Published: 2020-12-28

Page: 72-80


Department of Zoology, Yogi Vemana University, YSR District-516005, India.


Department of Zoology, Yogi Vemana University, YSR District-516005, India.

*Author to whom correspondence should be addressed.


The Indian bull frog, Hoplobatrachus tigerinus Daudin was found to be parasitized with the digenean, Tremiorchis ranarum Mehra et Negi. During an investigation of helminth parasites of amphibians from several localities of YSR district, Andhra Pradesh, this parasite was recovered from the intestine of the host. The morphological and diagnostic characteristics of Tremiorchis ranarum was studied by means of light microscopy, scanning electron microscopy along with its pathological effects on the host intestinal tissues to assess the extent of damage caused by them. Histopathogical effects include destruction of intestinal villi, inflammatory fibrosis, inflammation of villi at the site of attachment of parasite, hyperplasia and metaplasia, vacuolation of sub-mucosal cells and degeneration of intestinal layers due to proliferative changes. The pathological effects also include an increase in the thickness and the damage to mucosa.

Keywords: Histopathology, Hoplobatrachus tigerinus, Tremiorchis ranarum, YSR District, metaplasia, hyperplasia.

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De Maynadier PG, Hunter Jr ML. The relationship between forest management and amphibian ecology. A review of the North American literature. Environ Rev. 1995;3:230-261.

Maisam AK, Israa S, Sh A, Ibrahim S. Study prevalence and effect of internal parasites and histopathological changes on common frogs at Baghdad city. Iraq J Agri Sci. 2018;49(3):445-452. Available:10.13140/RG.2.2.25394.86720.

Vié J, Taylor CH, Stuart SN. Wildlife in a Changing World-an Analysis of the 2008 IUCN Red List of Threatened Species. International Union for Conservation of Nature, Gland, Switzerland; 2009.

Williams C. Impact assessment of non native parasites in freshwater fisheries in England and Wales. PhD Thesis, University of Stiring, Scotland; 2007.

Feist SW and Longshaw M. Histopathology of fish parasite infections- Importance for populations. J Fish Biol. 2008;73:2143-2160.

Palmer JM, Meerveld BG. Integrative immunomodulation of gastrointestinal function during enteric parasitism. J. Parasitol. 2001; 87:483-504.

Bose KC, Sinha AK. Histopathology of Clarias attributable to the intestinal cestode Lytocestus indicus. Proc Natl Acad Sci India, Sec B. Biol. Sci. 1983;53:226-230.

Bilqees FM, Fatima H. Histopathology of fish parasites with special reference to fishes of Karachi coast. Proc Parasitol. 1995;19:35-62.

Bilqees FM, Parveen S. Histopathology of the stomach of Cybium guttatum associated with nematode larvae. Proc Parasitol. 1996;22:1-13.

Campos CM, Moraes JRE, Moraes FR. Histopathology of gills of Piaractus mesopotamicus (Holmberg, 1887) and Prochilodus lineatus (Valenciennes, 1836) infested by monogenean and myxosporea, caught in Aquidauana River State of Mato Grosso do Sul, Brazil. Rev Bras Parasitol Vet Jaboticabal. 2001;20(1):67-70.

Benarjee G, Reddy LB. Histopathological and histochemical changes in the liver of Clarias batrachus due to trematode, Euclinostomum heterostomum. J Natcon. 2006a;18(2):251-259.

Benerjee G, Reddy LB. Histopathological and histophysiological changes in the duodenum due to infestation with Djombangia indica. J Curr Sci. 2006b;9(2):647-654.

Nero V, Farwell A, Lister G, VanDer Kraak L EJ, Le T, Van Meer M, Dixon DG. Gill and liver histopathological changes in yellow perch (Perca flavescens) and gold fish (Carassius auratus) exposed to oil sands process-affected water. Ecotox Environ Saf. 2006;63:365– 377.

Camargo MMP, Martinez CBR. Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Neotr Ichth. 2007;5(3):327-336.

Vankara AP, Chikkam V. Histopathological changes of the oesophagus of the freshwater eels, Macrognathus aculeatus (Bl.) and Mastacembelus pancalus (Lac.) due to infection of metacercariae of Clinostomum mastacembeli Jaiswal. 1957. J Par Dis. 2009;33:42-46.

Vankara AP, Chikkam V. Histopathology of heart of freshwater spiny eel, Mastacembelus armatus naturally infected with Tetracotyle metacercaria (Trematoda: Strigeidae). Res J Parasitol. 2013;8(2):45-54. Scialert Journals.

Dap RA. Histopathology of the cestode parasites, Genus Cotugia (Diamare, 1893) from Galus domesticus. UP J Zoo. 2009;29(3):423-426.

