DISTRIBUTIONAL PATTERN OF DIFFERENT CELL TYPES WITH SPECIAL EMPHASIS ON SEASONAL CHANGES OF GONADOTROPHS IN THE HYPOPHYSIS OF INDIAN RIVER SHAD Gudusia chapra (HAMILTON, 1822) IN RELATION TO OVARIAN MATURITY

Main Article Content

SHRABANI BARUN

Abstract

Reproduction is a natural phenomenon and it performs under the influence of various external factors which mediate their effect through the hypothalamo-hypophyseal-gonadal subsystem operating as an organic axis bridging the ecological influences and gonadal maturation. Information is collected through the hypothalamus and is passed on to the hypophysis. Hypothalamus releases GnRH which exerts its effect on hypophysis/pituitary to release gonadotrophin hormone from gonadotrophs of Pituitary. Gonadotrophic hormone helps in gonadal maturation and sex steroid production which plays a very important role in oogenesis, final maturation of oocytes. The Pituitary gland of G. chapra is cranio-leptobasic type. It is generally composed of neurohypophysis and adenohypophysis.  The adenohypophysis is divided of three parts viz., rostral pars distalis (RPD), middle proximal pars distalis (PPD) and massive pars intermedia (PI). The major part of the RPD occupied by the acidophilic prolactin cells (PRL) stained with acid fuchsin. Fewer adrenocorticotropic cells (ACTH) were scattered among prolactin cells. The basophilic gonadotrophs (GTH) and thyrotrophs (TSH) reacted positively to aniline blue and periodic acid Schiff’s (PAS) were distributed in the anterior and middle part of PPD. The somatotrophs (STH) were the only acidophils that identified. Melanocyte stimulating hormone (MSH) stained with aniline blue and melanocyte concentrating hormone (MCH) stained with acid fuchsin were identified in PI. During growth and maturation phase both GTH and TSH cells were distinguished by intense staining and dense homogenous granules with maximum cellular diameter. During post-spawning phase both cells showed low staining intensity. The seasonal changes in the ovary of G. chapra have been described on the basis of variation in GSI value and frequency percentage of the different female germ cells. The gradual change in the ovarian cycle has been correlated with changes of GTH and TSH cells in pituitary of Gudusia chapra.

Keywords:
Gudusia chapra, gonadotrophs, GSI, hypophysis, ovarian cycle

Article Details

How to Cite
BARUN, S. (2019). DISTRIBUTIONAL PATTERN OF DIFFERENT CELL TYPES WITH SPECIAL EMPHASIS ON SEASONAL CHANGES OF GONADOTROPHS IN THE HYPOPHYSIS OF INDIAN RIVER SHAD Gudusia chapra (HAMILTON, 1822) IN RELATION TO OVARIAN MATURITY. UTTAR PRADESH JOURNAL OF ZOOLOGY, 39(4), 151-165. Retrieved from http://mbimph.com/index.php/UPJOZ/article/view/1413
Section
Original Research Article

References

Hoar WS. The gonads and reproduction. In: The Physiology of fishes. Ed. M. E. Brown, Academic Press, New York. 1957;1:287-317.

Lal KK, Pandey AK. Hypothalamo-neurosecretory system of the female sea bass, Lates calcarifer (Bloch), with special reference to gonadal maturation. Indian J. Fish. 1998;45: 51-60.

Wen J, Xie J, Gui J. DNA clone and characterization of a novel SNX gene differentially expressed in previtellogenic oocytes of gibel carp. Comp. Biochem. Physiol. 2003;136B:451-461.

Ursani TJ, Shaikh SA, Soomro MH, Malik S. Histological changes in pituitary gonadotrophic hormone cells in relation to seasonal changes in ovaries of the fish Oreochromis mossambicus. Sindh Univ. Res. Journal (Sci. Ser). 2012; 44(1):07-14.

Mallory FB. The aniline blue collagen stain. Stain Technology. 1936;11:101.

Gomori G. Observations with differential stains on human islets of Langer hans. Amer. J. Pate. 1941;17(3):395.

