LABORATORY EVALUATION OF THE ADULTICIDAL AND NYMPHICIDAL ACTIVITY OF Aspergillus niger In Bemisia tabaci (HEMIPTERA: ALEYRODIDAE)

ALYAA ABDUL-RIDHA HANASH *

College of Education for Pure Sciences, Wasit University, Iraq.

RANA JAAFAR ABED

a College of Education for Pure Sciences, Wasit University, Iraq.

ALAA NAJEE SALIH

College of Education for Pure Sciences, Wasit University, Iraq.

*Author to whom correspondence should be addressed.


Abstract

Whitefly, Bemisia tabaci (Gennadius), is a widespread polyphagous insect pest and a dangerous vector for many viruses that cause plant diseases. Farmers urgently need safe pesticides to protect their crops and plants, making biopesticide a good alternative to chemicals. Biopesticides are part of an integrated pest management program and offer a safer, more natural alternative to chemical pesticides. Since biocides were introduced, several pest management products have been released, some of which dominate the market. The current laboratory study tested the biological agent Aspergillus niger at three concentrations (0.25, 0.50, and 1.00 con/ml) to manage and reduce Bemisia tabaci population density in greenhouses and field crops. Laboratory tests showed that the two isolates were highly virulent against Bemisia tabaci nymphs and adults. Mortality was significantly different from controls in relation to isolate concentrations and time. Culture filtrate concentration and duration affected nymph and adult mortality. The 100% concentrations of both An1 and An2 were superior to the remaining concentrations, giving mortality in nymphs of B.tabaci 48.88 and 45.55 for isolates (An1 and An2) respectively, 42.21 and 36.66  for isolates (An1 and An2) respectively in adults stage. Effect of duration on post treatment mortality, highest mortality in nymphs and adults occurred 9 days after treatment, 74.44 , 56.66% in nymph and 53.33, 45.55 in adults for isolates (An1and An2) respectively. Concerning the interaction between concentration and duration, as is clear from Tables (1) and (2), the mortality was highest at 1.00% concentration. After 9 days of treatment, the isolates (An1, An2) achieved mortality 86.66, 80.0 in nymphs and 73.33%, 63.33% in adults, respectively. These findings demonstrate A. niger's biopesticide potential.

Keywords: Biological control, biopesticide, whitefly, Bemisia tabaci, Aspergillus niger


How to Cite

HANASH, A. A.-R., ABED, R. J., & SALIH, A. N. (2022). LABORATORY EVALUATION OF THE ADULTICIDAL AND NYMPHICIDAL ACTIVITY OF Aspergillus niger In Bemisia tabaci (HEMIPTERA: ALEYRODIDAE). UTTAR PRADESH JOURNAL OF ZOOLOGY, 43(24), 467–475. https://doi.org/10.56557/upjoz/2022/v43i243347

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References

Greenberg SM, Legaspi BC, Jones WA, Enkegaard A. Temperature-Dependent Life History of Eretmocerus eremicus (Hymenoptera: Aphelinidae) on Two Whitefly Hosts (Homoptera: Aleyrodidae). Environmental Entomology. 2000;29(4):851–860.

Xu C, Qiu BL, Cuthbertson AGS, Zhang Y, Ren SX. Adaptability of sweetpotato whitefly Bemisia tabaci (Hemipetera: Aleyrodidae) on seven marginal host plants. Int. J. Pest Manage. 2012;58:297-301.

Romba H, Olivier G, Samuel FD, Fidele T, Helene H, Laurence M, Fabrice V. Abundance of Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) and its parasitoids on vegetables and cassava plants in Burkina Faso (West Africa). Ecology and Evolution. 2018;8:6091–6103.

Osborn LS, Hoelmer K, Gerling D. Prospects for biological, damage ,control and managment . Audorer, u.k. Intercept. 1990;702.

Brown JK, Frohlich DR, Rosell RC. The sweetpotato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex?. Annual Review of Entomology. 1995;40(1):511-534.