Abalaka S, Yakasai FM, Doguwar GBN, Makonjuola KH. Histopathological changes in the gill and skin of adult Clarias gariepinus exposed to ethanolic extract of Parkia biglobosa pods. Bas & Appl Pathol. 2010;3:109–114.

Raissy M, Ansari M. Histopathological changes in the gills of naturally-infected Capoeta aculeata (Cuvier and Valenciennes, 1844) with parasites. Afr J Biotech. 2011;10(68):15422-15425.

Mohammadi F, Mousavi MS, Rezaie A. Histopathological study of parasitic infestation of skin and gill on Oscar (Astronotus ocellatus) and Discus (Symphysodon discus). Aquaculture, Aquarium, Conservation & Legislation. Int J Bioflux Soc. 2012;5(2):88-93.

Padmini MP, Kumar JV. A histopathological study on Gentamycin induced nephrotoxicity in experimental Albino rats. IOSR J Den Med Sci (IOSRJDMS). 2012;1(1):14-17.

Zenon S, Jerzy R, Magdalena K. Histopathological diagnosis in parasitic diseases. Ann Parasitol. 2014;60 (2):127–131.

Fartade AM, Fartade MM. Histopathological study of fresh water fishes infected with ptychobothridean tapeworms parasite from Godavari basin M.S. (India). Int J Res Biosci. 2016;5(1):39-42.

Ozdemir G, Celik ES, Yılmaz S, Gurkan M, Kaya H. Histopathology and blood parameters of bogue fish (Boops boops, Linnaeus 1758) Parasitized by Ceratothoa oestroides (Isopoda: Cymothoidae). Turk J Fisher Aqua Sci. 2016;16:579-590.

Palaq LS, Noorani AI. Histology of gills of Labeo rohita infected by crustacean parasite Argulus sps. Int J Adv Res. 2016;4(7):1387-1392.

Palaq P, Langer S, Ahmad F, Nissa NU. Histopathological alterations in the intestines of Labeo Rohita infected with acanthocephalan parasite, Neoechinorhynchus sp. Biosci Biotech Res Asia. 2017;14(4). Available:

Martin TR, Conn DB. The pathogenicity, localization, and cyst structure of echinostomatid (Trematoda) infecting the kidneys of the frogs Rana clamitans and Rana pipiens. J Parasitol. 1990;76:414-419.

Nieto NC, Camann MA, Foley JE, Reiss JO. Disease associated with integumentary and cloacal parasites in tadpoles of northern red-legged frog Rana aurora aurora. Dis Aqu Org. 2007;78(1):61-71.

Miller DL, Schrecengost J, Merrill A, Kilgo J, Ray HS, Miller KV, Baldwin CA. Hematology, parasitology, and serology of free-ranging coyotes (Canis latrans) from South Caolina. J Wildl Dis. 2009;45:863-869.

Al-Attar AM. Hematological, biochemical and histopathological studies on Marsh frog, Rana ridibunda, naturally infected with Waltonella duboisi. Int J Zool Res; 2010. ISSN 1811-9778.

Molnar K. Redescription and histopathology of Myxobolus cyprinicola Reuss, 1906, an intestinal parasite of the Common Carp (Cyprinus carpio L.). Acta Protozool. 2002;41:279–283.

Reddy LB, Benarjee G, Rajender G, Bikshapthi V, Swami M. Histopathological and histochemical abnormalities induced Euclinostomum heterostomum in the liver of fresh water fish Channa punctatus. J Aqua Biol. 2006;21(2):263-267.

Patil SD, Chaudhari HW. Histopathological studies on intestine of Columba livia Gmellin, 1789 infected with cestode parasites. Env Conser Jour. 2010;11(1&2):71-73.

Reddy LB, Benarjee G. Histopathology of intestine of trematode helminth infected fish. Int J Curr Microbiol App Sci. 2014;3(2):330-334.

Hiware CJ, Jadhav BV, Mohekar AD. Applied Parasitology. A practical manual. Mangaldeep Publications; 2003.

Yamaguti S. Systema helminhum, Vol-I. The digenetic trematodes of Vertebrates. Interscience Publishers Inc., New York; 1958.

Mehra HR, Negi PS. On a new trematode Tremiorchis ranarum nov. gen. nov. sp. from common Indian frog, Rana tigrina. Parasitol. 1926;18:168-181.

Bhalerao GD. On the Trematodes of the digestive tract of a common Indian Frog, Rana tigrina, with a descrpition of Centrovitus pentadelphi n.g., n.sp. Parasitol. 1926;18(02).

