Anti-inflammatory Activity of Asparagus officinalis Extract on Liver Inflammation Carrageenan-Induced in Swiss Albino Mice
Doaa M. Elnagar *
Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Egypt and Zoology Department, College of Science, King Saud University, Saudi Arabia.
*Author to whom correspondence should be addressed.
Abstract
The present work was designed to evaluate the anti-inflammatory impact of Asparagus officinalis (AO) extract on liver inflammation CGN-induced.
Materials and Methods: Forty male Swiss albino mice were divided randomly into four groups, 10/each. 1st group kept as untreated control, 2nd group treated daily with oral dose of (500 mg/kg) AO extract for one week, 3rd group treated with ip single dose of 2%W/V carrageenan (CGN) and left for one week, 4th group treated with carrageenan as group 3 and AO extract as group 2.
Results: CGN injection resulted in significant increase of WBCs and platelets, elevation of liver enzymes and proinflammatory cytokines (TNF-α and IL6). Morphological feature of tails showed severe necrosis, histopathological examination of tail tissue displayed heavy incidence of inflammatory cells in dermis layer and liver revealed severe inflammation, steatosis and ballooning. Meanwhile, post-treatment with AO extract after CGN showed significant decrease of WBCs, platelets and physiological parameters. Histopathological analysis revealed less incidence of inflammation in tail and liver tissue besides to improvement of tissues and pathological score.
Conclusion: It was concluded that AO extract possesses intense anti-inflammatory effect on liver inflamed by CGN.
Keywords: Asparagus officinalis, extract, carrageenan, inflammation, histopathology