Lapidot M, Polston JE. Biology and epidemiology of Bemisia-vectored viruses. In P. A. Stansly, Naranjo SE (Eds.), Bemisia: Bionomics and Management of a Global Pest. 2010;227–345.

Maan, A. Test of vitality of fungus Beauveria bassiana (Bals.) Vuill. on eggs and larvae of moth Figs Ephestia cautella (Walk.) (Lepidoptera: Pyralidae). Al-Mustansiriyah Journal of Science. 2017;28(3).

Cathrin PB, Ghanim M. Recent advances on interactions between the whitefly Bemisia tabaci and begomoviruses, with emphasis on tomato yellow leaf curl virus. In Plant Virus-Host Interaction: Molecular Approaches and Viral Evolution; Elsevier: Amsterdam, The Netherlands. 2014;79–103.

Al-Masoudi AD. Effect of plant varieties and chemical pesticides on the population density of the whitefly on the cucumber plant. Basra Journal of Agricultural Sciences. 2009;22(2): 115 – 124.

Kumar S, Singh A. Biopesticides: Present status and the future prospects. J. Fertilizers Pesticides. 2015;6:1-2.

DOI: 10.4172/2471-2728.1000e129

Samada LH, Usman SFT. Biopesticides as Promising Alternatives to Chemical Pesticides: A Review of Their Current and Future Status. OnLine Journal of Biological Sciences. 2020;20(2):66.76.

Chandler D, Bailey AS, Tatchel GM. l; Davidson ,G. & Greaves, J.et al., The development, regulation and use of biopesticides for integrated pest management. Philosophical Trans. Royal Society B: Biol. Sci. 2011;366:1987-1998.

DOI: 10.1098/rstb.2010.0390.

Glare T, Caradus J, Gelernter W, Jackson T, Keyhani N. et al. Have biopesticides come of age? Trends Biotechnol. 2012;30:250-258. DOI: 10.1016/j.tibtech.2012.01.003

Al-Fatlawi AAH. Evaluation efficacy of the biosynthetic silver nanoparticles by using filtrates and suspensions of some species of fungi in control of mosquito Culex quinquefasciatus. Master's thesis, University of Al-Qadisiyah , Iraq. 2021;17-18.

Mohanty SS, Prakash S. Comparative efficacy and pathogenicity of keratinophilic soil fungi against Culex quinquefasciatus larvae. Indian J Microbiol. 2010;50:299-302.

Mishra J, Tewari S, Singh S, Arora NK. Biopesticides: Where we stand. Plant Microbes Symbiosis: Applied Facets. 2015;9: 37-75.

DOI: 10.1007/978-81-322-2068-8

Kachhawa, D. Microorganisms as a biopesticides. J. Entomolgy Zool. Stud. 2017;5:468-473.

Tamanreet K, Jasleen K, Amarjeet K, Sanehdeep K. Larvicidal and growth inhibitory effects of endophytic Aspergillus niger on a polyphagous pest, Spodoptera litura . Phytoparasitica. 2016;44:465–476.

Kumari S, Verma D, Kumar D. Evaluation of Aspergillus niger as a Biocontrol Agent in the Insect Pest Management of Red Cotton Bug, Dysdercus koenigii (Heteroptera: Pyrrhocoridae) . Journal of Scientific Research. 2019;11 (2):235-247

Essien JP. Insecticidal potential of an orally administered metabolic extract of Aspergillus niger on Chrysomyachloropyga (Green bottle fly) Larvae. J Appl Sci Environ Multi-Gigabit Transceiver. 2004;8:45-8.

Siddhapara MR. Biology, seasonal incidence and management of red spider mite, Tetranychus urticae Koch in okra. Ph. D. Thesis (Unpublished) Junagadh Agricultural University, Gujarat; 2015.

Sabrine A, Kaouthar GL, Stephanie H, Georges L, Thierry H. An analysis of potential resistance of the phytophagous mite, Tetranychus urticae Koch (Acari: Tetranychidae) to four botanical pesticides. Biotechnol. Agron. Soc. Environ, 2015; 19(3):232-238.

