Isolation and Characterization of Nitrogen Fixing Bacteria from Rhizospher of Chickpea and Peanodules, Hossana, Ethiopia

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Published: 2023-07-29

DOI: 10.56557/upjoz/2023/v44i153574

Page: 97-107


Denebo Sebaro Wanore *

Department of Biology, College of Natural and Computational Sciences, Wahemo University, P.O. Box-667, Hossana, Ethiopia.

Mekonin Antuye Bachore

Department of Biotechnology, College of Natural and Computational Sciences, Wachemo University, P.O. Box-667, Hossana, Ethiopia.

Essayas Kebede Eromo

Department of Biology, College of Natural and Computational Sciences, Wahemo University, P.O. Box-667, Hossana, Ethiopia.

Rehima Kebato Nure

Department of Biotechnology, College of Natural and Computational Sciences, Wachemo University, P.O. Box-667, Hossana, Ethiopia.

Gezahegn Batebo Bidiko

Department of Chemical Engineering, College of Engineering and Technology, Wachemo University, P.O. Box-667, Hossana, Ethiopia.

Shambel Selman Abdo

Department of Statistics, College of Natural and Computational Sciences, Wachemo University, P.O. Box-667, Hossana, Ethiopia.

*Author to whom correspondence should be addressed.


Abstract

One of the ways to increase the competitive survivability of rhizobial bio fertilizers and achieve better plant growth under such conditions is by modifying the rhizospheric environment or community by addition of non rhizobial nodule-associated bacteria (NAB) that cause better nodulation and plant growth when co-inoculated with rhizobia. To achieve this objective experimental design was carried out. Rhizobium is the root of legumes host nitrogen fixing bacteria which can invade root and get sugars from the plant. A study was performed to investigate the most commonly associated nodule-associated bacteria and the rhizospheric microorganisms associated with the legume plant. Present study on Isolation and characterization of Nitrogen Fixing Bacteria from agricultural rhizosphere was carried out with the aim of Isolation and characterization of Nitrogen Fixing Bacteria. The occurrence of Nitrogen Fixing Bacteria from root nodule of pea, white chickpea and red chickpea were investigated. Nitrogen fixing bacteria was isolated from root nodule of pea, white chickpea and red chickpea. The isolates were grown on yeast extract – mannitol agar (YEMA), and the morphologic characterization was done by Gram stain. A total of 21 isolates were isolated on Yeast Extract Mannitol (YEMA) agar medium. The isolates were circular, and irregular in form with smooth surface; entire, colony margins; with light pink coloured. The identification of isolated pure cultures through colony morphology analysis, cellular morphology and biochemical properties are including gram staining (negative), catalase test (negative), Methyl red test (negative), voges proskaeur test (positive), citrate utilization test (positive), starch hydrolysis test (positive) and mannitol salt agar (positive). Among the 21 bacterial isolates isolated, 18 were shown the characteristic of rhizobia. The effect of these 18 isolates those shows the characteristics of rhizobia were performed and all of them are significant, while compared with controls. These isolates will be useful to produce efficient bio fertilizers for Agriculture.

Keywords: Chickpea, isolation, nitrogen fixation, nodule associated bacteria, pea, rhizobia


How to Cite

Wanore , D. S., Bachore , M. A., Eromo , E. K., Nure, R. K., Bidiko , G. B., & Abdo , S. S. (2023). Isolation and Characterization of Nitrogen Fixing Bacteria from Rhizospher of Chickpea and Peanodules, Hossana, Ethiopia. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(15), 97–107. https://doi.org/10.56557/upjoz/2023/v44i153574

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References

Johnston AW, Todd JD, Curson AR, Lei S, Nikolaidou-Katsaridou N, Gelfand MS, Rodionov DA. Living without Fur: the subtlety and complexity of iron-responsive gene regulation in the symbiotic bacterium Rhizobium and other α-proteobacteria. Biometals. 2007;20(3-4):501-511.

Nghia NH, Gyurjan. Problems and perspectives in establishment of nitrogen- fixing symbioses and endosymbioses. Endocyt. C. Res. 1987;4:131-141.

