KNOCK DOWN EFFECT OF Syzygium aromaticum AND Cinnamonam cassia PLANT VOLATILE AGAINST THE Aedes aegypti L. (DIPTERA: CULICIDAE)

Main Article Content

K. ELUMALAI
J. SASIKALA

Abstract

In the present study, knock down activity of Syzygium. aromaticum and Cinnamomum cassia were tested against the freshly emerged adults of Ae. aegypti in a condutive laboratory. The plant volatile oils were individually tested with different concentrations of 50, 100, 150 and 200 mg/mL concentrations concentrations as stated in the experiments larval mortality against the freshly moulted fourth in star larvae of Ae. Aegypt with the selected concentration of the plant volatile oil. After 24, 48, 72 hrs of exposure the mosquitoes dead and counted the percent mortality was calculated by using standard formula. In the present study, the knockdown activity of C. cassia indicated that the significant mortalities were observed at 200mg/mL concentration. In 50 mL S. aromaticum induced, 26.2, 34.2 and 38.4%, 100 mg/mL concentration 2.8, 46.8 and 52.8% in the 150 mg/mL concentration 62.2, 72.2 and 76.4% and in 200 mg/mL concentration of the essential oil, S. aromaticum induced significant knockdown effect of 82.2, 84.6 and 90.2% at 24, 48 and 72 hrs exposure period respectively on Ae. aegypti .In the same way C. cassia was tested with different concentrations larval mortality against the on Ae. aegypti 50, 100, 150 and 200 mg/mL. In 50 mg/mL concentration of the essential oil, C. cassia induced 24.8, 38.6 and 40.2%, 100 mg/mL concentration 36.2, 45.2 and 52.4%, in the same way the 150 mg/mL 64.4, 74.6 and 78.2%, in the 200 mg/mL concentration of the essential oil C. cassia induced significant knockdown effect of 80.4, 86.8 and 92.2% at 24, 48 and 72 hrs exposure period. It is inferred that the plant oils persuaded remarkable mortality on the test mosquitoes. Thus, the phytochemicals are eco-friendly, safer to non-target organisms.

Keywords:
Plant volatile oil Syzygium aromaticum, Cinnamonam cassia, knockdown activity.

Article Details

How to Cite
ELUMALAI, K., & SASIKALA, J. (2020). KNOCK DOWN EFFECT OF Syzygium aromaticum AND Cinnamonam cassia PLANT VOLATILE AGAINST THE Aedes aegypti L. (DIPTERA: CULICIDAE). UTTAR PRADESH JOURNAL OF ZOOLOGY, 41(8), 113-121. Retrieved from https://mbimph.com/index.php/UPJOZ/article/view/1573
Section
Original Research Article

References

Bouguerra Fouzia Tine Djebbar, Noureddine Soltani. Algerian Thymus vulgaris essential oil: Chemical composition and larvicidal activity against the mosquito Culex pipiens. International Journal of Mosquito Research. 2017;4(1):37.

Roselayne F. Furtado, Maria G. A. de Lima, Manoel Andrade Neto, José N.S. Bea, Maria G. de V. Silva. Larvicidal activity of essential oils against Aedes aegypti L. (Diptera: Culicidae) Neotrop. Entomol. Londrina. 2005;34(5).

Larvicidal activity of essential oils from brazilian plants against Aedes aegypti Mem Inst Oswaldo Cruz, Rio de Janeiro. 2004; 99(5):541-544.

Scott A. Ritchie, Gregor J. Devine. Confusion knock-down and kill of Aedes aegypti using metofluthrin in domestic settings: A powerful tool to prevent dengue transmission? Parasites & Vectors. 2013;6:262.

Govindarajan M. Larvicidal and repellent properties of some essential oils against Culex tritaeniorhynchus Giles and Anopheles subpictus Grassi (Diptera: Culicidae). Asian Pacific Journal of Tropical Medicine. 2011; 4(2):106-111.

Govindarajan M, Angelina GP. Larvicidal efficacy of Ficus benghalensis L. plant leaf extracts against Culex quinquefasciatus Say Aedes aegypti. 2010;107-111.

