SIGNIFICANT ALTERATIONS IN THE BIOCHEMICAL PARAMETERS OF GILLS OF THREE EDIBLE SHELLFISHES DUE TO HEAVY METAL POLLUTION LOAD OF ASHTAMUDI LAKE, INDIA

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V. LEKSHMI PRIYA
E. SHERLY WILLIAMS

Abstract

Biochemical alterations in the gills of three edible shellfishes Scylla serrata (mud crab), Penaeus monodon (tiger prawn) and Paphia malabarica (yellow foot clam) inhabiting the polluted and non-polluted regions of Ashtamudi Lake were assessed using standard methods. The results of the Fisher’s LSD (Least significant difference) Post hoc multiple comparison in the gill samples of S. serrata and P. monodon, reveals that site 1 and 2 (Polluted sites) significantly differ from site 3 (reference/ non polluted site) with respect to carbohydrate, fat, ash and moisture, except protein which significantly differ among all the sites. Considering the gills of Paphia malabarica, site 1 and 2 significantly differ from site 3 in the case of carbohydrate, fat, protein, and moisture, except ash, which was significantly, differ among the three sites. When compared with the samples of the non polluted / reference site significant reductions in the proximate compositions has been monitored in the tissues of the selected species collected from the polluted sites. The results clearly indicates the fact that shellfishes undergo physiological stress, when exposed to heavy metal contamination, and their biochemical synthesis is severely altered by pollutants.

Keywords:
Heavy metals, pollution, Scylla serrata, Penaeus monodon, Paphia malabarica, gills, biochemical constituents

Article Details

How to Cite
PRIYA, V. L., & WILLIAMS, E. S. (2020). SIGNIFICANT ALTERATIONS IN THE BIOCHEMICAL PARAMETERS OF GILLS OF THREE EDIBLE SHELLFISHES DUE TO HEAVY METAL POLLUTION LOAD OF ASHTAMUDI LAKE, INDIA. UTTAR PRADESH JOURNAL OF ZOOLOGY, 41(11), 75-80. Retrieved from https://mbimph.com/index.php/UPJOZ/article/view/1638
Section
Original Research Article

References

Authman MMN, Zaki MS, Khallaf EA, Abbas HH. Use of fish as bio-indicator of the effects of heavy metals pollution. J. Aquat. Res. Dev. 2015;6:4-13.

Azmat H, Javed M, Jabeen G. Acute toxicity of aluminium to the fish (Catla catla, Labeo rohita and Cirrhina mrigala). Pakistan Vet. J. 2012;31:85-87.

Nurnadia AA, Azrina A, Amin I. Proximate compositionand energetic value of selected marine fish and shellfish from the West coast of Peninsular Malaysia. Int. Food Res. J. 2011; 18:137-148.

Tsape K, Sinanoglu VJ, Miniadis-Meimaroglou S. Comparative analysis of the fatty acid and sterol profiles of widely consumed Mediterranean crustacean species. Food Chem. 2011; 122:292–299.

Dinakaran GK, Soundarapandian P, Tiwary AK. Nutritionalmstatus of edible palaemonid prawn Macrobrachium scabriculum (Heller, 1862). Eur. J. Appl. Sci. 2010;2 :30-36.

Kavitha, C., Malarvizh, A., Senthil, K.S. and Ramesh, M. 2010. Toxicological effects of arsenate exposure on haematological, biochemical and liver transaminase activity in an Indian major carp, Catla catla. Food Chem. Toxicol. 48(28): 48-54.

Swapna, P. and Ravinder, R. T. 2015. Proximate, amino acid, fatty acid and mineral analysis of bivalve Parreysia cylindrica from Waddepally and Kaleshwaram Lake. World Journal of Pharmacy and Pharmaceutical Sciences, 4(4): 1388-1401.

Arafa, M. M., Al-Afifi, SH. H. and Ali, A. T. 2015. Investigating the Oxidative Stress of Heavy Metal’s Pollution in Clarias Gariepinus .Egypt. J. Chem. Environ. Health., 1(1): 231-243.

Rajalakshmi, P. 2015. Effect of lead nitrate on biochemical composition of blood and acid phosphatase activity in Cyprinus carpio (Linnaeus). Current Biotica., 9 (3):256-262.

Celine, H.M.S, Bhuvaneswari D.and Anandan R. 2015. Biochemical and histopathological studies on lead nitrate induced toxicity in fresh water fish grass carp (Ctenopharyngodon Idella). European Journal of Experimental Biology.,5(11):24-30.

Razeena,K.L. 2014. Ecobiological aspects of an estuarine fish Lisa Parsia (Doctoral dissertation), University of Kerala.

Girish Kumar, B. 2016. Toxicity induced changes of Cu and Pb on the humoral and cellular factors on Anabas testudineus (Bloch, 1792) (Doctoral dissertation),Cochin University of Science and Technology.

Lekshmi, P.V and Sherly. W.E. 2018. An assessment of heavy metal pollution load of ashtamudi lake with respect to the mangrove crab, scylla serrata. Eco chronicle.4 (13): 203-208.