Slidders W. The OFG and Br AB-OFG methods for staining the adenohypophysis. J. Pathol. 1961;82(2):532-534.

McManus JFA. Histological and histochemical uses of periodic acid. Stain Technology. 1948;23(3):99-108.

Agulleiro B, Garcia-Hernandez MP, Garcia Ayala A. Teleost adenohypophysis: Morpho-functional and development aspects. In: Reinecke MP, Zaccone G, Kapoor BG. (eds). Fish Endocrinology. Vol.1. Science Publishers, Enfield. 2006;289-440.

Narayan A, George KC, Diwan AD. Morpho-histology of the pituitary gland of the estuarine teleost fish, Valamugil cunnesius. Proc. Indian Acad. Sci. (Anim. Sci.,). 1984;93(6):565-571.

El-Sakhawy MA, El-Saba AA, Abd Rabou MI, El-Shammaa MA, Hussein SH. Seasonal histology of the testes of Nile Tilapia (Oreochromis niloticus). J. Vet. Anat. 2011a; 4(2):61-75.

Chatterjee N, Chakrabarti P. Distributional pattern of different cells with special emphasis on the seasonal variations of gonadotrophs in the pituitary gland of Mystus vittatus (Bloch) in relation to testicular activities. Int. J. Fish. Aqua. Stud. 2014;2(1):79-87.

Bose S, Chakrabarti P. Distribution of different cells and seasonal variations of gonadotropin cells in the pituitary gland of Liza parsia (Ham.) in relation to maturation of ovary. J. Zool. Stud. 2015;1:33-49.

Jafri SIH, Ensor DM. Cell types in the pituitary of the roach, Rutilus rutilus (L.). J. Anat. 1980;130(4):667-672.

Sathyanesan AG. Correlative cyclical changes in the pituitary and gonads of Mystus seenghala (Sykes) and Barbus stigma (cuv. and Val.). J. Zoological Soc., India. 1960;12(2):175- 190.

Assem SS. Histochemical studies, cell type, distribution and seasonal variation of gonadotropin cells in pituitary gland of female Dicentrarchus labrax in relation to maturation of gonads. Egypt. J. Aqua. Res. 2004;30:374-389.

Khan H. Histophysiology of the hypophysis of Colisa fasciata (Bloch and Schneider). J. Inland Fish. Soc., India. 1974;6:18-32.

Jose TM, Sathyanesan AG. Pituitary cytology of the Indian carp Labeo rohita (Ham.). Anat. Anz. 1977;142(4):410-423.

Mandal S, Sinha GM. Adenohypophysial cell types in an Indian freshwater major carp, Catla catla (Ham.). Gegenbaurs Morphol. Jaharb. 1985;131:81-92.

Sathish SV, Sathishagouda S, Kulkarni RS, Patil SJ. Histochemical and electron micro-scopic studies on the pituitary gonadotropic cells in relation to different age group of fish Notopterus notopterus. Int. J. Current Res. Life Sciences. 2018;7(2):1163-1172.

Nobrega RH, Oliveira de Jesus LW, Honji RM, Borella MI. Characterization of gonadotropic cells during continuous and seasonal spermatogenesis of two freshwater fish species: A histochemical and immunohisto chemical study. Fish physiol. Biochem. 2016;6:267-275.

El-Gohary NMA. The effect of water quality of the reproductive biology of the Nile tilapia, Oreochromis niloticus in Lake Manzalah, Ph. D. thesis. Faculty of Science, Ain Shams University; 2001.

El-Sakhawy MA, El-Shammaa MA, Abd Rabou MI, El- Saba AA, Hussein SH. Seasonal histology and histochemistry of the adenohypo-physis of Nile Tilapia (Oreochromis niloticus). J. Vet. Anat. 2011b;4(2):39-60.

Chakrabarti P, Ray S. Season induced changes in the liver cells and ovarian tissues during growth, maturation, spawning and post-spawning phases in spiny eel, Mastacembelus armatus. Proc. Zool. Soc. 2016;201(6):81-88.