Verma SC. On the synonymy of the Genera Tremiorchis Mehra and Negi 1926 and Centrovitus Bhalerao 1926, with the descrpition of Tremiorchis ranarum n.sp. Parasitol. 1930;22 (03).

Singh KS. Some trematodes col1ected in India. Trans Amer Micr Soc, Menasha. 1954;73(2):202-210.

Bhardwaj ON. Studies on the intraspecific variations of Tremiorchis ranarum Mehra et Negi, 1926, Plagiorchiidae Luhe, emert Ward, 1917, Trematoda and discussion on causes and effects of intraspecific variations. Proc Nat Acad Sci. 1967;32:445-450.

Agarwal V. Studies on some trematodes of frogs from Lucknow. Ind J Helminthol. 1966;28(1):82-90.

Ali SM, Karyakarte PP. Observations on developmental stages of Tremiorchis ranarum Mehra et Negi, 1926 (Trematoda: Plagiorchiidae). Marath Univ J Sci. 1970;9:161-168.

Dwivedi MP, Chauhan BS. On some digenetic trematodes. Part-II. J Zool Soc India. 1970;22:87-96.

Mukherjee RP, Ghosh RK. Studies on some amphibian trematodes from Uttar Pradesh and West Bengal (Part I). Ind J Helminth. 1970;22(1):61-78.

Sinha A, Sahay U. On the occurrence of Tremiorchis ranarum Mehra et Negi, 1926 from Rana cyanophylyctis at Patna. Indian. J Helminthol. 1971;23: 56-69.

Pandey KC. Studies on some known trematode parasites. Ind J Zool. 1973;14:192-219.

Pandey KC, Agarwal N. Further remarks on the genus Tremiorchis Mehra et Negi. 1925. Proc Ind Acad Sci. 1981;90(5):567-570.

Pandey D, Pandey JP, Shukla N. Morphological intraspecific variations of Tremiorchis ranarum of Rana tigrina in District Sidhi (M.P) India. Int J Sci Res Publ. 2013;3(5):1-3.

Boon MV, Kok LV. Microwave cookbook of pathology: the art of microscopic visualization. Coulomb press. Leiden. 1992;211.

Login GR, Dvorak. Methods of microwave fixation for microscopy. A Rev Res Clin Applic, 1970-1992; 1994.

Pressnell JKGL, Humason, Schreibman M. Humans animal tissue techniques, 5th Ed. Johns Hopkins Univ. press, Baltimore; 1997.

Ouellet M, Mikaelian I, Pauli BD, Rodrigue J, Green DM. Historical evidence of widespread chytrid infection in North America amphibian populations. Conserv Biol. 2005;19: 1431−1440.

Khatoon N. Histopathologic alterations associated with Syphacia sp. (Nematode) in the intestine of Nesokia indica. Turk J Zool. 2005;28:345-351.

Benarjee G, Reddy LB. Pathobiological and histochemical changes on liver of the fresh water murrel infected with trematode parasite. J Ecotoxicol Environ Monit. 2008;18(6):565-572.

Bamidele A. Histopathological study on the parasitised visceral organs of some fishes of Lekki Lagon, Lagos, Nigeria. Lif Sci Journ. 2007;4(3):70-76.

Hossain MK, Hossain MD, Rahman MH. Histopathology of some diseased fishes. J Lif Eart Sci. 2007;2(2):47-50.

Kurpad RRK, Shuba S, Muddegowda HP, Lingegowda BJ. The common and uncommon cestodal infestation encountered in routine histo-pathological practice from a semi-urban population in south India and their public health importance. Int J Curr Sci Res. 2011;1(2):48-51.

Pardeshi P, Hiware J, Wangswad C. Histopathology of the liver of Mastacembelus armatus (Lacepede, 1800) due to trematode parasite, Allocreadium khami n.sp. J Par Dis. 2012;36:53-5. DOI:10.1007/s12639-011-0091-y.

Rezaei S, Pazooki J, Sharifpour I, Masoumian M. Histopathological observations in Neogobius bathybius (Actinopterygii: Gobiidae) infected by Dichelyne minutus (Nematoda: Cucullanidae) in the Caspian Sea, Iran. Turk J Zool. 2013;37:329-333.

Shareef PAA, Syed MAA. Studies on the epidemiology and histopathology of Euclinostomum heterostomum (Trematoda: Digenea) infection in Channa punctata from North India. Arch Pol Fish. 2015;23:133- 140.

Vankara AP. Mode of attachment and Pathogenecity of Circumonchobothrium shindei (Eucestoda: Ptychobothriidae) in Mastacembelus armatus of River Godavari, Andhra Pradesh, India. Int J Zool Res. 2018;14(1):1-7.