IN-SILICO PRIMER DESIGNING USING 18S rRNA GENES OF MONOGENEAN PARASITE, Thaparocleidus wallagonius
KALPANA SINGH *
Department of Zoology, D.N. (P.G.) College, Chaudhary Charan Singh University, Meerut- 250002, Uttar Pradesh, India.
PREETI ADHANA
Department of Zoology, D.N. (P.G.) College, Chaudhary Charan Singh University, Meerut- 250002, Uttar Pradesh, India.
VANDANA GARG
Department of Zoology, D.N. (P.G.) College, Chaudhary Charan Singh University, Meerut- 250002, Uttar Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Thaparocleidus wallagonius is a monogenean parasite and a fish-borne pathogen with a worldwide distribution. The present study focuses on in silico analysis of 18S rDNA of Thaparocleidus wallagonius collected from the fish Wallago attu from India. 18S rDNA serves as the source of taxonomic, standard reference sequences and systematic markers. The objective of genomics is the quantification, the collective characterization of the genes present in an organism, their influence, and interrelations on the organism. In this study, we designed 10 primers of the 18S rDNA sequence. 18S rRNA genes may be more useful in identifying the most efficient rRNA genes for genetic engineering and in understanding the relationship of the parasite with its host. The discovery of these primers will aid drug design studies, which could be a valuable tool for improving health of animal.
Keywords: Thaparocleidus wallagonius, primers, rDNA