LARVICIDAL ACTIVITY OF CRUDE EXTRACTS AND FRACTIONS OF NATIVE PLANTS AGAINST Aedes aegypti L. AND Culex quinquefasciatus say. (DIPTERA: CULICIDAE)

Main Article Content

S. HANNAH CATHERINE RAJ
N. SHETTU

Abstract

Several millions of people get affected by mosquito-borne sicknesses every year, and hence mosquito-borne sicknesses are regarded as major public health diseases. The vector mosquito Aedes aegypti transmits chikungunya fever, dengue fever, yellow fever viruses and Culex quinquefasciatus transmits filarial nematode and cause filariasis. In the present study, In the present study, the leaves of Cymbopogon citratus, Azadirachta indica, Tagetes erecta, Eucalyptus deglupta and Syzygium aromaticum were screened on Cx. quinquefasciatus and Ae. aegypti mosquito larvae. Ethanol extract of Eucalyptus deglupta leaves was found to be very active and the LC50 and LC90 results were 63.54 ppm, 96.90 ppm against Cx. quinquefasciatus and 72.19 ppm, 137.90 ppm against Ae. aegypti larvae, respectively. Eight fractions were obtained from the active extract and fraction 7 was identified to be very effective. The LC50 and LC90 results of fraction-7 were 5.07 ppm, 12.64 ppm against Cx.  quinquefasciatus larvae and 5.50 ppm, 17.18 ppm against Ae. aegypti larvae, respectively. The active ethanol extract of Eucalyptus deglupta leaves may be used in mosquito control activities.

Keywords:
Culex quinquefasciatus, Aedes agypti, Eucalyptus deglupta, Cymbopogon citrates, Tagetes erecta, Azadirachta indica, Syzygium aromaticum.

Article Details

How to Cite
RAJ, S. H. C., & SHETTU, N. (2020). LARVICIDAL ACTIVITY OF CRUDE EXTRACTS AND FRACTIONS OF NATIVE PLANTS AGAINST Aedes aegypti L. AND Culex quinquefasciatus say. (DIPTERA: CULICIDAE). UTTAR PRADESH JOURNAL OF ZOOLOGY, 41(17), 28-38. Retrieved from https://mbimph.com/index.php/UPJOZ/article/view/1707
Section
Original Research Article

References

Zahran HE, Abdelgaleil SA. Insecticidal and development alinhibitory properties of monoter peneson Culex pipiens L. (Diptera: Culicidae). J. Asia-Pac. Entomol. 2011;14(1):46–51.

Sutthanont N, Choochote W, Tuetun B, Junkum A, Jitpakdi A, Chaithong U, Riyong D, Pitasawat B. Chemical composition and larvicidal activity of edible plant-derived essential oils against thepyrethroid-susceptible and resistant strains of Aedes aegypti (Diptera: Culicidae). J. Vector Ecol. 2010;35:106–115.

Bayen S. Occurrence, bioavailability and toxic effects of tracemetals and organic contaminants in mangrove ecosystems: A review. Environ. Int. 2012;48:84-101.

Chen CD, Nazni WA, Lee HL, Norma-Rashid Y, Lardizabal ML, Sofian-Azirun M. Temephosres istancein field Aedes (Stegomyia) albopictus (Skuse) from Selangor, Malaysia. Trop. Biomed. 2013;30:220– 230.

Chavshin AR, Dabiri F, Vatandoost H, Bavani MM. Susceptibility of Anopheles maculipennis to different class esofinsecticides in West Azarbaijan Province, Northwestern Iran. Asian. Pac. J. Trop. Biomed. 2015;5(5):403–406.

Ruiz-Guerrero R, Rodríguez-Pérez MA, Norzagaray-Campos M. Toxicity of Mexican native plant extracts against larvae of Aedesaegypti (Diptera: Culicidae). Asian Pac. J. Trop. Biomed. 2015;5(4):287–291.

