EVALUATION OF CYTOTOXICITY AND ANTI-CANCER ACTIVITY OF Solanum torvum STEM EXTRACT ON NORMAL VERO AND HUMAN BREAST CANCER MCF - 7 CELL LINE

Main Article Content

D. SHANTHI
K. RAJA
R. SARAVANAN
https://orcid.org/0000-0002-5376-3066

Abstract

Limited plants have been screened for their complete biological and pharmacological nature. In the present research investigation an attempt was made to decipher the medicinal value of aqueous extract of Solanum torvum (S.torvum) stem. Phytochemical screening of the aqueous extract of S.torvum stem in the present study ascertain the presence of flavonoids, phenols, saponins, alkaloids, coumarins, sterols, proteins and reducing sugars. Further, the potentiality of aqueous extract of S.torvum stem was assessed for its cytotoxic effect on Normal Vero cell line and anticancer activity on Human breast adenocarcinoma cell line by 3-(4,5dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay and compared with normal drug target doxorubicin. A 24-hour incubation cell proliferation study reduced the cell viability of MCF-7 breast cancer cell lines. In vitro studies on cytotoxicity analysis on Vero cell line revealed that the aqueous stem extract of S.torvum show mild toxicity and further it was found to be effective in the prevention of cell proliferation by MCF-7 cell lines.

Keywords:
Phytochemical analysis, doxorubicin, cell viability, tryphan blue, selectivity index

Article Details

How to Cite
SHANTHI, D., RAJA, K., & SARAVANAN, R. (2021). EVALUATION OF CYTOTOXICITY AND ANTI-CANCER ACTIVITY OF Solanum torvum STEM EXTRACT ON NORMAL VERO AND HUMAN BREAST CANCER MCF - 7 CELL LINE. UTTAR PRADESH JOURNAL OF ZOOLOGY, 42(9), 50-59. Retrieved from https://mbimph.com/index.php/UPJOZ/article/view/2111
Section
Original Research Article

References

Ashworth A, Christopher LJ, Reis-Filho S. Genetic interactions in cancer progressions and treatment. Cell. 2011;145(1):30-38.

Cancer Statistics.
Available:http://www.cancer.gov/about-cancer/what-is-cancer/statistics
Accessed on 21/04/2020.

Neves MP, Cravo S, Lima RT, Vasconcelos MH, Nascimento MS, Silva AM. Solid-phase synthesis of 21-hydroxychalcones. Effects on cell growth inhibition, cell cycle and apoptosis of human tumor cell lines. Bioorganic Medicinal Chemistry. 2012;20:25-33.

Iwamoto T. Clinical application of drug delivery systems in cancer chemotherapy: Review of the efficacy and side effects of approved drugs. Biological Pharmacology Bulletein. 2013;36:715‑718.

Rashid S, Ali N, Nafees S, Ahmad ST, Arjumand W, Hasan SK, Sultana S. Alleviation of doxorubicin‑induced nephrotoxicity and hepatotoxicity by chrysin in Wistar rats. Toxicology Mechanics Methods. 2013;23:337‑345.

Mahassni SH, Al-Reemi RM. Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (Lepidium sativum) seeds. Saudi Journal of Biological Science. 2013;20(2):131-139.

Solowey E, Lichtenstein M, Sallon S, Paavilainen H, Solowey E, Lorberboum-Galski H. Evaluating medicinal plants for anticancer activity. Science World Journal. 2014;1-12.

Kaunda JS, Zhang YJ. The Genus Solanum: An ethnopharmacological, phytochemical and biological properties review. Natural Products Bioprospecting. 2019;9(2):77-137.

Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants, National Institute of Science Communication. New Delhi.1996;20-50.

Jaiswal BS. Solanum torvum: A review of its traditional uses, phytochemistry and pharmacology. International Journal of Pharmacy and Biological Sciences. 2012;3(4):104 -111.

Lee AV, Oesterreich S, Davidson NE. MCF-7 cells-changing the course of breast cancer research and care for 45 years. Journal of National Cancer Institute. 2015;107(7):1-8.

Khatoon N, Jain P, Choudhary AK. Phytochemical studies on seed and leaf extract of Solanum torvum (Sw). Indo American Journal of Pharmaceutical Research. 2015;5:1649-1653.

Jaikumar B, Jasmine R. A review on a few medicinal plants possessing anticancer activity against human breast cancer. International Journal of Pharmaceutical Technology and Research. 2016;9(3): 333-365.

Harborne JB. Phytochemical methods. In: Chapman &, Hall. New York. 1984;4-5.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 1983;65:55-63.

Jauregui HO, Hayner NT, Driscoll JL, Williams- Holland R, Lipsky MH, Gallen PM. Trypan blue dye uptake and lactate dehydrogenase in adult rat hepatocytes freshly isolated cells, Cell Suspensions and Primary Monolayer Cultures. In-vitro. 1981;17:1100-1110.

Badisa RB, Darling-Reed SF, Joseph P, Cooperwood JS, Latinwo LM. Selective cytotoxic activities of two nov¬el synthetic drugs on human breast carcinoma MCF-7 cells. Anticancer Research. 2009;29:2993-2996.