Govindarajan M. Larvicidal and repellent activities of Sida acuta Burm. F. (Family: Malvaceae) against three important vector mosquitoes. Asian Pacific Journal of Tropical Medicine. 2010;3(9):691-695.

Ishwarya R, Vaseeharan B, Kalyani S, Banumathi B, Govindarajan M. Facile green synthesis of zinc oxide nanoparticles using Ulva lactuca seaweed extract and evaluation of their photocatalytic, antibiofilm and insecticidal activity. Journal of Photochemistry and Photobiology B: Biology. 2018;178:249-258.

Mathivanan T, Govindarajan M, Elumalai K, Krishnappa K, Ananthan A. Mosquito larvicidal and phytochemical properties of Ervatamia coronaria Stapf. (Family: Apocynaceae). J Vector Borne Dis. 2010;47(3): 178-180.

Govindarajan M, Sivakumar R, Rajeswari M, Yogalakshmi K. Chemical composition and larvicidal activity of essential oil from Mentha spicata (Linn.) against three mosquito species. Parasitology Research. 2012;110(5):2023-2032.

Govindarajan M, Mathivanan T, Elumalai K, Krishnappa K, Anandan A. Mosquito larvicidal, ovicidal, and repellent properties of botanical extracts against Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus (Diptera: Culicidae). Parasitology Research. 2011;109(2):353-367.

Sayono S, Hidayati APN, Fahri S, et al. Distribution of voltage-gated sodium channel (NAV) alleles among the Aedes aegypti populations in central Java province and its association with resistance to pyrethroid insecticides. PLoS ONE. 2016;11(3):e0150577.

Murray NEA, Quam MB, Wilder-Smith A. Epidemiology of dengue: Past, present and future prospects. Journal of Clinical Epidemiology. 2013;5(1):299–309.

Carlson KM, Heilmayr R, Gibbs HK, et al. Effect of oil palm sustainability certification on deforestation and fire in Indonesia. Proceedings of the National Acadamy of Sciences of the United States of America. 2018;115(1):121–126.

Kweka EJ, Kimaro EE, Munga S. Effect of deforestation and land use changes on mosquito productivity and development in western Kenya highlands: Implication for malaria risk. Frontiers in Public Health. 2016;4:238.

Fonseca-Gonz´alez I, Qui˜nones ML, Lenhart A, Brogdon WG. Insecticide resistance status of Aedes aegypti (L.) from Colombia. Pest Management Science. 2011;67(4):430–437.

Hamid H, Ninditya VI, Prastowo J, Haryanto A, Taubert A, Hermosilla C. Hindawi current status of Aedes aegypti insecticide resistance BioMed Research International. 2019;19(3):1-15.

WHO. Global Strategy for Dengue Prevention and Control, 2012-2020, WHO Press, Geneva, Switzerland; 2012.

Govindarajan M. Bioefficacy of Cassia fistula Linn. (Leguminosae) leaf extract against chikungunya vector, Aedes aegypti (Diptera: Culicidae) Eur Rev Med Pharmacol Sci. 2009;13(2):99-103.

Du Y, Nomura Y, Satar G, et al. Molecular evidence for dual pyrethroid-receptor sites on a mosquito sodium channel. Proceedings of the National Acadamy of Sciences of the United States of America. 2013;110(29):11785–11790.

Govindarajan M, Karuppannan P. Mosquito larvicidal and ovicidal properties of Eclipta alba (L.) Hassk (Asteraceae) against chikungunya vector, Aedes aegypti (Linn.) (Diptera: Culicidae). Asian Pacific Journal of Tropical Medicine. 2011;4(1):24-23.

Seokyoung Kang, Dongyoung Shin, Mi Young Noh, Jill S. Peters, Chelsea T. Smartt, Yeon Soo Han, Young S. Hong. Optimization of double-stranded RNAi intra thoracic injection method in Aedes aegypti. Entomological Research. 2018;48:269–278.