Hayatie L, Biworo A, Suhartono E. Aqueous extracts of seed and peel of Carica Papaya against Aedes aegypti. J. Med. Biol. Eng. 2015; 4(5):417–421.

Pavela R, Benelli G. Ethnobotanical knowledge on botanical repellents employed in the African region against mosquito vectors – a review. Exp. Parasitol. 2016;167C:103–108.

Subashini K, Sivakami R, Jeyasankar A. Larvicidal activity of Scutellaria violacea (Lamiaceae) leaf extracts against three important human vector mosquitoes: Aedesaegypti, Anopheles stephensi and Culex quinquefasciatus (Diptera:Culicidae) from Tamil Nadu, India. Int. J. Mos. Res. 2011;4(2): 108–110.

Ekpenyong CE, Akpan E, Nyoh A. Ethno pharmacology, phyto chemistry, and biological activity of Cymbopogon citratus(DC.) Staff extracts, Chinese J. Nat. Med. 2015;13:321–337.

Chukwuocha UM, Fernández-Rivera O, Legorreta-Herrera M. Exploring the anti malarial potential of whole Cymbopogon citratusplant therapy. J. Ethno pharmacol. 2016;193:517–523.

Avoseh O, Oyedeji O, Rungqu P, Nkeh-Chungag B, Oyedeji A. Cymbopogon species; ethno pharmacology, phyto chemistry and the pharmacological importance, Molecules. 2015;20:7438–7453.

Tarkang PA, Agbor GA, Tsabang N, Tchokouaha RY, Tchamgoue DA, Kemeta D. Effect of long-term oral administration of the aqueous and ethanol leaf extract of Cymbopogon citrates (DC. ex Ness) STAPF, Annals of Biolog. Res. 2012;3(12):5561–5570.

Manvitha K, Bidya B. Review on pharmacological activity of Cymbopogon citrates. Int. J. Herb. Med. 2014;1:5–7.

Chinsembu KC. Plants as antimalarial agents in sub-Saharan Africa, Acta Trop. 2015;152: 32–48.

Costa G, Grangeia H, Figueirinha A, Figueiredo IV, Batista MT. Influence of harvest date and material quality on polyphenolic content and antioxi- dant activity of Cymbopogon citrates infusion, Indust. Crops Prod. 2016;83:738–745.

Okumu FO, Knols BGJ, Fillinger U. Larvicidal effects of neem (Azadirachta indica) oil formulation on the malaria vector Anophelesgambiae. Malaria J. 2007;6:63–68.

Giri RK, Anindya Bose, Subrat Kumar Mishra. Hepato protective Activity of Tageteserecta against carbon tetrachloride-induced hepatic damage in rats. Acta Poloniae Pharmaceutica n Drug Research. 2011;68(6):999-1003.

Farjana Nikkon M, Rowshanul Habib, Zahangir Alam Saud, Rezaul Karim M. (Medicinal plants: Conservation & sustainable use) Efficacy evaluation of Azadirachta indica, Calotropis procera, Datura stramonium and Tagetes erecta against Root-Knot Nematodes Meloidogyne incognita. Pak. J. Bot. 2011;43: 197-204.

Hussain MA, Tariq Mukhtar, Muhammad Zameer Kayani. Combined wound healing activity of Gymnema sylvestere and Tagetes erecta Linn. International Journal of Pharmaceutical Applications. 2011;2(2):135-140.

Farjana Nikkon M, Rowshanul Habib M, Ezaul Karim, Zennat Ferdousi. Insecticidal activity of flower of Tagetes erecta against Tribolium castaneum (Herbst). Research Journal of Agriculture and Biological Sciences. 2009; 5(5):748-753.

Motiur Rahman M, Ekramul Haque M. Tagetes erecta Linn and its mosquitocidal potency against Culex quinquefasciatus. Asian Pacific Journal of Tropical Biomedicine. 2009; 186-188.