Kalaiyarasi D, Manobharathi V, Mirunalini S. Development of nano drugs: A promising avenue for cancer treatment. Research Journal of Biotechnology. 2021;16(4):234-244.

Isha S, Monika T, Avinash R, Astha T. Antimicrobial, Antioxidant and bioactive compounds from four Folk medicinal plants of Solan, Himachal Pradesh, India. Research Journal of Biotechnology. 2021;16(4):44-51

Biswas J, Roy M, Mukherjee A. Anticancer drug development based on phytochemicals. Journal of Drug Discovery, Development and Delivery. 2015;2(1) :1-6.

Badmus JA, Ekpo OE, Hussein AA, Meyer M, Hiss DC. Cytotoxic and cell cycle arrest properties of two steroidal alkaloids isolated from Holarrhena floribunda (G. Don) leaves. BMC Complementary and Alternative Medicine. 2019;19:112-120

Kalebar VU, Hoskeri JH, Hiremath SV, AB Kalebar RV, Sonappanavar KL, Agadi BS. Pharmacognostical and phytochemical analysis of Solanum macranthum (Dunal) Fruits. J Pharmacognsy and Phytochemistry. 2019;8:284–290.

Kalebar VU, Joy H. Hoskeri, Shivaprakash V. Hiremath, Murigendra B. HiremathMB. In-vitro cytotoxic effects of Solanum macranthum fruit. Dunal Extract with Antioxidant Potential. Clinical Phytoscience. 2020;6:24- 29

Pandey S. In vivo antitumor potential of extract from different parts of Bauhinia variegate Linn. against b16f10 melanoma tumor model in c57bl/6 mice. Applied Cancer Research. 2017;37:33-39

Omran R, Al-Taee ZM, Hayder O Hashim, Al-Jassani MJ. Extraction of phenolic compounds as antioxidants from some plants and their cytotoxic activity against breast cancer cell line. Asian Journal of Pharmaceutical and Clinical Research. 2017;10(7):349-355.

Jayakumar K, Murugan.K. Evaluation of major phytochemicals in the leaves and fruits of Solanum mauritianum Scop.: A potential herbal drug. International Journal of Research in Ayurveda and Pharmacy. 2016; 7(2):58-60.

Kalebar VU, Hoskeri JH, Hiremath SV, Hiremath MB. In vitro antiproliferative effect of aqueous extract of Solanum macranthum fruits on MDA-MB-231 triple negative breast cancer cell line. Journal of Applied Biology and Biotechnology. 2020;8(1):28–32.

Panigrahi, Muthuraman MS , Natesan R, Pemiah B. Anticancer activity of ethanolic extract of Solanum torvum (Sw). International Journal of Pharmacy and Pharmaceutical Sciences. 2014;6 (1):93-98 .

Radhika M, Ramakrishnaiah H, Krishna V, Deepalakshmi AP, Naveen Kumar S. Cytotoxic activity of methanolic extract of Solanum erianthum. International Journal of Pharmacy and Pharmaceutical Science. 2015;7(2):106-108

Fekry MI, Ezzat SM, Salama MM, Alshehri OY, Al-Abd AM. Bioactive glycol alkaloides isolated from Solanum melongena fruit peels with potential anticancer properties against hepatocellular carcinoma cells. Science Reports. 2019;9:1-11.

Chung DM, Kim JH, Kim JK. Evaluation of MTT and Trypan blue assays for radiation induced cell viability test in HepG 2 cells, International Journal of Radiation Research. 2015;13(4):331-335.

Arulvasu C, Vasantha Suppriya S, Babu G. Anticancer activity of Pongamia glabra seed oil extract against selected human cancer cell lines. International Research Journal of Pharmacy. 2012;3(8):131-134

Deepa M, Darsan MB, Ramalingam C. In vitro evaluation of the antioxidant, anti-inflammatory and antiproliferative activities of the leaf extract of Excoecaria agallocha L. International Journal of Pharmacy and Pharmaceutical Sciences. 2016; 7(11):346- 352.

Wardihan, Rusdi M, Alam G, Lukmanand A, Manggau M. Selective cytotoxicity evaluation in anticancer drug screening of Boehmeria virgata (Forst) guill leaves to several human cell lines: HeLa, WiDr, T47D and Vero. Dhaka University Journal of Pharmaceutical Science. 2013;12(2):87-90.

Artun FT, Karagoz A , Ozcan G, Melikoglu G, Anil S, Kultur S, Sutlupinar N. In vitro anticancer and cytotoxic activities of some plant extract on HeLa and Vero cell lines. Journal of Balkan Union of Oncology. 2016;21(3):720-725

Senthilraja P, Kathiresan K. In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines study of marine yeast. Journal of Applied Pharmaceutical Science. 2015;5(3):80-84.

Zeinoddini S, Nabiuni M, Jalali H. The synergistic cytotoxic effects of doxorubicin and Viola odorata extract on human breast cancer cell line T47-D. Journal of Cancer Research and Therapeutics. 2019;15: 1073-1079.