Sheila Barbara G. Lopez, Victor Guimarães-Ribeiro, João Victor G. Rodriguez, Fernando A. P. S. Dorand, Tiago S . Salles, Thayane E. Sá Guimares, Evelyn S. L. Alvarenga, Ana Claudia A. Melo, Rodrigo V. Almeida, Monica F. Moreira. RNAi-based bioinsecticide for Aedes mosquito control Zika virus infection and dengue and chikungunya fevers are emerging viral diseases scientific report. 2019; 9:4038.

Ana Paula de Araújo, Marcelo Henrique Santos Paiva, Amanda Maria Cabral, Antônio Emanuel Holanda Dias Cavalcanti, Luiz Fernando Freitas Pessoa, Diego Felipe Araujo Diniz, Elisama Helvecio, Ellyda Vanessa Gomes da Silva, Norma Machado da Silva, Daniela Bandeira Anastácio, Claudenice Pontes, Vania Nunes, Maria de Fátima Marinho de Souza, Fernando Jorge Rodrigues Magalhães, Screening Aedes aegypti (Diptera: Culicidae) populations from Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron and Cypermethrin and Characterization of Potential Res. 2019;19(3): 16.

Govindarajan M, Sivakumar R, Rajeswary M, Yogalakshmi K. Chemical composition and larvicidal activity of essential oil from Ocimum basilicum (L.) against Culex tritaeniorhynchus, Aedes albopictus and Anopheles subpictus (Diptera). Experimental Parasitology. 2013; 134(1):7-11.

Saavedra-Rodriguez K, Urdaneta-Marquez L, Rajatileka S, et al. A mutation in the voltage-gated sodium channel gene associated with pyrethroid resistance in Latin American Aedes aegypti. Insect Molecular Biology. 2007;16(6): 785–798.

Kawada H, Higa Y, Komagata O, et al. Widespread distribution of a newly found point mutation in voltage-gated sodium channel in pyrethroid-resistant Aedes aegypti populations in Vietnam. PLOS Neglected Tropical Diseases. 2009;3(10):e527.

Srisawat R, Komalamisra N, Apiwathnasorn C, et al. Fieldcollected permethrin-resistant Aedes aegypti from central Thailand contain point mutations in the domain IIS6 of the sodium channel gene (kdr). Southeast Asian Journal of Tropical Medicine and Public Health. 2012;43(6):1380–1386.

Plernsub S, Saingamsook J, Yanola J, et al. Additive effect of knockdown resistance mutations, S989P, V1016G and F1534C, in a heterozygous genotype conferring pyrethroid resistance in Aedes aegypti in Thailand. Parasites & Vectors. 2016;9(1):417.

Alvarez LC, Ponce G, Saavedra-Rodriguez K, Lopez B, Flores AE. Frequency of V1016I and F1534C mutations in the voltage-gated sodium channel gene in Aedes aegypti in Venezuela. Pest Management Science. 2015;71(6):863–869.

Ishak IH, Jaal Z, Ranson H, Wondji CS. Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in the dengue vectors Aedes aegypti and Aedes albopictus from Malaysia. Parasites & Vectors. 2015; 8(1):181.

Garc´ıa GP, Flores AE, Fern´andez-Salas I, et al. Recent rapid rise of a permethrin knock down resistance allele in Aedes aegypti in M´exico. PLOS Neglected Tropical Diseases. 2009;3(10):e531.

Li CX, Kaufman PE, Xue RD, et al. Relationship between insecticide resistance and kdr mutations in the dengue vector Aedes aegypti in Southern China. Parasites & Vectors. 2015;8:325.

Hirata K, Komagata O, Itokawa K, Yamamoto A, Tomita T, Kasai S. A single crossing-over event in voltage-sensitive Na+ channel genes may cause critical failure of dengue mosquito control by insecticides. PLOS Neglected Tropical Diseases. 2014;8(8).

WHO. Test procedures for insecticide resistance monitoring in malaria vector mosquitoes, World Health Organization, Geneva; 2013.

