HISTOPATHOLOGICAL CHANGES OBSERVED IN THE SPLENIC TISSUES OF THE FISH Clarias batrachus on EXPOSURE TO THE PESTICIDE ROGOR

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NANDINI SHARAN
PAWAN KUMAR

Abstract

Present study focuses on the effect of Rogor induced histological alteration in the spleen tissues of freshwater fish Clarias batrachus more commonly called Mangur. The lethal concentration50 of the pesticide Rogor was calculated which came out to be 4 µl/L. Then, solution of the two sub lethal concentration were prepared in distilled water .The lower and upper sub lethal concentrations were taken as 1 µl/L and 2.5 µl/L respectively. Fishes were grouped in three; first were exposed to lower sub lethal concentration, the second were exposed to higher sub lethal dose and the third were the controlled fishes. Groups consisted of 10 fishes each. Fishers were sacrificed for the experimental protocol on the 5th, 10th and 15th day of exposure to the pesticide. The splenic tissues of fishes from all three groups were dissected and then kept in 10% formalin. They were cut in small pieces and were fixed in neutral formalin solution and light microscopy was performed. The light micrograph was stained with hematoxylin and eosin that showed its parenchymatic components. Seven plates were observed under a low and high power.1st plate was of tissues of controlled fishes. Three plates were of concentration 1 µl/L and three were of 2.5 µl/L each, observed on the 5th, 10th and 15th day of exposure. In controlled fishes under lower and higher power, the red and white pulp enclosed in a capsule, melanomacrophages, distinct trabaculae and blood vessels were observed .In the present work, sub lethal exposure of rogor induced many histopathological anomalies in the spleen, viz. Dissolution of the white pulp, aggregation of more melanomacrophage , autolysis of RBCs  and loss of cellular architecture and matrix and distinct ridge formation. Hence, the experimental protocol displayed the alteration of the normal tissues of spleen of fish thereby showing the toxic impact of the pesticide.

Keywords:
Splenic tissue, Clarias batrachus, Rogor, toxic nature of pesticide.

Article Details

How to Cite
SHARAN, N., & KUMAR, P. (2020). HISTOPATHOLOGICAL CHANGES OBSERVED IN THE SPLENIC TISSUES OF THE FISH Clarias batrachus on EXPOSURE TO THE PESTICIDE ROGOR. UTTAR PRADESH JOURNAL OF ZOOLOGY, 41(15), 1-11. Retrieved from https://mbimph.com/index.php/UPJOZ/article/view/1681
Section
Original Research Article

References

Available:www.fao.org › FAO Home › Fisheries & Aquaculture): Fishery and Aquaculture Country Profiles - The Republic of India

Yeager and Munson; 1945. Article on DDT at www.wikipedia.com

Kulkarni, Rafique. Comparable toxicity study of two heavy metals and pesticides under individual combination exposure on the fish C.puntata. Indian Zoologist. 1992;16(1&2):69-71.

Lin et al. Kim CT, Tsung SH, Peoples JB. Malignant fibrous histiocytoma of ileum . Dis. Colon Rectum. 1983;26:335-338

Cynamid; 1950. Article on dimethoate at www.wikipedia.com

Kumble, Muley. Effect of acute exposure of endosulfan and chloropyrifos on the biochemical composition of the freshwater fish (Sarotherodon mossambicus). Ind. J. Environ. Sci. 2000;4:97-102 .

Scott. Pesticides toxic to vertebrates. Vet. Res. 1967;80:168-173.

Jackson GA. Biological half-life of endrin channel cat fish tissues. Bull. Environ. Contam. Toxicol. 1968; 16:505-507.

Mukesh KN. The effect of pesticides on fish fauna of Bhopal lower lake (M. P.). African Journal of Environmental Science and Technology. 2013;7(7):725-727.

Agius, Roberts. Melano-macrophage centres and their role in fish pathology. J. Fish Dis. 2003;26:499-509

Press C. McL., Dannevig BH, Landsverk T. Immune and enzyme histochemical phenotypes of lymphoid and nonlymphoid cells within the spleen and head kidneyof Atlantic salmon (Salmo salar L.). Fish Shellfish Immunol. 1994;4:79-93.

Hansen JD. Characterization of Rainbow trout terminal deoxynucleotidyl transferase structure and expression. TdT and RAG1 co-expression define the trout primary lymphoid tissues. Immunogenetics. 1997;46:367-375.

Genten, Terwinghe Atlas of fish rainbow trout (Oncorhynchus mykiss). Cell and histology. Science Publishers. 2009;57.

Ellis. Antigen-trapping in the spleen and kidney of the plaice, Pleuronectes platessa (L.). J. Fish Dis. 1980;3:413-426.

Couillard et al. Histopathological evaluation of Atlantic tomcod (Microgadus tomcod) collected at estuarine sites receiving pulp and paper mill effluent. Aquat.Toxicol. 1999;44:263-278.

Wolke REG, GEORGE CJ, Blazer VS. Pigmented macrophage accumulations (MMC, PMB): possible monitors of fish health. In: W.J. Hargis (Editor). Washington D.C., NOAA Technical Report NMFS. 1985;25:93-97.

Montero D, Blazer VS, Socorro J, Izquierdo MS, Tort L. Dietary and culture influences on macrophage aggregate parameters in gilthead seabream (Sparus aurata) juveniles. Aquaculture. 1999;179:523-534.

Fournie et al. Utility of splenic macrophage aggregates as an indicator of fish exposure to degraded environments. J.Aquat. Anim. Health. 2001;13:105-116.

Kurtovic. Histological comparison of spleen and kidney tissue from farmed and wild European sea bass (Dicentrachus labrax L.). Acta adriat. 2008;49(2):147-154. Available:https://teachmephysiology.com/gastrointestinal-system/spleen/function-of-spleen/) : Functions of Spleen.

APHA. Standard methods for the examination of water and wastewater (21st Edn.). Washington, D.C.: American Public Health Association; 2005.

Bucke. Common diseases and parasites of fish in the North Atlantic: Training guide for identification international council for the exploration of the sea techniques in marine environmental sciences, Copenhagen; 1996.

Klontz. Bacterial diseases of the salmonidae in the western United States: Pathogenesis of furunculosis in rainbow trout. Am. J. Vet. Res. 1966;27:1455-1460.

Thorpe JE, Roberts RJ. An aeromonad epidemic in the brown trout. Salmo trutta. L. J. Fish Biol. 1972;4: 441-451.

Zapata and Cooper. Histological and Histochemical Studies on the Early Developmental Stages of the Egyptian Toad Bufo regularis Reuss ; Open Journal of Animal Sciences. 1990;5(2).

Hibiya. Changes in Muscle and Collagen Fibers of Fish after Exposure to Urban Pollutants and Biodegradable Detergents; Microscopy Research. 1982;3(3).

Kaleeswaran et al. Changes in biochemical, histological and specific immune parameters in Catla catla (Ham.) by Cynodon dactylon (L.) Journal of King Saud University-Science. 2010;24(2):139- 152.

Spazier et al. Cytopathology of spleen in eel Anguilla Anguilla exposed to a chemical spill in the Rhine River .Diseases of Aquatic Organisms. 1992;14(1):1-22.