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The aim of this work was to assess the influence of different osmolyte concentrations on the viability and dormancy of free living Acrobelus nematode. A high-temperature (50°C) assay was done to check dormancy and viability. An extreme decrease in viability was noted when juveniles were exposed to high-temperature assay whereas non-desiccated juveniles died within short exposure to high temperature. Using the high-temperature assay, the mortality of desiccated nematodes was slow representing an improved capability to tolerate high-temperature conditions. At high osmolyte concentration (60%) maximum dormancy was observed. Variable recovery periods were noted when nematodes were exposed to two different temperatures. Low glycerol concentration at low temperature results in less recovery time.
Crowe JH, Crowe LM, Chapman D. Preservation of membranes in anhydrobiotic organisms: The role of trehalose. Science. 1984;223:701–703.
Crowe JH, Crowe LM. Membrane integrity in anhydrobiotic organisms: Towards a mechanism for stabilizing dry cells. In Water and Life (G. N. Somerso, C. B. Osmond, and C. L. Bolis, Eds.). Springer-Verlag, Berlin. 1992;87–103.
Panek AD. Trehalose metabolism—New horizons technological applications. Brazil. J. Med. Biol. Res. 1995;28:169–181.
Itamar G, Liora S. Osmotic survival of the entomopathogenic nematode Steinernema carpocapsae. Department of Nematology, ARO, Volcani Center, Bet Dagan 50-250, Israel; 1999.
Songbi C, Itamar G. Effect of rapid and gradual increase of osmotic stress on survival of entomopathogenic nematodes. Phytoparasitica. 2004;32:486-497.
Chen S, Yang H, Jiang S. Studies on the biochemical characters of Steinernema carpocapsae BJ in anhydrobiosis. Acta Parasitol Med. Entomol. Sin. 2000;7:30-34.
Chen S, Yang H, Jiang S. Morphology and oxygen consumption of entomopathogenic nematode Steinernema carpocapsae BJ in anhydrobiosis. Acta Entomol. Sin. 2001;44:62-66.
Finnegan MM, Downes JD, O'Regan M, Griffin CT. Effect of salt and temperature stresses on the survival and infectivity of Heterorhabditis spp. infective juveniles. Nematology. 1999;1:69-78.
Stephenson W. The effect of variations in osmotic pressure upon a free-living soil nematode. PH.D., Department of Zoology, King's College, University of Durham, Newcastle-on-Tyne; 1942.
Forster SJ. Osmotic stress tolerance and osmoregulation of intertidal and subtidal nematodes. Department of Biological Sciences, Queen Mary and Westfield College, University of London, Mile End Road, London, E1 4NS; 1997.
David A. Osmoregulation in the Antarctic nematode Panagrolaimus davidi. Wharton Department of Zoology, University of Otago, P.O.Box 56, Dunedin; 2010.
Schopfer WH. Recherches physiochimiquessur le milieu intérieur de quelques parasites. Première partie. Rev. Suisse Zol. 1932;39:59–194.
Panikkar NK, Sproston NG. Osmotic relations of some metazoan parasites. Parasitology. 1941;33:214-23.
Wright DJ. Respiratory, physiology, nitrogen excretion and osmotic and ionic regulation. In: Perry, R.N. and Wright, D.J. [Eds.] The Physiology and Biochemistry of Free-Living and Plant-Parasitic Nematodes. CABI Publishing, London, UK. 1998;103-132.
Wharton DA. The environmental physiology of Antarctic terrestrial nematodes: A review. J. Comp. Physiol. B Biochem. Syst. Environ. Physiol. 2003;173:621-628.
Tim RW. Antarctic soil and freshwater nematodes from the McMurdo Sound region. Proceedings of the Helminthological Society of Washington. 1971;38:42–52.
Porazinska DL, Wall DH, Virginia RA. Invertebrates in ornithogenic soils on Ross Island, Antarctica. Polar Biol. 2002;25:569-574.
Fuse M, Davey KG. Osmoregulation in the parasitic nematode Pseudoterranova decipiens. Department of Biology, York University, North York, Ontario, Canada M3J 1P3 and R. I. Sommerville. Department of Zoology, University of Adelaide, Adelaide, South Australia; 1992.
Keith P. Choe. Physiological and molecular mechanisms of salt and water homeostasis in the nematode Caenorhabditis elegans. Am J Physiol Regul Integr Comp Physiol. 2013;305: 175–186.
Seo Y, Kim YH. Control of Meloidogyne incognita using mixtures of organic acids. Plant Pathol J. 2014;30:450–455.
Jang JY, Choi YH, Shin TS, Kim TH. Biological control of Meloidogyne incognita by Aspergillus niger F22 producing oxalic acid. PLoS ONE. 2016;11: e0156230.
Dawson D, Liu C, Xuehong. Osmoregulation: Some principles of water and solute transport. In: David H. Evans (Ed). Osmotic and Ionic Regulation: Cells and Animals. CRS Press, Florida, USA; 2009.
Demeure Y, Freckman DW. Recent advances in the study of anhydrobiotic nematodes. In Plant Parasitic Nematodes (B. M. Zuckerman, and R. A. Rohde, Eds.), Academic Press, New York. 1981;3:205–226.
Glazer I, Orion D. Studies on anhydrobiosis of Pratylenchus thornei. J. Nematol. 1983;15: 333–338.
Sikkink KL, Reynolds RM, Ituarte CM, Cresko WA, Phillips PC. Rapid evolution of phenotypic plasticity and shifting thresholds of genetic assimilation in the nematode Caenorhabditis remanei. G3 (Bethesda). 2014;4:1103–1112.
John M, Olaf S, Lieven W, Nicole V, Maurice M, Ehlers RU. Heat tolerance among different strains of the entomopathogenic nematode Heterorhabditis bacteriophora. Biocontrol; 2010.
Crowe JH, Madin KAC. Anhydrobiosis in nematodes: Evaporative water and survival. J. Exp. Zool. 1975;193:323–334.