Jayakumar M, Arivoli S, Raveen R, Samuel T. Proceedings of symposium on recent trends in combating mosquitoes. Journal of Entomology and Zoology Studies. 2016;4(5):449-456.

Hamid PH, Prastowo J, Widyasari A, Taubert A, Hermosilla C. Knockdown resistance (kdr) of the voltage-gated BioMed Research International 7 sodium channel gene of Aedes aegypti population in Denpasar, Bali, Indonesia. Parasites & Vectors. 2017;10(1).

Hamid PH, Prastowo J, Ghiffari A, Taubert A, Hermosilla C. Aedes aegypti resistance development to commonly used insecticides in Jakarta, Indonesia. PLoS ONE. 2017; 12(12).

Hemingway J, Hawkes NJ, McCarroll L, Ranson H. The molecular basis of insecticide resistance in mosquitoes. Insect Biochemistry and Molecular Biology. 2004;34(7):653–665.

Brengues C, Hawkes NJ, Chandre F, et al. Pyrethroid and DDT cross-resistance in Aedes aegypti is correlated with novel mutations in the voltage-gated sodium channel gene. Medical and Veterinary Entomology. 2003; 17(1):87–94.

Wuliandari JR, Lee SF, White VL, Tantowijoyo W, Hoffmann AA, Endersby-Harshman NM. Association between three mutations, F1565C, V1023G and S996P, in the voltage-sensitive sodium channel gene and knockdown resistancein Aedes aegypti from Yogyakarta, Indonesia. Insects. 2015;6(3):658–685.

Davies TGE, O’Reilly AO, Field LM, Wallace BA, Williamson MS. Knockdown resistance to DDT and pyrethroids: From target-site mutations to molecular modeling. Pest Management Science. 2008;64(11):1126– 1130.

Khan HAA, Akram W, Shehzad K, Shaalan EA. First report of field evolved resistance to agrochemicals in dengue mosquito, Aedes albopictus (Diptera: Culicidae), from Pakistan. Parasites & Vectors. 2011;4(1):146.

Kuan MM, Chang FY. Airport sentinel surveillance and entry quarantine for dengue infections following a fever screening program in Taiwan. BMC Infectious Diseases. 2012; 12(182).

Kotsakiozi P, Gloria-Soria A, Caccone A, et al. Tracking the return of Aedes aegypti to Brazil, the major vector of the dengue, chikungunya and Zika viruses. PLOS Neglected Tropical Diseases. 2017;11(7):e0005653.

Govindarajan M. Chemical composition and larvicidal activity of leaf essential oil from Clausena anisata (Willd.) Hook. f. ex Benth (Rutaceae) against three mosquito species. Asian Pacific Journal of Tropical Medicine. 2010;3(11):874-877.

Pushpanathan T, Jebanesan A, Govindarajan M. The essential oil of Zingiber officinalis Linn (Zingiberaceae) as a mosquito larvicidal and repellent agent against the filarial vector Culex quinquefasciatus Say (Diptera) Parasitology Research. 2008;102(6):1289-1291.

Govindarajan M, Jebanesan A, Pushpanathan T, Samidurai K. Studies on effect of Acalypha indica L.(Euphorbiaceae) leaf extracts on the malarial vector, Anopheles stephensi Liston (Diptera: Culicidae). Parasitology Research. 2008;103(3):691.

Kolanjinathan K, Ganesh P, Govindarajan M. Antibacterial activity of ethanol extracts of seaweeds against fish bacterial pathogens. Eur Rev Med Pharmacol Sci. 2009;13(3):173-177.

Govindarajan M, Jebanesan A, Reetha D, Amsath R, Pushpanathan T. Antibacterial activity of Acalypha indica L Eur Rev Med Pharmacol Sci. 2008;12(5):299-302.

Benelli G, Mehlhorn H. Declining malaria, rising of dengue and Zika virus: Insights for mosquito vector control. Parasitology Research. 2016;115(5):1747–1754.

WHO, Dengue, Countries or Areas at Risk, WHO Press, Geneva, Switzerland; 2012.

