H. leucospilota extracts. All treatments show inhibitory effects in both test microorganisms however cuvierian tubules have no effect on E. coli. Among the six treatments tested in S. aureus, the skin has the highest zone of inhibition with an average of 23.25mm followed by the intestine at 14.75mm, positive control at 10.25mm, and Polian vesicle at 9.00mm, respectively. The majority of the treatments were classified as bacteriostatic towards the test microorganisms.  Based on the findings of this study, the Gram-positive bacteria, S. aureus was more susceptible than the Gram-negative bacteria, E. coli. Therefore, it was concluded that sea cucumber extracts especially H. leucospilota collected in Carmen Bay, Romblon, Philippines, exhibited antibacterial potential towards test bacteria. The results of this study suggest that H. leucospilota is a potential source for discovering new antibiotics. Thus, this species has a potential for the pharmaceutical industry. It also suggests further investigations need to be carried out to determine its potential application in other aspects of medicine. In addition, elucidation of the compounds responsible for the bioactive including identification, sufficient isolation, and purifications, and also the analysis of the antibacterial mode of action should be done for natural drug development in the future.

Keywords: Antibacterial activity, aqueous extract, Holothuria leucospilota, S. aureus, E. coli

How to Cite

Gonzalez, J. B., & Mores, G. M. (2023). Antibacterial Potential of Holothuria leucospilota, Brandt 1835 Extracts from Carmen Bay, Romblon, Philippines. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(6), 12–22.


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Maloy KS, Sivakumar K, Kannan L. Marine realm: A Treasure House for Bioprospecting. Asian J Microbiol Biotechnol Environ Sci. 2007;9:191-196. Available:

Malve H. Exploring the ocean for new drug developments: Marine pharmacology. J Pharm Bioallied Sci. 2016;8(2):83-91. Available:

Shakouri A, Shoushizadeh M R, Nematpour F. Antimicrobial Activity of Sea Cucumber (Stichopus variegatus) Body Wall Extract in Chabahar Bay, Oman Sea. Jundishapur J Nat Pharm Prod. 2017;12(1):e32422


Lawrence AJ, Afifi R, Ahmed M, Khalifa S, Paget T. Bioactivity as an options value of sea cucumbers in the Egyptian Red Sea. Conserv Biol. 2010;24(1):217-225. Available:

Mehmet A, Huseyin S, Bekir T, Yilmaz, E, Sevim, K. Proximate composition and fatty acids profile of three different fresh and dried commercial sea cucumbers from Turkey. Int. J. Food Sci. Technol. 2011;46, 500-508.


Haug T, Kjuul AK, Styrvold OB, Sandsdalen E, Olsen ØM, Stensvåg K. Antibacterial activity in Strongylocentrotus droebachiensis (Echinoidea), Cucumaria frondosa (Holothuroidea), and Asterias rubens (Asteroidea). J Invertebr Pathol. 2002;81(2):94-102. Available:

Faukner J. Marine natural products. Nat. Prod. Rep. 2002;13:75-125. Available:

Sithranga Boopathy N, Kathiresan K. Anticancer drugs from marine flora: an overview. J Oncol. 2010; 21: 41-86.


Al-Haj N, Shamsudin M, Mohamad H, Vairappan C, Sekawi Z. Antibacterial activity of marine source extracts against multidrug resistance organism. Am. J. Pharm. Toxic. 2010;5(2): 95-102.


Haug T, Kjuul AK, Stensvåg K, Sandsdalen E, Styrvold OB. Antibacterial activity in four marine crustacean decapods. Fish Shellfish Immunol. 2002;12(5):371-385


Mokhlesi A, Saednia S, Gohari AR, Shahverdi AR, Nasrolahi A, Farahani F, Khoshnood R, Rshaghi N. Biological activities of the Sea cucumber Holothuria leucospilota. Asian J Anim Vet Adv. 2012; 7: 243-249.


Cayabo GD, Mabuhay-Omar JA. Antibacterial potential of crude extracts from sea cucumber Holothuria fuscoscinerea Jaeger, 1833. Cayabo & Mabuhay-Omar: Antibacterial potential of sea cucumber


Bonnet M, Lagier JC, Raoult D, Khelaifia S. Bacterial culture through selective and non-selective conditions: the evolution of culture media in clinical microbiology. New Microbes New Infect. 2019;34:100622.


Clinical and Laboratory Standards Institute (CLSI). Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts, Approved Guideline. CLSI document M44-A. CLSI, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898, USA, 2004. Available:

Bordbar S, Anwar F, Saari N. High-Value Components and Bioactives from Sea Cucumbers for Functional Foods—A Review. Mar. Drugs. 2011;9:1761-1805. Available:

Khotimchenko Y. Pharmacological Potential of Sea Cucumbers. Int J Mol Sci. 2018;19(5):1342.