Al-Zubaidi ANA, Al-Salami WM, Naas HAJ. Effect of different concentrations of the fungal filtrate of the fungus Aspergillus niger in nymphs and adults of the whitefly Bemisia tabaci genn. (Homoptea: Aleyroiddae). Al-Furat Journal of Agricultural Sciences. 2010;2(3) :176-182.

Kanika T, Rachna G, Monika G, Dhankhar SK. Dry weather: A crucial constraint in the field efficacy of entomopathogenic fungus Beauveria Bassiana against Tetranychus urticae Koch (Acari: Tetranychidae) Journal of Entomology and Zoology Studies. 2015;3(3): 287-291.

Serkan O, Nurcan AI. determination of control potentials and enzyme activities of Beauveria bassiana (bals.) vull. isolates against Tetranychus urticae koch (acari: tetranychidae), trakya University Journal of Natural Sciences. 2017;18(1):33-38.

Singh G, Prakash, S. Fungi Beauveria bassiana (Balsamo) metabolites for controlling malaria and filarial in tropical countries. Advan. in Biomed. Res. 2010;9:238-242.

Manal AR, Hany MH. Toxicity of Some Pesticides and Plant Extracts on Tetranychus urticae and its Predator, Phytoseiulus persimilis. International Journal of Zoological Research. 2019;5( 51):73-82.

Flore Z, Mustafa A, Inês S, Ibrahim C, Sara M. Inter-and intra-specific variationof spider mite susceptibility tofungal infections: implications for the long termsuccess of biological control.CC-BYNC-ND4.0 International licenseIt. 2019;1-19.

Ahmad F, Anwar W, Muhammad A, Ramsha B, Adnan A, Sajid A, Hafiz A, Ali K, Huma A. Muhammad SH. Infection mechanism of Aspergillus and Fusarium species against Bemisia tabaci. Mycopath. 2019;17(2):69-78.

Ullah MS, Lim UT. Laboratory bioassay of Beauveria bassiana against Tetranychus urticae (Acari: Tetranychidae) on leaf discs and potted bean plants. Experimental and Applied Acarology. 2015;65: 307–318.

AL Jubouri IJ, Abdul Sattar AA, Al- Anbaki NN. Cotton Pests and her Control Methods, National Program for the Development of Cotton Cultivation in Iraq, Indicative Bulletin. 2000;60.

Al-rabiei HAA, Salami WM. The effect of some isolated fungal suspensions of tetranychus urticae koch in her different life stages. Al furat Journal of Agricultural Sciences. 2016;8(4):151-8.

Abbas SS, Subaih AJ, Saleh YA. The Effects of Biological and Chemical Agents on the Management of Main Pests in Tomato Plant. Al-Qadisiyah Journal For Agriculture Sciences (QJAS). 2020;10(2):325-334.

Garcia AS, Messias CL, De Souza HML, Piedramuena AE, Patogenicidade de Metarhizium anisopliae var. anisopliae a Ceratitis capitata (Wied) (Diptera: Tephritidae). Revista Brasileira de Entomologia. 1984;28:421-424.

De la Rosa W, López FL, Liedo P. Beauveria bassiana as a pathogen of the Mexican fruit fly (Diptera: Tephritidae) under laboratory conditions. Journal of Economic Entomology. 2002;95(1):36-43.

Ahmed M, Ghazal I, Kerhili S, Rajab L. The pathogenicity of the fungus Beauveria bassiana (Bals.) Vuil. on adults and eggs of the two spotted spider mite Tetranychus urticae Koch in the laboratory. Arab Journal of Plant Protection. 2018;36(3):199-206

Negash R, Dawd M, Azerefegne F. Pathogenecity of Beauveria bassiana and Metarhizium anisopliae, to the two spotted spider mites, Tetranychus urticae, (Acari: Tetranychidae) at different temperatures and under greenhouse conditions. Ethiopian Journal of Agricultural Sciences. 2014;24: 51-58.

Tehri K. A review on reproductive strategies in two spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) J. Entomol. Zool. Stud. 2014;2:35–39.

