PHYTOCHEMICAL ANALYSES, In vitro ANTIOXIDANT AND ANTIBACTERIAL EFFICACY OF AQUEOUS EXTRACTS OF SEAWEEDS Enteromorpha intestinalis (L.) AND Gracilaria edulis (Gmelin) (Silva) COLLECTED FROM PULICAT LAKE, TAMIL NADU
UTTAR PRADESH JOURNAL OF ZOOLOGY, Volume 42, Issue 13,
Seaweeds are natural, abundant and vital bio resources available as a product of vast marine ecosystem. The green algae Enteromorpha intestinalis and red algae Gracilaria edulis were collected from Pulicat lake in Tamil Nadu and their respective aqueous extracts were prepared. Those extracts were screened for phytochemical availability and evaluated for in vitro antibacterial activity against five pathogenic bacteria viz., Vibrio parahaemolyticus, Staphylococcus aureus, Salmonella typhi, Escherichia coli and Vibrio alginolyticus. Both seaweed extracts were found to be effective against Salmonella typhi tested. The seaweed aqueous extracts were also tested for the antioxidant potential using the DPPH method and it is inferred that E. Intestinalis showed higher radical scavenging ability and the efficiency was found to be more than half of the known standard BHT. Therefore this study suggests the usage of seaweeds E. intestinalis and G. edulis for pharmaceutical and nutraceutical purposes.
- Enteromorpha intestinalis
- Gracilaria edulis
- antibacterial and antioxidant
How to Cite
Dring MJ. The biology of marine plants. Edward Arnold publishers Limited, London. 1982;199.
John DM. Biodiversity and conservation: an algal perspective. The phycologist.1994;38:3-15.
Phillips J. Marine macro algal biodiversity hotspots: why is there high species richness and endemism in southern Australian marine benthic flora? Biodiversity and Conservation. 2001;10(9):1555–1577..
Norziah MH, Ching CY. Nutritional composition of edible seaweed Gracilaria changgi. Food Chemistry. 2000;68: 69-76.
Smit AJ. Medicinal and pharmaceutical uses of seaweed natural products: A review. Journal of Applied Phycology. Journal of Applied Physiology. 2004;16:245-262.
Hemasudha TS, Thiruchelvi R, Balashanmugam P. Antioxidant, antibacterial, and anticancer activity from Marine Red Algae Gracilaria edulis. Asian Journal of Pharmaceutical and Clinical Research; 2019.
DOI: 10.22159/ajpcr.2019.v12i2.29883. ISSN: 0975-7538.
Cantillo-Ciau Z, Moo-Puc R, Quijano L, Freile-Pelegrin Y. The tropical brown alga Lobophora variegata: A Source of Antiprotozoal Compounds. 2010;8:1292–1304.
Gupta S, Abu-Ghannam N. Bioactive potential and possible health effects of edible brown seaweeds. Trends Food Sci. Techn. 2011;22:315-326.
Lincolon RA, Strupinski K, Walker JM. Bioactive compounds from algae. Life Chemistry. Reports, 8, 97-183. : Open Access Library Journal. 2014,1991;1(4).
Ayyad SN, Abdel-Halim OB, Shier WT, Hoye T.R. Cytotoxic Hydroazulene Diterpenes from the Brown Alga Cystoseira myrica. Z. Natur forsch. 2003;58c:33-38.
Banu GR. Studies on the bacteria Aeromona ssp. in farmed fish and water in Mymensingh region. An M.S. Thesis submitted to the Department of Fisheries Biology and Limnology, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, Bangladesh. 1996;95.
Rahman, MM. Efficacy of medicinal plants against bacterial fish pathogens. An M.S. Thesis, submitted to the Department of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh. 2005; 73.
Smith P, Hiney MP, Samuelsen OB. Bacterial resistance to antimicrobial agents used infish farming. Annual Review of Fish Diseases. 1994;4:273-313.
Kolanjinathan K, Ganesh P, Govindarajan M. Antibacterial activity of ethanol extracts of seaweeds against fish bacterial pathogens, European Review Medical Pharmacological Sciences. 2009;13:173-177.
Wijesinghe WAJP, Jeon YJ. Biological activities and potential industrial applications of fucose rich sulphated polysaccharides and fucoidans isolated from brown seaweeds: A review, Carbohydr. Polym. 2012;88:13-20.
Ravikumar S, Vinoth R, Selvan G.P. Bioactive potential of a Seagrass Syringodium isoetifolium against bacterial fish pathogens. Journal of Pharmacy Research. 2011;4(6):1854-1856.
Elnabris KJ, Elmanama AA, Chihadeh WN. Antibacterial activity of four marine seaweeds collected from the coast of Gaza Strip, Palestine. Mesopot. Journal of Marine Sciences. 2013;28(1):81–92.
Rao B V, Dr.Boominathan M. Antibacterial Activity of Silver Nanoparticles of Seaweeds. American Journal of Advanced Drug Delivery; 2015.
Shafay SME, Ali SS, Sheekh MME. Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria. Egyptian Journal of Aquatic Research; 2015.
El-Din SMM, El-Ahwany AMD. Bioactivity and phytochemical constituents of marine red seaweeds (Janiarubens, Corallina mediterranea and Pterocladia capillacea). Journal of Taibah University for Science. 2016;10:471–484.