Abdalla MH, Issa AA, Ohyama T. Impact of harsh environmental conditions on nodule formation and di nitrogen fixation of legumes. Advances in biology and ecology of nitrogen fixation. ISBN. 2014; 978-953.

Howieson J, Committee GRP. Technical issues relating to agricultural microbial genetic resources (AMiGRs), including their characteristics, utilization, preservation and distribution: Draft information paper; 2007.

Matiru VN, Dakora FD. Potential use of rhizobial bacteria as promoters of plant growth for increased yield in landraces of African cereal crops. African Journal of Biotechnology. 2004;3:1-7.

Fisher RF, Long SR. Rhizobium-plant signal exchange. Nature (London). 1992; 357:655-660.

Suliasih Sri Widawati. Isolation and identification of phosphate solubilizing and nitrogen fixing bacteria from soil inwamena biological garden, Jayawijaya, Papua Biodivercitas ISSN: 1412-033x. Halaman. 2005;6(5):175-177.

Hardason G, WL. Broughton (Ed.). Maximizing the use of Biological Nitrogen Fixation in Agriculture. Kuwer Academic Publishers and Food and Agriculture Organisation of the United Nations International Atomic Energy Agency; 2003.

Beveridge TJ. Use of the gram stain in microbiology, Biotechnic and Hist-chemistry. 2001;76(3):111-8.

Phyllis A, Martin W, Haransky EB, Travers RS, Reichelderfer CI. Rapid Biochemical Testing of large number of Bacillus thuringiensis Isolates Using Agar dots. Biotechniques. 1985;3(5):386-392.

Barea MJ, Pozo R Azc, Azc C. on-Aguilar. Microbial co-operation in the rhizosphere. Journal of Experimental Botany. 2005;56 (417):1761–1778,.

Sturz BR, Christie BG, Matheson, Nowak J. Biodiversity of endophytic bacteria which colonize red clover nodules, roots, stems and foliage and their influence on host growth. Biology and Fertility of Soils. 1997;25(1):13–19.

Pervin S, Jannat B, Sanjee S, Farzana T. Characterization of rhizobia from root nodule and rhizosphere of Lablab purpureus and Vigna sinensis in Bangladesh. Turkish J. Agric. -Food Sci. Technol. 2017;5(1):14-17.

Aggarwal A, Kadian N, Karishma Neetu TA, Gupta KK. Arbuscular mycorrhizal symbiosis and alleviation of salinity stress. J.Appl. Nat. Sci. 2012;4(1): 144-155.

Barron JE, Pasini RJ, Davis DW, Stuthrnan DD, Graha PH. Response to selection for seed yield and nitrogen fixation in common bean (Phasealus vulgaris L.) Filed Crops Research 2000;62:119-128.

Dilworth EK, James JI, Sprent WE. Newton, Nitrogen-fixing leguminous symbioses, Verlag Berlin Heidelberg. 2006;58-59.

Graham P, Rondi P. Common bean (Phaseolus vulgaris).Field Crop Research. 1997;53:131-146.

Gyongyver M, Tamás E, Eva L, Eva G, Istvan M, Beata A, Szabolcs L. Microbial products as natural alternative to fertilisers: Isolation and characterisation of nitrogen fixing bacteria .U.P.B. Sci. Bull., Series B. 2010;72(3). ISSN 1454-2331.

Heszky L, Fésüs L, Hornok, Mezőgazdasági biotechnológia, Edit. Agroinform, Budapest. 2005;103.

Hungria M, Vargas MAT. Environmental factors affecting N2 fixation in grain legumes in the tropics, with an emphasis on Brazil. Field Crops Res. 2000;65:151-164.

Mohammadi K, Sohrabi Y, Heidari G, Khalesro S, Majidi M. Effective factors on biological nitrogen fixation. Afr. J. Agric. Res. 2012;7(12):1782-1788.

Mukerji C, Manoharachary J. Singh, Microbial Activity in the Rhizosphere, Springer.

Rajendran F, Singh A, Desai J, Archana G. Enhanced growth and nodulation of pigeon pea by co-inoculation of bacillus strains along with Rhizobium sp. Bioreource Technology. 2008;99:4544–4550.