Chemical Composition, Antioxidant and Antimicrobial Activities of the Aqueous Extract of Syzygium aromaticum (Clove) from Indonesia


Published: 2023-10-06

DOI: 10.56557/upjoz/2023/v44i213686

Page: 178-191

Lynda Sabrina Ounaceur *

Biochemistry and Environmental Toxicology Laboratory, Faculty of Sciences, Badji Mokhtar University of Annaba, Algeria.

Mabrouka Bouacha

Biochemistry and Environmental Toxicology Laboratory, Faculty of Sciences, Badji Mokhtar University of Annaba, Algeria.

Oumaima Taguida

Biochemistry and Environmental Toxicology Laboratory, Faculty of Sciences, Badji Mokhtar University of Annaba, Algeria.

Nesrine Djaber

Biochemistry and Environmental Toxicology Laboratory, Faculty of Sciences, Badji Mokhtar University of Annaba, Algeria.

Mahfoud Messarah

Biochemistry and Environmental Toxicology Laboratory, Faculty of Sciences, Badji Mokhtar University of Annaba, Algeria.

Amel Boumendjel

Biochemistry and Environmental Toxicology Laboratory, Faculty of Sciences, Badji Mokhtar University of Annaba, Algeria.

*Author to whom correspondence should be addressed.


The clove (Syzygium aromaticum) is an aromatic herb that originates mainly from Central Asia and Africa. This herb has been used in many ways, especially in the medical field. Given its many therapeutic benefits, it would be interesting to identify the different phytochemicals of this plant. The primary objective of this work is to measure the various phenolic compounds in the aqueous extract of the plant and to identify them, using the HPLC technique. The study of the antioxidant activity in vitro of this extract as well as its antimicrobial activity have been carefully carried out. According to the results of the phytochemical analysis, this plant contained, more or less, significant amounts of phenolic compounds such as total phenols (176.02 mg), flavonoids (23.5 mg) and ortho-diphenols (740,14 mg). Moreover, some phenolic compounds, in particular flavonols, were present in smaller amounts, (2.09 x 10-3 mg). The presence of certain compounds was then confirmed by the HPLC technique. The results of the study of the antioxidant activity of clove extract (DPPH, ABTS and β-carotene test), showed values that ​​were high. As for the antibacterial activity tests, they showed remarkably high inhibition zones and sensitivity (diameter 18mm and 15mm diameter) for the Gram negative bacteria : Pseudomonas aeruginosa and Escherichia Coli. As regards for the gram-positive strain which is Staphylococcus aureus, it had a high sensitivity and an inhibition diameter of (19 mm).

Keywords: Syzygium aromaticum, phytochemical screening, HPLC, antioxidant and antimicrobial activities

How to Cite

Ounaceur , L. S., Bouacha , M., Taguida , O., Djaber , N., Messarah , M., & Boumendjel , A. (2023). Chemical Composition, Antioxidant and Antimicrobial Activities of the Aqueous Extract of Syzygium aromaticum (Clove) from Indonesia. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(21), 178–191.


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