DIFFERENTIAL PROTEIN EXPRESSION OF PROGLOTTIDS OF CYCLOPHYLLIDEAN CESTODE, Raillietina tetragona (MOLIN, 1858) INFECTING DOMESTIC CHICK, Gallus gallus
N. ACHAIAH
*
University Arts and Science College (Autonomous), Subedari, Dept of Zoology, Kakatiya University, Warangal, Telangana, India.
N. VIJAYA KUMAR
Department of Zoology, Kakatiya University, Warangal, Telangana, India.
*Author to whom correspondence should be addressed.
Abstract
Cestodes are endoparasites, and exhibit a great degree of difference in morphology and physiology within the same body. Raillietina tetragona infects domestic chick, Gallus gallus, the present study was designed to study the regionally specific protein expression in immature, mature and gravid regions by using SDS-PAGE. A total of 14 protein bands were observed ranging in the size from approximately10,250-95,720 KDa was detected in different proglottids under reducing SDS-PAGE condition, the molecular marker ladder of 170,130,95,72,55,43,34,26,17,10 KDa MW were used. The newly formed protein bands in mature region suggesting rapid reproductive activity, synthesis of proteins required for egg production. The disappeared bands in gravid region indicates the degenerative activities; i, e they are not involved in proliferation and scolex formation. The reason for variation is due to differential metabolic activity.
Keywords: Raillietina tetragona, Gallus gallus, SDS-PAGE, proglottids, cestode.