Mayer I, Shackley SE, Witthame PR. Aspects of the reproductive biology of the bass, Dicentrachus labrax. II. Fecundity and pattern of oocyte development. J. Fish Biol. 1990;36: 141-148.

Chakrabarti P, Gupta AK. A histological study of the testes during maturation and spawning phases in the exotic fish, Puntius javanicus (Blkr.). Advances in Biosciences. 1994;13:11-22.

Chellappa S, Camara MR, Verani JR. Ovarian development in the Amazonian red discus, Symphysodon discus. Braz. J. Biol. 2005;65(4): 609-616.

Abu-Hakima R. Aspects of the reproductive biology of the grouper, Epinephelus tauvina (Forskal) in Kuwati waters. J. Fish Biol. 1987;213-222.

Lee MF, Huang JD, Chang CF. Development of ovarian tissue and female germ cells in the protandrous black porgy, Acanthopagrus schlegeli (Perciformes, sparidae), Zool. Stud. 2008;47(3):302-316.

Lone KP, Hussain A. Seasonal and age related variations in the ovaries of Labeo rohita (Ham.). A detailed gross and histological study of gametogenesis, maturation and fecundity. Pak. J. Zool. 2009;41(3):217-239.

Htun-Han M. The reproductive biology of the dab, Limanda limanda (Ham) in the North Sea: Seasonal changes in the testes. J. Fish Biol. 1978;13:361-368.

Noorits T. Seasonal changes in the ovaries of common bream, Abramis brama L. in Lake Vortsjurv. Proc. Acad. Sci., Estonian SSR. Biol. 1984;33:1-6.

Janssen R. Follicles in competition. Nature, London. 1994;367:10.

Vohra A. Studies on breeding and colouration behaviour of some freshwater ornamental fishes of Jammu. Ph. D. thesis, University of Jammu; 2011.

Gadekar GP. Studies on the seasonal histomor-phological changes in the ovary of Indian major carp, Labeo rohita (Ham.). The Bioscan. 2014;9(3):1037-1042.

Sharma J, Sharma S, Kant KR. Ovarian development and reproductive cycle of Garra gotyla gotyla. The Bioscan. 2015;10(1):9-15.

Agarwal SC. A hand book of fish farming; 2008.

Hina. Reproductive biology of biodiversity of ornamental fish from Jammu water bodies. Ph. D. thesis, University of Jammu, Jammu; 2010.

Subha BR, Meheta SN. Ovarian histomorpho-logy and gonadal cycle of freshwater Garfish Xenentodon cancila. Bibechcra. 2012;8:96-104.

Chakrabarti P, Barun S. Histological organization and surface ultrastructure of ovaries of Gudusia chapra during different phases of reproduction. Iran. J. Ichthyol. 2017;4(1):41-53.

Santos RN, Andrade CC, Santos AFG, Santos LN, Araujo FG. Histological analysis of ovarian development of the characiform Oligosarcus hepsetus (Cuvier) in a Brazilian reservoir. Braz. J. Biol. 2005;65(1): 169-177.

Kundu S. Hypothalamo-hypophyseal system of Puntius sarana sarana (Ham.) in relation to gonadal maturation and spawning. Ph. D. thesis. 2018;1-232.

Barr WA. The endocrine control on the sexual cycle in the plaice, Pleuronectes platessa (L.). II. The endocrine control on oogenesis. Gen. Comp. Endocrinol. 1963a;3:197-204.

Rai BP. On the histophysiology of the pituitary gland in conjunction with the testicular cycle in the mahseer, Tor (Barbus) tor (Ham). Cell Tissue Organs. 1966b;65(1-3):416-434.

Nazaki M. Hypothalamic-pituitary-Gonadal endocrine system in the hagfish. Frontiers in Endocrinology. 2013;4:200.

Khanna SS, Pant MC. Cyclic changes in the pituitary gland of Glyptothorax pectinopterus (Mc Clelland) in correlation with its reproductive cycle. Cell Tissue Organs. 1969;72(1):148-157.