Main Article Content
Contamination of soil surface by used lubricating oil is a common occurrence in most developing countries. These hydrocarbon pollutants have harmful effects on the environment and human beings. Using hydrocarbon degrading microbes can be an alternative green technology for remediation of contaminated soil. The purpose of the present work is to evaluate the effectiveness of microorganisms indigenous to the soil in remediating the soil pollutant. Used engine oil contaminated soil samples were collected from service stations and motor garages located in Kirathoor, Mankadu and Nithiravilai of Kanniyakumari District. Hydrocarbon utilizing bacteria was isolated by its lipase producing activity. Oil utilization efficiency is estimated by counting total cell number of microbes at 24 h and oil displacement assay. A total of thirteen hydrocarbanoclastic bacteria were isolated from Kirathoor, Mankadu and Nithiravilai workshops engine oil contaminated soil. Among the thirteen bacteria only seven are identified as lipase producing bacteria and are identified as Acenetobacter, Pseudomonas and Proteus. Maximum of oil utilization efficiency was observed in Acenetobacter and Pseudomonas species at 2% and 4% of oil supplementation respectively. Proteus and Acinetobacter species showed positive result in oil displacement assay indicating the secretion of biosurfactant to degrade spilled oil. From the present work it is concluded that biosurfactant producing bacteria can be used for remediating oil spill area.
Mohammed MA. Treatment techniques of oil-contaminated soil and water aquifers. International Conference on Water Resources & Arid Environment. 2004;1-11.
Dominguez-Rosado RE, Pichtel J. Phytoremediation of soil contaminated with used motor oil: Enhanced microbial activities from laboratory and growth chamber studies. Environmental Engineering Science. 2004;2:157-68.
Achuba FI, Peretiemo-Clarke BO. Effect of spent engine oil on soil catalase and dehydrogenase activities. International Agrophysics.2008;22:1-4.
Osubor CC, Anoliefo GO. Inhibitory effect of spent lubricating oil on the growth and respiratory function of Arachishypogaea L. Benin Science Dig. 2003;1:73–79.
Roling WFM, Milner MG, Jones DM, lee K, Daniel F, Swannell RJP, Head IM. Robust hydrocarbon degradation and dynamics of bacteria communities during nutrient- enhanced oil spill bioremediation. Applied and Environmental Microbiology. 2002;68(11):5548.
Lin J, Mandri T. Isolation and characterization of engine oil degrading indigenous microorganisms in KwaZulu-Natal, South Africa. African Journal of Biotechnology. 2007;6(1):23-27.
Bartha R. Biodegradation of petroleum in soil environment at low temperatures. Journal of Microbiology.1992;17:1652-1857.
Namkoong W, Hwang EY, Park JS, Choi 1Y. Bioremediation of diesel contaminated soil with composting. Environmental Pollution. 2002; 119: 23-31.
Boonchan S, Britz MC, Stanley GA. Degradation and mineralization of high molecular weight polycyclic aromatic hydrocarbons by defined fungal-bacterial co- cultures. Applied Environmental Microbiology. 2000;66 (3):1007-1019.
Abdulsalam S, Adefila SS, Bugaje IM, Ibrahim SC. Bioremediation of soil contaminated with used motor oil in a closed system. Journal of Bioremediation and Biodegradation. 2012;3(12): 1-7.
Morikawa M, Hirata Y, Imanaka, TA. A study on the structure-function relationship of lipopeptide biosurfactants. Biochimica et Biophysica Acta. 2000;1488:211–218.
Khouloud M. Barakat, Sahar WM. Hassan, Osama M. Darwesh. Egyptian Journal of Aquatic Research. 2017;43:205-211.
Stephen E, Emmanuel OE, Okpanachi OS, Emmanuel S, Temola OT, Musa K, et al. In-vitro study of biodegradation of spent lubricating oil by Aspergillusniger. Nature and Science. 2013;11 (10):40-44.
Deepa R, Vidhya A, Arunadevi S. Screening of bioemulsifier and biosurfactant producing streptomyces from different soil samples and testing its heavy metal resistance activity. International Journal of Current Microbiology and Applied Sciences. 2015;4:687-94.
Anuratha Pendse, Aruna K. Use of various screening methods for isolation of potential biosurfactant producing microorganisms from oil-contaminated soil sambles. Journal of pharmacy Research. 2018;12(4).
Venosa D, Zhu X, Suidan MT, Lee K. Guidelines for the bioremediation of marine shorelines and freshwater wetlands, U.S. Environmental Protection Agency. National Risk Management Research Laboratory Cincinnati, Ohio, Ohio, USA; 2001.
Ijah UJJ, Antai SP. The potential use of chicken-drop microorganisms for oil spill remediation. The Environmentalist. 2003;23:89–95.
Adenipekun CO, Isikhuemhen O S. Bioremediation of engine oil polluted soils by a tropical white rot fungus, Lentinus squarrosulus Mont. (Singer). Pakistan Journal of Biological Science. 2008;11(12):1634-1637.
Shukor MY, Hassan NAA, Jusoh AZ, Perumas N, ShamaaanNA, Mac Cormak WP, et al. Isolation and characterization of a Pseudomonas diesel degrading strain from Antartica. Journal of Environmental Biology. 2009;30(1):1-6.
Adenipekun CO, Ayanleye Oo, Oyetunji OJ. Bioremediation of soil contaminated by spent diesel oil using Pleurotuspulmonarius fries (Quelet) and its effects on the growth of Corchorus olitorius (L). Journal of Applied Biosciences. 2013;68:5366-5377.
Bola O Oboh, Matthew O IIori, Joseph O Akinyemi, Sunday A Adebusoye. Hydrocarbon degrading potentials of bacteria isolated from a Nigerian Bitumen (Tarsand) Deposit. Nature and Science. 2006;4(3).
Vijaya Kumar Gupta, Davender Kumar, Lalit Kumar, Sushil Nagar, Chand Raina, Rajinder Parshad. Archives of Applied Science Research. 2012;4(4):1763-1770.
Sunkar S, Valli Nahciyar. Endophytes: A prospective source of enzyme production. Journal of Pure and Applied Microbiology. 2012a;6(2):859-867.
Youssef NH, Duncan KE, Nagle DP, Savage KN, Knapp RM. Comparison of methods to detect biosurfactant production by diverse microorganisms. Journal of Microbiological Methods. 2004; 56:339–347.
Niraj Prasad, Sumitha Dasgupta, Mousumi Chakraborty, Smita Gupta. Earth and Environmental Science. 2017;78:1755 – 1315.
Akhavan Sepahi, Dejban GolpashaI, EmamiM, Nakhoda AM. Isolation and characterization of crude oil DEGRADING Bacillus sp., Iranian Journal of Environmental Health Science & Engineering. 2008;5(3):149-154.