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The present study has been conducted to understand the effect of sublethal concentration of arsenic (96 LC50 – 10% SLC) on the biochemical composition and histology of muscle of the milk fish Chanoschanos for a period of 10, 20 and 30 days. Glycogen, protein and lipids were measured both in control and experimental fish. During the exposure periods the biochemical constituents (carbohydrates, proteins and lipids) were decreased in the experimental fish with duration. The histopathological changes induced in the muscle were degeneration of muscle fibre, oedema, atrophy of muscle fibres and shortening of muscle bundle.
Sehar Afshan, Shafaqat Ali, UzmaShaista Ameen, Mujahid Farid, Saima Aslam Bharwana, Fakhir Hannan, Rehan Ahamad. Effect of different heavy metal pollution on fish. Res. J. Chem.Env. Sci. 2014;2(1):74-79.
Bedii Cicik, Kenan Engin. The effects of cadmium on levels of glucose in serum and glycogen reserves in the liver and muscle tissues of Cyprinus carpio (L. 1758). Turk. J. Vet. Anim. Sci. 2005;29:113-117.
Bhilave MP, Muley DV, Deshpande VY. Biochemical changes in the fish Cirrhinus mrigala after acute and chronic exposure of heavy metals. Nature Environment and Pollution Technology. 2008;7(1):65-71.
Mehjbeen Javed, Nazura Usmani. Assesment of heavy metal (Cu, Ni, Fe, Co, Mn, Cr, Zn) pollution in effluent dominated rivulet water and their effect on glycogen metabolism and histology of Mastacembulus armatus. Springer Plus. 2013;2(390):1-13.
Dhanalakshmi B. Biochemical alterations induced by short and long term exposure of chromium sulphate on the freshwater fish Cirrhinus mrigala (Ham.). International Journal of Applied Biology and Pharmaceutical Technology. 2017;8(1):49-56.
Ashok Kumar Verma, Sadguru Prakash. Effect of arsenic on enzyme activity of a fresh water fish Mystus vittatus. International Journal of Fisheries and Aquatic Studies. 2020;8(3):28-31.
Sabrina Barillet, Valerie Larno, MagaliFloriani, Alain Devaux, Christelle Adam-Guillermin. Ultrastructural effects on gill, muscle and gonadal tissues induced in zebra fish (Danio rerio) by a waterborne uranium exposure. Aquatic Toxicology. 2010;100(3):295-302.
Gonca Alak, Muhammed Atamanalp, Ahmet Topal, Harun Arslan, Esat Mahmut Kocaman, Ertan Oruc. Effect of sublethal lead toxicity on the histopathology and antioxidant enzyme activity of rainbow trout (Oncorhynchus mykiss). Fresenius Environmental Bulletin. 2013;22(3):733-738.
Celine Hilda Marry S, Bhuvaneswari D, Anandan R. Biochemical and histopathological studies on lead nitrate induced toxicity in freshwater fish grass carp (Ctenopharynqodon idella). European Journal of Experimental Biology. 2015;5(11):24-30.
Saravpreet Kaur, Kuldeep Singh Khera, Jasjit Kaur Kondal. Heavy metal induced histopathological alterations in liver, muscle and kidney of freshwater cyprinid, Labeorohita (Hamilton). Journal of Entomology and Zoology Studies. 2018a;6(2):2137-2144.
Finney DJ. Probit Analysis, Cambridge University Press, London; 1971.
Sprague JB. The ABC’s of pollutant bioassays using fish. In: Biological methods for the assessment of water quality. ASTM STP. 1973;(528):6-30.
Kemp A, Andrienne JM. Kits Van Heijningen. A colorimetric method for the determination of glycogen in tissues. J. Biol. Chem. 1954;56(4): 646-648.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J. Biol. Chem. 1951;193:265-275.
Folch J, Lees M, Sloane Stanley SH. A simple method for isolation and purification of total lipids from animal tissues. J. Biol. Chem. 1957;226(1):497-507.
Gurr E. Methods of analytical histology and Histochemistry. Leonard Hill Ltd., London. 1959;45-49.
Srinivasa Naik Banavathu, Jagadish Naik Mude. Heavy metal mercuric chloride (HgCl2) induced biochemical changes in the Indian major carp Labeorohita (Hamilton). Trends in Fisheries Research. 2018;7(3):65-71.
Rama Srivastava, Neera Srivastava. Changes in nutritive value of fish, Channa punctatus after chronic exposure to zinc. Journal of Environmental Biology. 2008;29(3):229-302.
Cappon ID, Nicholls DM. Factors involved in increased protein synthesis in liver microsomes after administration of DDT. Pesticide Biochemistry and Physiology. 1975;5(2):109-118.
Pazhanisamy K, Indra N. Toxic effects of arsenic on protein content in the fish, Labeorohita (Hamilton). Nature Environment and Pollution Technology. 2003;6(1):113- 116.
Akhter Ali Siddiqui, Suponglemla Chang. Cadmium chloride intoxication and evaluation of protein changes in Clarias batrachus (Linn). International Journal of Current Microbiology and Applied Sciences. 2014;3(1):787-794.
Muneesh Kumar, Anchal Raj, Rajesh Kumar, Lekh Raj. Estimation of glycogen and protein contents of certain tissues of freshwater fish, Clarias batrachus (Linn) after exposure of zinc sulphate. Int. J. Adv. Multidiscip. Res. 2017; 4(1):33-41.
Emad H. Abou EL-Naga, Khalid M. El-Moselhy and Mohamed A. Hamed. Toxicity of cadmium and copper and their effect on some biochemical parameters of marine fish Mugilseheli. Egyptian Journal of Aquatic Research. 2005;31(2):60-71.
Satyendra Singh, Srivastava AK. Sublethal effects of copper and arsenic on some biochemical and hematological parameters of the blood in an air breathing fish Heteropneustes fossilis. J. Ecophysiol. Occup. Hlth. 2015;15(3&4):105-114.
Jagadeshwarlu R, Sunitha Devi G. Effect of sublethal copper exposure on glycogen, glucose and total lipid levels in (muscle and liver) fish, Oreochromis mossambicus (Peters). International Journal of Zoological Studies. 2018;3(1):123-127.
Sadguru Prakash, Ashok Kumar Verma. Effect of arsenic on lipid metabolism of a fresh water cat fish, Mystusvitatus. Journal of Fisheries and Life Sciences. 2019;4(1):33-35.
Kaoud HA, El-Dahshan AR. Bioaccumulation and histopathological alterations of the heavy metals in Oreochromis niloticus fish. Nature and Science. 2010;8(4):147-156.
Bharat Bhusan Patnaik, Hongray Hourella J, Theresia Mathews, Selvanayagam M. Histopathology of gill, liver, muscle and brain of Cyprinus carpio communis L. exposed to sublethal concentration of lead and cadmium. African Journal of Biotechnology. 2011;12218-12223.
Bhuvaneshwari R, Padmanaban K, Babu Rajendran R. Histopathological alterations in muscle, liver and gill tissues of zebra fish Danio rerio due to environmentally relevant concentrations of organochlorine pesticides (OCPS) and heavy metals. Int. J. Environ. Res. 2015;9(4):1365-1372.
Saravpreet Kaur, Kuldeep Singh Khera, Jasjit Kaur Kondal. Effect of water contaminated with heavy metals on histopathology of freshwater catfish, Clarias batrachus. International Journal of Chemical Studies. 2018b;6(4):3103-3108.