Vimala T, Poonghuzhali TV. In vitro antimicrobial activity of solvent extracts of marine brown alga, Hydroclathrusclathratus (C. Agardh) M. Howe from Gulf of Mannar. Journal of Applied Pharmaceutical Science. 2017;7(04):157-162.
Li Y, Sun S, Pu X, Yang Y, Zhu F, Zhang S and Xu N. Evaluation of Antimicrobial Activities of Seaweed Resources from Zhejiang Coast, China. Sustainability. 2018;10:2158;.
Kalaivani P, Kavitha D, Vanitha V. A Review on Phytochemical and Pharmacological activities of Syringodium isoetifolium. International Journal of Research in Pharmaceutical Sciences; 2019.
Kavitha D, Kalaivani P, Vanitha V. A. review on phytochemicals and biological activities of seagrass. Journal of Critical Reviews.
ISSN- 2394-5125. 2020;7(7).
Nithya TG, Jayanthi J, Raghunathan MG. Phytochemical, antibacterial and GC MS analysis of a floating fern Salviniamolesta D.S. Mitchell. International Journal of Pharm Tech Research. 2015;8(9):85-90. (Scopus).
Nithya TG, Jayanthi J, Ragunathan MG. Antioxidant activity, total phenol, flavonoid, alkaloid, tannin, and saponin contents of leaf extracts of Salvinia molesta D. S. Mitchell .Asian J Pharmaceutical Clinical Research. 2016;9(1):200-203 (Scopus).
Vogel AL. Text book of practical organic chemistry, London: The English Language Book Society and Longman. 1978;1363.
Raman N. Phytochemical Technique. New Indian Publishing Agencies, New Delhi. 2006;19.
Bauer AW, Kirby WM, Sherries JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathnology. 1966;45:493–496. [PubMed] [Google Scholar]
Shen Q, Zhang B, Xu R, Wang Y, Ding X , Li P. Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01. Anaerobe, August. 2010;16(4):380-6.
SPSS. IBM SPSS statistics for windows version 22.0. Armonk: IBM Corp; 2010.
Singh AP, Kumar S. Applications of Tannins in Industry. Intech Open; 2019.
Minatel IO, Borges CV, Ferreira MI, Gomez, HAG, Chen CO, Lima GPP. Phenolic Compounds: Functional Properties, Impact of Processing and Bioavailability; 2017. Intech Open.
Lee JK, Jung DW, Eom SY, Oh SW, Kim Y, Kwak HS, kim YH. Occurrence of Vibrio parahaemolyticusin oysters from Korean retail outlets. Food Control. 2008;19:990– 994.
Nakaguchi Y. Contamination by Vibrio parahaemolyticusand its virulent strains in seafood marketed In Thail and Vietnam, Malaysia and Indonesia. Tropical Medicine and Health. 2013;41:95–102.
Jones JL, Lüdeke CH, Bowers JC, DeRosia-Banick K, Carey DH , Hastback W. Abundance of Vibrio cholerae, V. vulnificus, and V. Parahaemolyticusin oysters (Crassostrea virginica) and clams (Mercenariamer- cenaria) from LongIsl and sound. Appl. Environ. Microbiol. 2014;80:7667–7672.
Cruz CD, Hedderley D, Fletcher GC. Vibrio parahaemolyticus prevalence and distribution in New Zealand and shellfish: Along-termstudy. Applied and Environmental Microbiology; 2015.
DOI: 10.1128/AEM.04020-14[Epub ahead of print]
Letchumanan V, Yin WF, Lee LH , Chan KG. Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticusisolated from retail shrimps in Malaysia. Frontier in Microbiology. 2015;l6:33.
Tran L, Nunan L, Redman RM, Mohney LL, Pantoja CR, Fitzsimmons K, lightner DV. Determination of the infectious nature of the agent of acute hepatopancreaticnecros is syndrome affecting penaeid shrimp. Disease of Aquatic Organisms. 2013;105:45–55.
Bresee JS, Widdowson MA, Monroe SS, Glass RI. Food-borne viral gastroenteritis: Challenges and opportunities. Clinical Infectious Disease. 2002;35:748–753.
Kawatsu K, Ishibashi M, Tsukamoto T. Development and eval- uation of a rapid, simple, and sensitive immune chromatographic assay to detect thermostable direct hemolysin produced by Vibrio parahaemolyticusin enrichment cultures of stool specimens. Journal Clinical Microbiology. 2006;44:1821–1827.
Srivalli M, Devakumar D, Jayanthi J, Ragunathan MG. Efficacy of Ricinuscommunis L. leaf extract against Vibrio harveyi infection in a fresh water crab, Oziotelphusasenexsenex. International Journal of Biology, Pharmacy and Allied Sciences. 2014;3(4):549 –556.
Prabu DL, Sahu NP, Pal AK, Dasgupta S, Narendra A. Immunomodulation and interferon gamma gene expression in sutchi catfish, Pangasianodon hypophthalmus: Effect of dietary fucoidan rich seaweed extract (FRSE) on pre and post challenge period. Aquaculture Research. 2016;47(1):199-218.
Abstract View: 583 times
PDF Download: 8 times