Kiplang’at KP, Richard WM. Repellent activities of Ocimum basilicum, Azadirachta indica and Eucalyptus citriodora extracts on rabbit skin against Aedes aegypti. J Entomol Zool Stud. 2013;1:84-91.

Sheshtari MB, Kashani HH, Heidari S, Ghalandari R. Comparative mosquito repellent efficacy of alcoholic extracts and essential oils of different plants against Anopheles stephensi. Afr J Pharm Pharmacol. 2013;7:310-4.

Miyazawa M, Hisama M. Antimutagenic activity of phenylpropanoides from clove (Syzygium aromaticum). J Agric Food Chem. 2003;51(22):6413-22.

Chaieb K, Zmantar T, Ksouri R, Hajlaoui H, Mahdouani K, Abdelly C, Bakhrouf A. Antioxidant properties of essential oil of Eugenia caryophyllata and its antifungal activity against a large number of clinical Candida species. J Mycosis. 2007;50(5):403- 6.

Hussein G, Miyashiro H, Nakamura N, Hattori M, Kakiuchi N, Shimotohno K. Inhibitory effects of Sudanese medical plant extracts on hepatitis C virus (HCV) protease. J Phytother Res. 2000;14:510-6.

Yang YC, Lee SH, Lee WJ, Choi DH, Ahn YJ. Ovicidal and adulticidal effects of Eugenia cryophyllata bud and leaf oil compounds on Pediculus capitis. J Agric Food Chem. 2003; 51(17):4884-8.

World Health Organization. Guidelines for laboratory and field testing of mosquito larvicides. WHO, Geneva; 2005. WHO/CDS/WHOPES/GCDPP/ 13.

Abbott WS. A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 1925;18:265–267.

Rajiv Gandhi M, Daniel Reegan A, Sivasankaran K, Gabriel Paulraj K, Ignacimuthu S. Ovicidal and larvicidal activities of some plant extracts against Aedes aegypti L. and Culex quinquefasciatus Say (Diptera: Culicidae).Asian Pac. J. Trop. Dis. 2016;6(6):468–471.

Aivazi A, Vijayan VA. Larvicidal activity of oak Quercus infectoria Oliv. (Fagaceae) all extracts against Anopheles stephensi Liston. Parasitol. Res. 2009;104:1289–1293.

Yadav R, Srivastava VK, Chandra R, Singh A. Larvicidal activity of latex and stembark of Euphorbia tirucalli plant on the mosquito Culex quinquefasciatus. J. Commun. Dis. 2002;34(4):264–269.

Bansal SK, Singh KV, Kumar S. Larvicidal activity of the extracts from different parts of the plant Solanum xanthocarpum against important mosquito vectors in the arid region. J. Environ. Biol. 2009;30(2):221–226.

Patil SV, Patil CD, Salunkhe RB, Salunke BK. Larvicidal activities of six plants extracts against two mosquito species, Aedes aegypti and Anopheles stephensi. Trop. Biomed. 2010; 27(3):360–365.

Prabhu K, Murugan K, Nareshkumar A, Ramasubramanian N, BragadeeswaranS. Larvicidal and repellent potential of Moringa oleifera against malarial vector, Anopheles stephensi Liston (Insecta: Diptera: Culicidae) AsianPacific J. Trop. Biomed. 2011;124– 129.

Govindarajan M, Sivakumar R. Ovicidal, larvicidal and adulticidal properties of Aspara gusracemosus (Willd.) (Family: Asparagaceae) root extracts against filariasis (Culex quinquefasciatus), dengue (Aedes aegypti) andmalaria (Anopheles stephensi) vector mosquitoes (Diptera: Culicidae) Parasitol. Res. 2014;113:1435–1449.

Manjari MS, Karthi S, Ramkumar G, Muthusamy R, Natarajan D, Shivakumar MS. Chemical composition and larvicidal activity of plant extracts from Clausen adentata (Willd) (Rutaceae) against dengue, malaria, and filariasisvectors. Parasitol. Res. 2014; 113(7):2475–2481.