Yesilayer A. Efficiency of two different entomopathogen fungi Beauveria bassiana and Purpureocillium lilacinum tr1 against Tetranychus urticae. Applied Ecology and Environmental Research. 2018;16(5):6077-6086.

Elhakim E, Mohamed O, Elazouni I. Virulence and proteolytic activity of entomopathogenic fungi against the twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Egyptian Journal of Biological Pest Control. 2020;30:30.

Samuel SLP, Tatiane CR, Ivani TO. Evander AF, Bárbara MC, Marcus AS, Victor HVR. Pathogenicity of Metarhizium anisopliae and Beauveria bassiana fungi to Tetranychus ludeni (Acari: Tetranychidae). Plant Parasitology; 2019.

DOI:10.1590/1808-1657000272018

Ortucu S, Iskender NA. Determination of control potentials and enzyme activation of Beauveria bassiana (Bals.) Vuil. isolates against Tetranychus urticae Koch (Acari: Tetranychidae). Trakya University Journal of Nature Sciences. 2017;18:33-38.

Perfect JR, Cox GM, Lee JY, Kauffman CA, De Repentigny L, Chapman SW, Morrison VA, Pappas P, Hiemenz JW, Stevens DA. The impact of culture isolation of Aspergillo species: A hospital-based survey of Aspergillosis. Clin. Infect. Dis. 2001;33:1824-1833.

Verweij PE, Brandt ME. Aspergillus, Fusarium, and other opportunitistic moniliaceous fungi. In PR Murray et al. (ed.) Manual of Clinical Microbiology, Ch. 121. 9th ed. ASM Press. Washington, DC. 2007;1802-1838.

Leipner J, Saller R. Systemic enzyme therapy in oncology:effect and mode of action. Drugs. 2000;59(4):769-780.

Pokorny D, Friedrich J, Cimerman A. Effect of nutritional factors on lipase biosynthesis by Aspergillus niger. Biotechnol Lett. 2010;16:363-366.

Mulimani PP. Bleach stable and alkalitolerant protease from Aspergillus flavus .Indian Journal of Microbiology. 2002;42(1):55-58.

Ismail M, Abdel-Sater MA. Fungi associated with the Egyptian cotton leafworm Spodoptera littoralis Boisdoval, Mycopathologia, 1993; 124:79-86.

Santos RRD, Muruci LNM, Santos LO, Antoniassi R, Silva JPL, Damaso MCT. Characterization of Different Oil Soapstocks and Their Application in the Lipase Production by Aspergillus niger under Solid State Fermentation. Journal of Food and Nutrition Research. 2014;2(9):561-566.

Gary S, Bryn D. Bioprospecting for Microbial Endophytes and Their Natural Products. Microbiology and Molecular Biology Reviews. 2003;67(4).

Lyakhovich VV, Vavilin VA, Zenkov NK, Menshchikova EB. Active defense under oxidative stress. The antioxidant responsive element. Biochemistry (Moscow). 2006; 71:962–974.

Ahmad F, Anwar W, Muhammad A, Ramsha B, Adnan A, Sajid A, Hafiz A, Ali K, Huma A. & Muhammad S. H. Infection mechanism of Aspergillus and Fusarium species against Bemisia tabaci Mycopath. 2019;17(2):63-72.

Reay DS, Hollingworth KA, Williams GE, Crozier FE, Jamieson DY, Beedell PL. A Darker Shade of Pale: Whiteness, the Middle Classes and Multi-ethnic Inner City Schooling’, Sociology. 2008;41(6):1041-60.

Petlamul W, Prasertsan P. Evaluation of strains of Metarhizium anisopliae and Beauveria bassiana against Spodoptera litura on the basis of their virulence, germination rate, conidia production, radiagrowth and enzyme activity. Mycobiology. 2012;40:111-116.

Mascarin GM, Kobori NN, Quintela ED, Ju´nior ID. The virulence of entomopathogenic fungi against Bemisia tabaci biotypeB (Hemiptera: Aleyrodidae) and their conidial production using solidsubstrate fermentation. Biological Control. 2013;66:209–218.