A STUDY ON THE EFFICIENCY OF PHYLLANTHUS NIRURI IN AMELIORATING THE DRUG INDUCED OXIDATIVE STRESS IN Oreochromis mossambicus AND ITS COMPARISON WITH A HERBAL FORMULA LIV 52

Main Article Content

L. P. REMA
http://orcid.org/0000-0003-2402-5563

Abstract

Hepatoprotective and antioxidant potential of Phyllanthus niruri and Liv.52, in relieving the paracetamol induced hepatotoxicity in Oreochromis mossambicus is compared. Exposure of fish to different concentrations of Paracetamol showed increase in TBA value and a decrease in the total protein content. Phyllanthus niruri and Liv.52 supplied through feed probably decreases the reactive oxygen species (ROS) resulting in lower TBA value indicating reduction in lipid peroxidation. Lower protein level in these groups may be due to enhanced utilization of proteins for active metabolism and growth. Fish fed with Phyllanthus niruri showed lower TBA value than the paracetamol exposed animals, but the fish fed with commercially produced Liv.52 showed even lower TBA value than those fed with Phyllanthus niruri, indicating the greater protective effect of commercially available drug Liv.52 which is a combination of many medicinal plants. The data are statistically analysed using t test.

Keywords:
Hepatotoxicity, ROS, Phyllanthus niruri, Liv 52

Article Details

How to Cite
REMA, L. P. (2021). A STUDY ON THE EFFICIENCY OF PHYLLANTHUS NIRURI IN AMELIORATING THE DRUG INDUCED OXIDATIVE STRESS IN Oreochromis mossambicus AND ITS COMPARISON WITH A HERBAL FORMULA LIV 52. UTTAR PRADESH JOURNAL OF ZOOLOGY, 42(5), 48-55. Retrieved from https://mbimph.com/index.php/UPJOZ/article/view/1991
Section
Original Research Article

References

Moore MN. Cellular responses to pollutants. Mar Pollut Bull. 1985;13:42-43.
DOI.org/10.1016/0025-326X(85)90003-7

Roch M, Mc Caster JA, Matheson AT, Clark MJR, Olafson RW. Hepatic metallothionien in rainbow trom (Salmo Gairdneri) An Indicator of Metal Pollution In the Campbell River System: Can. J. fish Aquat. Sci. 1982;39:1596-1601.

Slater TF. Biochemical studies on liver injury. In Biochemical Mechanisms of Liver Injury. T.F. Slater, ed. 1978;2-44.

Moore MN. Cytochemical determination of cellular responses to environmental stressors in marine organisms. In Biological Effects of Marine Pollution and the Problem of Monitoring Organisms, A.D. Mc Intyre and J.B Pearce, eds. 1980;179:7-15.

Viarengo A. Biochemical effects of trace metals. Marine Pollution Bulletin. 1985;16:4.
Available:https://doi.org/10.1016/0025-326X(85)90006-2

Kala Suhas Kulkarni, Md. Rafiq, Gopumadhavan S, Venkataranganna MV, Madhumathi BG, Mitra SK. Protective effect of Liv.52 on Na+ -K+ -ATPase activity in Paracetamol-induced Hepatotoxicity, Medicine Update. 2002;10(5):53-56.

Rao YS. Experimental production of liver damage and its protection with Phyllanthus niruri and Capparis spinosa in white albino rats. 1985;2,117.

Prasad GC. Effect of Liv 52 on the liver in vitro. J. Res. Ind. Med. 1975;4:15.

Karandikar SM, Joglekar GV, Chitale GK, Balwani JH. Protection by indigenous drugs against hepatotoxic effects of carbon tetrachloride ‐ a long term study. Acta pharmacol et. toxicol. 1963;20:274-280.
Available:https://doi.org/10.1111/j.1600- 0773.1963.tb01745.x

Patel JR, Sara NL. Effect of Liv.52 on structural and functional damage caused by some hepatotoxic agents. Probe. 1963;10:19.

Rathore HS, Rawat H. Liv.52 protection against Cd induced histomorphological changes in mouse spleen, duodenum and small intestine, Ind. Drugs. 1989;26:10,533.

Suresh Kothari, Bhaskar Reddy, P, Rathore HS. Protective role of Liv.52 against histological damage due to CdCl2 Toxicity in the Intestine of Teleost Fish. Probe: 1990; 3(XXIX):220-228.

Nahid Tabassum, Sushma Chathurvedi, Aggrawal SS, Nissar Ahmed. Hepatoprotective studies on phyllanthus niruri on paracetamol induced liver cell damage in albino mice. Experimental Medicine. 2005;12:211-212.

Dwivedi SK, Sharma MC, Mukherjee SC, Lal I, Pandey NN. Comparative efficiency of Liv.52 & Andrographis paniculata Nees in experimental liver damage in rabbits. Indian Drugs. 1987;25:1-4.

Susan Eapen, D'souza SF. Prospects of Genetic Engineering of Plants For Phytoremediation of Toxic Metals; 2004.
DOI: 10.1016/j.biotechadv.2004.10.001

Agrawal SS, Garg A, Agrawal S. Screening of Phyllanthus niruri Linn and Ricinus communis Linn on alcohol induced liver cell damage in non-Hepatectomized and partially hepatectomized rats. Ind. J. Pharm. 1986;18:211-214.

Warvedkhar VS, Saslaw LD. J Biol Chem. 1959;234.

Anup Bhaumik, Sharma MC. Therapeutic efficacy of Liv.52 in paracetamol induced hepatopathy in rabbits. Indian Journal Veterinary Research. 2002;11(1):8-17.
Available:https://doi.org/10.1080/09712119.1993.9705965

Viarengo A,Canes L, Pertica M, Poli G, Moore MN, Oricinesu M. Heavy metal effects in lipid peroxidation in the tissue of Mytilus galloprovincialis Lam, Comp. Biochem. Physiol. C. 1990;97C:(1):37-42.

Knopowski J, Wieczorowska-Tobis K, Gutowski J. Pathophysiology of Aging. J. of Physiology and Pharmacology. 2002; 52(2):135-146.

Martin GM, Austad SN, Johnson TE. Genetic analysis of aging: role of oxidative damage and environmental stresses. Nature Genetics. 1996;13:25-34.
Available:https://doi.org/10.1038/ng0596-25

Berlett SS, Stadtman ER. Protein oxidation in Aging, Disease and Oxidative stress. J. of Biological Chemistry. 1997;272.
Available:https://doi.org/10.1074/jbc.272.33.20313

Finkel T, Holbrook NJ. Oxidant, oxidative stresses and biology of Aging. Nature. 2000;408:329-247.
Available:https://doi.org/10.1038/35041687.

George SG, Lombs TL, Pirie BJS. Characterization of metal containing granules from the kidney of the common mussel. Mytilus Edulis; 1982.

Metwally MAA. Effects of garlic (Allium Sativum) on some heavy metal copper and zinc induced alteration in serum lipid profile of Oreochromis niloticus. World J. of Fish and Marine Science; 2009.

Sharmila Banu G, Kumar G, Murugesan AG. Effects of leaves extracts of Ocimum Sanctum on Arsenic Induced Toxicity In Wistar albino Rats; 2010.
DOI: 10.1016/j.fct.2008.12.004

Sankar G, Elavarasi A, Sakkaravarthi K, Ramamoorthy K. Biochemical changes and growth performance of black tiger shrimp larva after using Ricinus Communis Extract As Feed Additive. Int.J.of Pharm Tech Research. 2011;3:1.201-208.

Ezzat MI, Okba MM, Ahmed SH, El-Banna HA, Prince A, Mohamed SO, Ezzat SM. In-depth hepatoprotective mechanistic study of Phyllanthus niruri: In vitro and in vivo studies and its chemical characterization. PLoS One. 2020;15(1):e0226185.
DOI:10.1371/journal.pone.0226185. eCollection 2020.PMID: 31940365