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The present study aimed to know the impact of iron oxide nanoparticles treated tannery effluent on hematological, enzymatic, and biochemical characteristics of Tilapia Oreochromis mossambicus. Iron oxide nanoparticles were synthesized and characterized by using Scanning Electron Microscope, Energy Dispersive X-Ray Spectroscopy, X-Ray Powder Diffraction, Fourier Transform Infrared Spectroscopy, and Vibrating Sample Magnetizer. Physico-chemical characteristics of tannery effluent were estimated. Dissimilar strength of Iron oxide nanoparticles such as 50,100,150,200 and 250 ppm of Fe3O4NPs were used for treating tannery effluent. For median lethal (LC50) studies dissimilar strength of iron oxide nanoparticles treated tannery effluent such as 200,225 and 250 ppm were used and seven fishes were maintained in triplicates for 96 hrs. Based on medium lethal studies, 0 (control), 2.32 (low), 4.65 (medium), and 23.27ppm (high) were selected for sub-lethal tests by introducing Tilapia for 14 days. At the end of the 14th day of exposure, fish and blood samples were collected randomly in each concentration along with control for further test such as hematology, enzymes (Aminotransferase, Alanine aminotransferase), and biochemical analysis in muscle, gill, and liver of tilapia. The results indicated that 200 and 250 ppm iron oxide nanoparticles were effective to reduce the toxic substances of tannery effluent. Hematological parameters and enzymatic parameters (Aminotransferase (AST) and Alanine aminotransferase (ALT) of tilapia fish exposed to sub-lethal concentration of Fe3O4 Nps are increased in T1 and T2. Biochemical characteristics such as protein, carbohydrate, and lipid in muscle, gill, and liver of Tilapia are higher in T1.
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