IDENTIFICATION AND OCCURRENCE OF Vibrio parahaemolyticus IN SHRIMP PONDS OF SELECTED PONDS IN PONNERI, TIRUVALLUR DISTRICT
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Published
Oct 3, 2019
Page:
41-48
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R. SANGEETHA
P.G. & Research Department of Advanced Zoology and Biotechnology, Loyola College, University of Madras, Chennai, Tamil Nadu, India.
M. CHRISTINA PAULINE
P.G. & Research Department of Advanced Zoology and Biotechnology, Loyola College, University of Madras, Chennai, Tamil Nadu, India.
PREMRAJ LOGANATHAN
P.G. & Research Department of Advanced Zoology and Biotechnology, Loyola College, University of Madras, Chennai, Tamil Nadu, India.
J. M. V. KALAIARASI
P.G. & Research Department of Advanced Zoology and Biotechnology, Loyola College, University of Madras, Chennai, Tamil Nadu, India.
Abstract
The present study aimed to monitor the occurrence of Vibrio parahaemolyticus, an opportunistic prawn pathogen, in two white-leg shrimp Litopenaeus vannamei culture ponds (from stocking to harvesting period), each located in the villages Pullikulam and Kumarasirulapakkam of Thiruvallur District. They were selectively isolated, counted using serial dilution and confirmed by 16s rDNA sequencing. Our study showed that V. parahaemolyticus occupied one tenth of the total microbial load in the ponds. The count ranged from 1000 to 1600 CFU/ml in water at the time of stocking and varied with time period. The sediment always harbour more bacteria than water and the number would reach up to 35×104 CFU/g in the harvesting period. Both the ponds showed a steep increase on the 30th day of stocking and then there is a gradual decrease or increase in the water parameter. The occurrence of the bacteria was found to depend on the water and soil quality parameters that vary with timelines. The study showed that the bacterial count increased with the culture periods in both the ponds. This would serve to be an indirect evaluation for the health assessment of the shrimps and raises the need for continuous validation on their loads.
Keywords:
Vibrio parahaemolyticus, Litopenaeus vannamei, microbial load, Vibrio content, water quality parameters, soil quality parameters.
Article Details
How to Cite
SANGEETHA, R., PAULINE, M. C., LOGANATHAN, P., & KALAIARASI, J. M. V. (2019). IDENTIFICATION AND OCCURRENCE OF Vibrio parahaemolyticus IN SHRIMP PONDS OF SELECTED PONDS IN PONNERI, TIRUVALLUR DISTRICT. UTTAR PRADESH JOURNAL OF ZOOLOGY, 40(2), 41-48. Retrieved from https://mbimph.com/index.php/UPJOZ/article/view/1424
Section
Short Research Article
References
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Available:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603525/
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Available:https://www.ncbi.nlm.nih.gov/pubmed/25548045
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Chun-Hung Liu, Winton Cheng, Jung-Ping Hsu, Jiann-Chu Chen. Vibrio alginolyticus infection in the white shrimp Litopenaeus vannamei confirmed by polymerase chain reaction and 16S rDNA sequencing. Diseases of Aquatic Organisms. 2004;61:169–174.
Available:https://www.ncbi.nlm.nih.gov/pubmed/15584425
Available:http://blog.aqua.deals/current-status-and-outlook-of-indian-shrimp-aquaculture/
Luke AR, Allen Davis D, Patrick Saoud I, Boyd CA, Harvey JP, Boyd CE. Shrimp culture in inland low salinity waters. Rev Aquacult. 2010;2:191–208.
Available:https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1753-5131.2010.01036.x
Laxmappa B. Shrimp farming in India. The Aquaculturists. The International Aquafeed: Perendale Publishers Ltd; 2017.
Available:http://theaquaculturists.blogspot.com/2017/10/20102017-shrimp-farming-in-india.html
Ravichandran P, Jayanthi M. Training manual on shrimp farming. Central Institute for Brackish Water Aquaculture. 2006;Special Publication 30.
Osunla CA, Okoh AI. Vibrio pathogens: A public health concern in rural water resources in Sub-Saharan Africa. Int J Environ Res Public Health. 2017;14(10):1188.
Available:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664689/
Prathibha Bharathi Chittem, Sumanth Kumar Kunda. Study on the quality of water and soil from L. vannamei shrimp farming in coastal districts of Andhra Pradesh, India. International Research Journal of Environmental Sciences. 2017;6(8):1-6.
Available:http://www.isca.in/IJENS/Archive/v6/i8/1.ISCA-IRJEvS-2015-055.pdf
APHA. Standard methods for the examination of water and waste water. American Public Health Association, Edition; 2012.
Available:http://yabesh.ir/wp-content/uploads/2018/02/Standard-Methods-23rd-Perv.pdf
Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology. 1991;173(2):697–703.
Available:https://www.ncbi.nlm.nih.gov/pubmed/1987160
Zar JH. Biostatistical analysis. Prentice-Hall Inc., N.J. 1974;620.
Available:http://garfield.library.upenn.edu/classics1989/A1989R928600001.pdf
Angela L, et al. Impact of aquaculture practices on intestinal bacterial profiles of pacific whiteleg shrimp Litopenaeus vannamei. Microorganisms. 2019;7:493.
Available:https://www.mdpi.com/2076-2607/7/4/93
Ganesh A, Sunita Das, Chandrasekar Arun, Balamurugan. Monitoring of total heterotrophic bacteria and Vibrio spp. in an aquaculture pond. Current Research Journal of Biological Sciences. 2009;2(1):48-52.
Available:https://pdfs.semanticscholar.org/abec/e0443caa0510714f0bb17eff0e8c0
1efabc9.pdf
Lekshmy, Nansimole, Mini, Athira, Tresa. Occurrence of Vibrio cholerae in shrimp culture environments of Kerala, India. Indian J. Sci. Res. 2014;5(2):151-160.
Available:https://pdfs.semanticscholar.org/3573/69de28773c7032f50ed2376f6dcb
8e040f5c.pdf
Fernanda CG, et al. Microbiome of pacific whiteleg shrimp reveals differential bacterial community composition between wild, aquaculture and AHPND/EMS outbreak conditions. Scientific Reports. 2017;7(1):117-83.
Available:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603525/
Soto-Rodriguez, Sonia A, et al. Field and experimental evidence of Vibrio parahaemolyticus as the causative agent of acute hepatopancreatic necrosis disease of cultured shrimp (Litopenaeus vannamei) in Northwestern Mexico. Applied and Environmental Microbiology. 2015;81(5):1689-99.
Available:https://www.ncbi.nlm.nih.gov/pubmed/25548045
Pattukumar, Maloy Kumar Sahu, Murugan, Vijayabaskara Sethubathi, Sivakumar, Arul. Population of Vibrio parahaemolyticus (Pathogen) and bacillus (Beneficial Bacteria) in Penaeus Monodon (Fabricus, 1798) culture. On Line Journal of Biological Sciences. 2010;10(4):142-150.
Available:https://pdfs.semanticscholar.org/c9f7/73502a700ec715284085753dba
1519f768fc.pdf
Chun-Hung Liu, Winton Cheng, Jung-Ping Hsu, Jiann-Chu Chen. Vibrio alginolyticus infection in the white shrimp Litopenaeus vannamei confirmed by polymerase chain reaction and 16S rDNA sequencing. Diseases of Aquatic Organisms. 2004;61:169–174.
Available:https://www.ncbi.nlm.nih.gov/pubmed/15584425