Powell JR, Tabachnick WJ. History of domestication and spread of Aedes aegypti–a review,” Mem´orias do Instituto Oswaldo Cruz. 2013;108:11–17.

Afrane YA, Zhou G, Lawson BW, Githeko AK, Yan G. Effects of microclimatic changes caused by deforestation on the survivorship and reproductive fitness of Anopheles gambiae in western Kenya highlands. The American Journal of Tropical Medicine and Hygiene. 2006;74(5):772–778.

Tsuda Y, Suwonkerd W, Chawprom S, Prajakwong S, Takagi M. Different spatial distribution of Aedes aegypti and Aedes albopictus along anurban-rural gradient and the relating environmental factors examined in three villages in northern Thailand. Journal of the American Mosquito Control Association. 2006;22(2):222–228.

Morrison AC, Zielinski-Gutierrez E, Scott TW, Rosenberg R. Defining challenges and proposing solutions for control of the virus vector Aedes aegypti. PLoS Medicine. 2008; 5(3):e68.

Lima EP, Paiva MHS, de Ara´ujo AP, et al. Insecticide resistance in Aedes aegypti populations from Cear´a, Brazil. Parasites & Vectors. 2011;4(1):5.

Harris AF, Rajatileka S, Ranson H. Pyrethroid resistance in Aedes aegypti from Grand Cayman. The American Journal of Tropical Medicine and Hygiene. 2010;83(2):277–284.

Yanola J, Somboon P, Walton C, Nachaiwieng W, Somwang P, Prapanthadara LA. High-throughput assays for detection of the F1534C mutation in the voltage-gated sodium channel gene in permethrin-resistant Aedes aegypti and the distribution of this mutation throughout Thailand. Tropical Medicine & International Health. 2011;16(4):501–509.

Kushwah RBS, Dykes CL, Kapoor N, Adak T, Singh OP. Pyrethroid-resistance and presence of two knockdown resistance(kdr)mutations, F1534Cand a Novel Mutation T1520I, in Indian Aedes aegypti,” PLOS Neglected Tropical Diseases. 2015;9(1):e3332.

Zhou X, Yang C, Liu N, Li M, Tong Y, Zeng X, Qiu X. Knockdown resistance (kdr) mutations within seventeen field populations of Aedes albopictus from Beijing China: First report of a novel V1016G mutation and evolutionary origins of kdr haplotypes. Parasites & Vectors. 2019;12(1):180.

Abozeid S, Elsayed AK, Schaffner F, Samy AM. Unexpected outbreaks of arbovirus infections: Lessons learned from the Pacific and tropical America. Lancet Infect Dis. 2018; 18:e355-e361.

Zhou XJ, Yang C, Liu N. Detection of the invasive mosquito species Aedes albopictus in southern England. Lancet Infect Dis. 2017;17: 140.

WHO. Factsheet on the World Malaria Report 2013; 2014.

[Retrieved Aug 20, 2014]

World Health Organization. Manual on practical entomology in malaria. Part II.WHO Division of Malaria and Other Parasitic Diseases, Geneva; 1975.

Govindarajan M, Mathivanan T, Elumalai K, Krishnappa K, Anandan A. Ovicidal and repellent activities of botanical extracts against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi (Diptera: Culicidae). Asian Pacific Journal of Tropical Biomedicine. 2011;1(1):43-48.

Stenhouse SA, Plernsub S, Yanola J, et al. Detection of the V1016G mutation in the voltage-gated sodium channel gene of Aedes aegypti (Diptera: Culicidae) by allele-specific PCR assay, and its distribution and effect on deltamethrin resistance in Thailand. Parasites & Vectors. 2013;6(1): 253.

Govindarajan M, Rajeswary M, Hoti SL, Bhattacharyya A, Benelli G. Eugenol, α-pinene and β-caryophyllene from Plectranthus barbatus essential oil as eco-friendly larvicides against malaria, dengue and Japanese encephalitis mosquito Vectors Parasitology Research. 2016;115(2):807-815.