Dubois, M., Gilles, K.A. Hamilton, J.K. Rebers P.A. and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem., 28: 350-356.

Lowry, O.H., Rosebrough, N.J. Farr A.L. and Randall, R.J. 1951. Protein measurement with Folin-Phenol reagents. J. Biol. Chem., 193: 265-275.

Folch, J., Lees M. and Slone- Satanley G.H., 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226: 497-509.

Narasimhan,N.,Maharajothi,P.,Ravichelvan,R.,Sukumaran, M., Ravichandran, R. and Rengarajan R. 2013. Biochemical studies on protein content in different tissues of selected shrimps penaeus monodon and penaeus vannamei . International journal of pure and applied zoology., 1(1): 92 – 94.

Appukuttan, K.K. 1993. Studies on the ecobiology and fishery of Paphia malabarica (Chemnitz), Veneridae, Bivalvia from Ashtamudi estuary, SouthWest coast of India. PhD thesis, University of Kerala,185p.

Wilson, C., Santamaría, A.T., Tabares, Y.,Velasco, S. M. 2019. Science of The Total Environment Biochemical and histological alterations in Aequidens metae(Pisces,Cichlidae)and Astyanaxgr.bimaculatus (Pisces,Characidae) asindicators of river pollution., 692: 1234-1241.

Puvaneswari, S. Jiyavudeen M. and Venkatesh. P. 2017. Serum biochemical alterations in fish Mugil cephalus collected from Uppanar river, Cuddalore, Tamilnadu, India. Life Science Archives., 3 (2): 1029 – 1034.

Samson, E. A., 2013 .Evaluation of the Haematology and Biochemistry of Clarias gariepinus as Biomakers of Environmental Pollution in Tiga dam, Nigeria Brazilian archives of biology and technology., 56(3): 371-376.

Ramesh, A., Sukumaran, M. and Rengarajan, R. 2018.Toxic effect of copper sulphate on protein alterations in esturine fish Mugil cephalus, International Journal of Multidisciplinary., 3(11): 2455-3085.

Lasiene K., Straukas D., Vitkus A. and Juodziukyniene N. 2016. The influence of copper sulphate pentahydrate (CuSO4•5H2O) on the embryo development in the guppies (Poecilia reticulata). Ital. J. Ani. Sci., 15: 529 - 535.

Azza,H. M, Sherin, K.S, Gamalat, Y. S and Hoda, H. A.A. 2014. Toxic effects of heavy metals pollution on biochemical activities of the adult brine shrimp, Artemia salina. Senra Academic Publishers, British Columbia., 8(3): 3019-3028.

Trivedi,M.H., Sangai N.P. & Renuka A. 2012. Assessment of toxicity of copper sulphate pentahydrate on oxidative stress ındicators on liver of gold fish (Carassius auratus). Bull. Environ. Pharmacol. Life Sci., 1: 52 – 57.

Sandhya, M. S., Mayur, S. 2018. Effect Of Heavy Metals Hgcl2 And Cdcl2 On Glycogen Activity Of Bivalve Lamellidens Marginali, Journal Of Pharmacy. 8(8): 28-35.

Kawade S. J and Khillare Y. K. 2012. Toxicity of zinc on the biochemical contents of certain tissues of freshwater fish, Channa gachua (ham.). International Journal of Applied Biology and Pharmaceutical Technology., 3(3): 243.

Sonia, A and Ali, M.Y. 2017. Chromium toxicity in fish: A review article. Journal of Entomology and Zoology Studies., 5(3): 1483-1488.

Pundir,G and Pundir, H. 2015. Impact of Heavy metal, Arsenic trioxide on Biochemical profile of teleost, Clarias batrachus (Linn.), Int. J. of Life Sciences., 3(2): 141-146.

Dahunsi, S.O., Oranusi, S.U and Ishola, R.O. 2011. Biochemical profile of clarias gariepinus exposed to sub-lethal concentrations of chemical additives effluent. International Journal of Research in Environmental Science and technology .,1 (4): 52-58.

Patil. H.Z.,Mahale P.N.,and Kulkarni. B. 2011.Alteration in biochemical content in foot of Fresh water Snail,Indoplanorbis exustus exposed to Heavy metals .J.Expt Sci., 2: 12-14.

Demir Y., Oruc E. and Topal A. 2016. Carbonic anhydrase activity responses and histopathological changes in gill and liver tissues after acute exposure to chromium in brown trout juveniles. Hacettepe J. Biol. Che., 44: 515-523.

Jian, Y., Dongmei, L., Weixin,J., Hans, U..D., Lan, W. 2013. Effects of Cadmium on Lipid Storage and Metabolism in the Freshwater Crab Sinopotamon henanense. doi: 10.1371/journal.pone.0077569pmcid: pmc379504.

Dhanalakshmi, B. 2016. Biochemical alterations induced byshort and long term exposure of chromium sulphateon the freshwater fish cirrhinus mrigala (ham.) International journal of applied biology and pharmaceutical technology., 8(1): 49 – 56.