Zhao F, Ma T, Zhang X, Zao Q, Zhu K, Cao J, Liu Z, Shen X, Li C. Holothuria leucospilota Polysaccharides Improve Immunity and the Gut Microbiota in Cyclophosphamide-Treated Immunosuppressed Mice [published online ahead of print, 2022 Nov 19]. Mol Nutr Food Res. 2022;e2200317. Available:

Lu S, Yuan Y, Chen F, Zheng Y, Li C, Cao J, Xia G, Lui Z, Shen X, He Y, Zhou D, Zhu K. Holothuria Leucospilota polysaccharides alleviate hyperlipidemia via alteration of lipid metabolism and inflammation-related gene expression. J Food Biochem. 2022; 46(12):e14392. Available:

Zhang X, Zhu K, Zeng S, Zheng Y, Cao J, Li C. Microbiome-Metabolomics Analysis Reveals the Mechanism of Holothuria leucospilota Polysaccharides (HLP) in Ulcerative Colitis [published online ahead of print, 2023 Mar 22]. Mol Nutr Food Res. 2023;e2200633. Available:

Yuan Y, Liu Q, Zhao F, Cao J, Shen X, Li C. Holothuria Leucospilota Polysaccharides Ameliorate Hyperlipidemia in High-Fat Diet-Induced Rats via Short-Chain Fatty Acids Production and Lipid Metabolism Regulation. Int J Mol Sci. 2019;20(19): 4738. Available:

Aminin DL, Menchinskaya ES, Pisliagin EA, Silchenko AS, Avilov SA, Kalinin VI. Anticancer Activity of Sea Cucumber Triterpene Glycosides. Mar. Drugs 2015; 13(3),1202-1223. Available:

Ibrahim HA. Antibacterial carotenoids of three Holothuria species in Hurghada, Egypt. Egypt. J. Aquat. Res. 2012;38:185-194. Available:

Schillaci D, Cusimano MG, Cunsolo V, et al. Immune mediators of sea-cucumber Holothuria tubulosa (Echinodermata) as source of novel antimicrobial and anti-staphylococcal biofilm agents. AMB Express. 2013;3(1):35. Available:

Abubakar L, Mwangi C, Uku J, Ndirangu S. Antimicrobial activity of various extracts of the sea urchin Tripnuestes gratilla (Echinoidea). Afri. J. Pharma. and Therapeutics. 2012;1(1): 19-23.


Adibpour N, Nasr F, Nematpour F, Shakouri A, Ameri A. Antibacterial and Antifungal Activity of Holothuria leucospilota Isolated From Persian Gulf and Oman Sea. Jundishapur J Microbiol. 2014;7(1):e8708 Available:

Blunt, JW, Copp BR, Munro M.H.G, Northcote PT, Prinsep, MR. Marine natural products. Nat. Prod. Rep. 2007; 24(1):31-86 Available:

Pankey GA, Sabath LD. Clinical relevance of bacteriostatic versus bactericidal mechanisms of action in the treatment of Gram-positive bacterial infections. Clin Infect Dis. 2004;38(6):864-870.


Piri-Gharaghie T, Ghajari G, Hassanpoor M, Jegargoshe-Shirin N, Soosanirad M, Khayati S, Farhadi-BireganiA, Mirzaei A. . Investigation of antibacterial and anticancer effects of novel niosomal formulated Persian Gulf Sea cucumber extracts. Heliyon. 2023;9(3):e14149. Available:

Ebrahimi H, Vazirizadeh A, Nabipour I, Najafi A, Tajbakhsh S, Nafisi Bahabadi M. In vitro study of antibacterial activities of ethanol, methanol and acetone extracts from sea cucumber Holothuria parva. Iran. J. Fish. Sci. 2018; 17(3):542-551. Available:

Sikkema J, de Bont JA, Poolman B. Interactions of cyclic hydrocarbons with biological membranes. J Biol Chem. 1994;269(11):8022-8028. Available:

Shan B, Cai YZ, Brooks JD, Corke H. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. Int J Food Microbiol. 2007; 117(1):112-119 Available:

Kalemba D, Kunicka A. Antibacterial and antifungal properties of essential oils. Curr Med Chem. 2003;10(10): 813-829. Available:

Sasidharan S, Darah I, Noordin MK. In vitro antimicrobial activity against Pseudomonas aeruginosa and acute oral toxicity of marine algae Gracilaria changii. N Biotechnol. 2010;27(4): 390-396. Available: