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The present study aimed to know the impact of iron oxide nanoparticles treated tannery effluent on hematological, enzymatic, and biochemical characteristics of Tilapia Oreochromis mossambicus. Iron oxide nanoparticles were synthesized and characterized by using Scanning Electron Microscope, Energy Dispersive X-Ray Spectroscopy, X-Ray Powder Diffraction, Fourier Transform Infrared Spectroscopy, and Vibrating Sample Magnetizer. Physico-chemical characteristics of tannery effluent were estimated.  Dissimilar strength of Iron oxide nanoparticles such as 50,100,150,200 and 250 ppm of Fe3O4NPs were used for treating tannery effluent. For median lethal (LC50) studies dissimilar strength of iron oxide nanoparticles treated tannery effluent such as 200,225 and 250 ppm were used and seven fishes were maintained in triplicates for 96 hrs. Based on medium lethal studies, 0 (control), 2.32 (low), 4.65 (medium), and 23.27ppm (high) were selected for sub-lethal tests by introducing Tilapia for 14 days. At the end of the 14th day of exposure, fish and blood samples were collected randomly in each concentration along with control for further test such as hematology, enzymes (Aminotransferase, Alanine aminotransferase), and biochemical analysis in muscle, gill, and liver of tilapia. The results indicated that 200 and 250 ppm iron oxide nanoparticles were effective to reduce the toxic substances of tannery effluent. Hematological parameters and enzymatic parameters (Aminotransferase (AST) and Alanine aminotransferase (ALT) of tilapia fish exposed to sub-lethal concentration of Fe3O4 Nps are increased in T1 and T2.  Biochemical characteristics such as protein, carbohydrate, and lipid in muscle, gill, and liver of Tilapia are higher in T1.

Biochemical, enzymatic, hematological, iron oxide nanoparticles, tannery effluent, tilapia

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Leslie La Conte, Nitin Nitin, Gang Bao. Magnetic nanoparticle probes. Materials Today. 2015;8(5):32-38.

Erkan Can, Volkan Kizak, Murathan Kayim, Safak Seyhaneyildiz Can, Banu Kutlu, Mehmet Ates, et al. Nanotechnological applications in aquaculture-seafood industries and adverse effects of nanoparticles on environment. J. of Mat. Sci. Engineer. 2011;5:605- 609.

Prasad KS, Pathak D, Patel A, Dalwadi P, Prasad R, Patel P, et al. Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect. African Journal of Biotechnology. 2011; 10(41):8122-8130.

Crane RA, Scott TB. Nanoscale zero-valent iron: future prospects for an emerging water treatment technology. Journal of Hazardous Materials. 2012;211-212:112 - 125.

Lkhagvadulam B, Tsagaantsetseg B, Tergel D, Chuluunkhuyag S. Removal of chromium from a tannery wastewater by using a maghemite nanoparticles. International Journal of Environmental Science and Development. 2017;8(10):696-702.

Shaw BJ, Handy RD. Physiological effects of nanoparticles on fish: A comparison of nanometals versus metal ions. Environ. Int. 2011;37:1083-1097.

Sarikaya R, Yilmaz M. Investigation of acute toxicity and the effect of 2,4-D (2,4-dichlorophenoxyacetic acid) herbicide on the behavior of the common carp Cyprinus carpio L 1758; Pisces, Cyprinidae. Chemosphere. 2003;52(1):195-201.

Sevcikova M, Modra H, Blahova J, Dobsikova R, Plhalova L, Zitka O, et al.. Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copper. Vet. Med. 2016;61(1):35-50.

Ates M, Demir V, Arslan Z, Kaya H, Yılmaz S, Camas M. Chronic exposure of tilapia (Oreochromis niloticus) to iron oxide nanoparticles: Effects of particle morphology on accumulation, elimination, hematology and immune responses. Aquat. Toxicol. 2016;177: 22-32.

Bahmani M, Kazemi R, Donskaya P. A comparative study of some hematological features in young reared sturgeons Acipenser persicus and Huso huso. Fish Physiol. Biochem. 2012;4(2):135–140.

Jahanbakhshi A, Hedayati A. The effect of water soluble fraction of crude oil on serum biochemical changes in the great sturgeon Huso huso. Comp. Clin. Pathol. 2013;22(6): 1099-1102.

APHA: Standard methods for examination of water and wastewater. 22nd ed. Washington: American Public Health Association, USA; 2012.

OECD. Guidelines for the testing of chemicals. Fish, Acute Toxicity Test. Organization for Economic Cooperation and Development, Paris, France. 1992;203.

Stevens ML. Fundamentals of clinical hematology. WB Saunders, Philadel. 1997; 1-392.

Richard Lee, John Foerster, John Lukens, Frixos Paraskevas, John P Greer, George M. Rodgers. (Eds) Wintrobe’s Clinical Hematology. Hematol. Oncol. 1998;17(2): 84–84.

Nelson DA, Morris MW. Basic methodology, hematology and coagulation, Clinical diagnosis and management by laboratory methods. 17th ed. Philadel., WB Saunders. 1989;578–625.

Reitmans S, Frankel S. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 1957;28: 53-56.

Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin Phenol Reagent. J. Biol. Chem. 1951;193:265-275.

Carrol NV, Longley RW, Roe JH. Glycogen determination in liver and muscle by use of anthrone reagent. J. Biol. Chem. 1956;22:583-593.

Folch J, Lees M, Stanley SGH. A simple method for the isolation and purification of total lipids from animal tissues. J. of Biochem. 1957;226:497–509.

Ali A, Zafer H, Zia M, Hag IU, Phull AR, Ali JS, et al. Synthesis, characterization, application and challenges of iron oxide nanoparticles. Nanotech. Sci. and Appl. 2016;9:49-67.

Harini PL, Muhammad F, R Marsi, D Setiabudidaya. Synthesis and properties of Fe3O4 nanoparticles by co- precipitation methods to removel procin dye. Internat. J. of Envir. Sci. and Envir. 2013;4:40-46.

Bumbin A, Borechbiel MW, Choyke L, Fugger Eggeman. Synthesis and Characterization of Ultra-small Super Paramagnetic Iron Oxide Nanoparticles Thinly Coated with Nanotechnology. 2008;19(33):33560.

Keerthika V, Ramesh R, Rajan MR. Toxicity assessment of iron oxide nanoparticles in Labeo rohita: Internat. J. of Fish. and Aqua. Stud. 2017;5(4): 01-06.

Rouhollah Khodadust, Goza Unsoy, Serap Yalcm, Gungor Gunduz, Ufuk Gunduz Pamam. Dendrimer- coated Iron oxide nanoparticles: Synthesis and characterization of different generations. J Nanopart. Res. 2013;15:1488.

Chen TH, Lin CC, Meng PJ. Zinc oxide nanoparticles alter hatching and larval locomotor activity in zebra fish (Danio reio). J. of Haz. Mat. 2014;277:134-140.

Wang Y, Parvin Kaur. Augustine Tuck Lee Tan. Iron oxide magnetic nanoparticles synthesis by atmospheric microplasmas. Plasma and Appli. 2013;32:1460343.

Muthukkaruppan M, Parthiban P. A study on the physicochemical characteristics of tannery effluent collected from Chennai. International Research Journal of Engineering and Technology (IRJET). 2018;5(3):24-28.

Kataria HO, Jain OP. Physico-chemical analysis of river Ajnar. Indian Journal of Environmental Protection.1995;12(9):6467.

Poole NJ, Wildish DJ, Kristmanson. The effects of paper industry on the aquatic Reviews on Environmental Contamination and Toxicology.1978;8:153.

Rajan MR, Murali SR. Dechloridation of treated tannery effluent using leaves of plants. Nature Envir, and Poll. Tech. 2011;4:573-578.

Shahida Parveen, Ram Bharose, Dharam Singh. Assessment of physico-chemical properties of tannery waste water and its impact on fresh water quality. Internat. J. of Current Microbiol. and Appl. Sci. 2017;6(4): 1879-1887.

Taju G, Abdul Majeed S, Nambi KSN, Sarath Babu V, Vimal S, Kamatchiammal S, Sahul Hameed AS. Comparison of in-vitro and in vivo Acute Toxicity assays in Etroplus suratensis and three cell lines in Relation to Tannery Effluent: Chemosphere. 2004;87:55- 61.

Anulipi Aich, Abhishek Roy Goswami, Utpal Singh Roy, Suphra Kumar Mukhopadhyay. Ecotoxicological assessment of tannery effluent using guppy fish (Poecilia reticulate) as an Experimental model and Biomarker study: J. of Toxi. and Envir. Hlth. 2015;37-41.

Saravanan D, Gomathi T, Sudha PN. Sorption studies on heavy metal removal using chitin/bentonite biocomposite. International Journal of Biological Macromolecules. 2013; 53:67-71.

Sivakami MS, Gomathi T, Venkatesan J, Jeong HS, Kim SK, Sudha PN. Preparation and characterization of nanochitosan for treatment. International Journal of Biological Macromolecules. 2013;57: 204-212.

Shaluei F, Hedayati A, Jahanbakhshi A, Kolangi H, Fotovat M. Efffect of subacute exposure to Silver nanoparticle on hematological and plasma biochemical indices in Silver carp (Hypophythalmichtys molitrix. Human and Experimental Toxicology. 2013; 32(120:1-8

Remya AS, Ramesh M, Saravanan M, Poopal RK, Bharathi S, Nataraj D. Iron oxide nanoparticles to an Indian major carp, Labeo rohita: Impacts on hematology, iono regulation and gill Na+/K+ ATPase activity. J. of King Saud Univ. Sci. 2014;27(2):1018-3647.

Rajan MR, Archana J, Ramesh R, Keerthika V. Toxicity of zinc oxide nanoparticles in tilapia Oreochromis mossambicus. Paripex – Ind. J. of Res. 2016;5(10):220- 224.

Behera T, Swain P, Rangacharulu PV, Samanta M. Nano-Fe as feed additive improves the hematological and immunological parameters of fish, Labeo rohita H. Appl Nanosci. 2014;4(6):687–694.

Ashouri S, Keyvanshokooh S, Salati AP, Johari SA, Pasha-Zanoosi H. Effects of different levels of dietary selenium nanoparticles on growth performance, muscle composition, blood biochemical profiles and antioxidant status of common carp (Cyprinus carpio). Aquaculture. 2015;446:25–29.

Abdel-Khalek AA, Badran SR, Marie MAS. Toxicity evaluation of copper oxide bulk and nanoparticles in Nile tilapia, Oreochromis niloticus, using hematological, bioaccumulation and histological biomarkers. Fish Physiol. Biochem. 2016; 42(4):1225-1236.

Suganthi P, Murali M, Sadiq Bukhari A, Syed Mohamed HE, Basu H, Singhal RK. Behavioural and histological variations in Oreochromis mossambicus after exposure to ZnO nanoparticles. Int. J. of Appl. Res. 2015;1(8):524-531.

Ramesh M, Sankaran M, Veera-Gowtham V, Krishnan PR. Hematological, biochemical and enzymological responses in an Indian major carp Labeo rohita induced by sublethal concentration of waterborne selenite exposure. Chem. Biol. Interact. 2014;207:67-73.

Firat O, Cogun HY, Yüzereroğlu TA, Gök G, Firat O, Kargin F,Kötemen Y. A comparative study on the effects of a pesticide (Cypermethrin) and two metals (copper, lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus. Fish Physiol. Biochem. 2011;37:657-666.

Ferat O, Kargen F. Individual and combined effects of heavy metals on serum biochemistry of Nile Tilapia, Oreochromis niloticus. Arch. Environ. Contam. Toxicol. 2010;58:151-157.

Hao L, Chen L, Hao J, Zhong N. Bioaccumulation and sub-acute toxicity of zinc oxide nanoparticles in juvenile carp (Cyprinus carpio): A comparative study with its bulk counterparts. Ecotoxicol. Environ. Saf. 2013; 91:52- 60.

Savorelli F, Manfra L, Croppo M, Tornambè A, Palazzi D, Canepa S, et al. Fitness evaluation of Ruditapes philippinarum exposed to Ni. Biol. Trace Ele. Res. 2017;177(2):384-393.

Vinodhini R, Narayanan M. Effect of heavy metals on the level of vitamin E, total lipid and glycogen reserves in the liver of common carp (Cyprinus carpio L.). Maejo Int. J. Sci. Technol. 2008;2:391-399.

Sivakumar P, Kanagappan M, Sam Manohar Das. Flux of tissue substrates in Danio rerio exposed to raw tannery effluent. Internat. J. of Engin. Res. and Gen. Sci.2015;3(6):496-502.