PHARMACOLOGICAL ACTIVITY OF COMBINATION OF CURCUMIN AND VITAMIN C AGAINST OXIDATIVE STRESS INDUCED BY NAPROXEN IN MALE RATS
NAWRAS NOORI BASHBOOSH
Department of Biology, Faculty of Science, University of Kufa, Iraq.
*Author to whom correspondence should be addressed.
Abstract
The Aim: The goal of this study was to see if curcumin and vitamin C might protect male rats' blood from oxidative stress caused by naproxen using hematological criteria and antioxidant measures.
Study Design: The rats were aged 2.5-3 months and contemplated (200-250) gm. The purpose of this study was to see if Cur and Vitamin C might protect male rats against oxidative damage caused by NP, as well as hematological.
Place and Duration of Study: From April to October 2019, the experiment was conducted out on 48 male rats weighing between (200-250) gm and aged 2.5-3 months at the animal house of the faculty of science/university of Kufa.
Methodology: The 48 male Wister albino rats were separated into six groups at random; Group (1) NP was administered orally to rats at a dose of 40 mg/kg (as positive control). Group (2) cur at a dose of 150 mg/kg was given orally to the rats as a treatment. Group (3) During a 14-day period, rats were given 150 mg/kg of vitamin C by oral administration. Group (4) cur (150 mg/kg) and Vitamin C (150 mg/kg) were given orally to the rats as a treatment. Group (5) The oral administration of NP (40 mg/kg) plus Cur (150 mg/kg) plus Vit C (150 mg/kg) was performed on rats. Group (6) normal saline solution was given to the rats orally during the experiment (as negative control).
Results: Finishing the experiments, the findings displayed a considerable decrease (p<0.05) in the average of body weight, hemoglobin concentration (HB) and haematocrit ( HCT) while considerable increase (p<0.05) in the white blood cells(WBC) counts in the animals treated with NP compared to the control and other treated groups , while the results offered considerable increase (p <0.05) in the average of body contemplate , HB, HCT and a considerable decrease (p<0.05) in WBC rate in the animals treated with Cur, VitC, Cur+Vit C, Cur +Vit C+ Np compared the control group .
Conclusion: In conclusion, whereas naproxen has a harmful impact on the body by increasing oxidative stress, curcumin and vitamin C operate as powerful antioxidant supplements that reduce malonal di aldehyde (MDA) and boost overall antioxidant capacity by increasing superoxide dismutase (SOD) in the body.
Keywords: Curcumin, naproxen, oxidative stress, antioxidant