THE MOLECULAR STUDY OF LncRNA “NEAT2” FOR EARLY DIAGNOSIS OF TB IN POPULATION OF MADHYA PRADESH

PDF

Published: 2022-12-31

DOI: 10.56557/upjoz/2022/v43i243362

Page: 549-555


VEENA SINGH

Department of Biotechnology, SRK University, Bhopal, India.

DEEPAK BHARTI *

Department of Biotechnology, SRK University, Bhopal, India.

*Author to whom correspondence should be addressed.


Abstract

One of the deadliest infectious diseases in the world is tuberculosis (TB), a chronic disease brought on by the bacteria Mycobacterium tuberculosis. There is still no viable method for early screening, despite substantial advancements in tuberculosis detection methods and therapeutic strategies. Different cellular processes can hinder the growth of TB due to dysregulation of gene expression patterns connected to particular substances. Long non-coding RNAs (lncRNAs) are molecules that have been found to control the expression of protein-coding genes and take part in processes like cell cycle control, gene silencing, and cellular differentiation. It has been discovered that dysregulation of lncRNAs is linked to a variety of illnesses, including cancer and infectious diseases. Therefore, it is encouraging that lncRNAs have been identified as potential molecular indicators and therapeutic targets for tuberculosis. In present study. Human blood samples from the TB patients and healthy individuals from Sidhi District of Madhya Pradesh were collected. Total RNA were extracted from blood samples of using TRIzol Reagent (Takara) according to the manufacturer’s protocol. cDNA were prepared using synthesis kit (Biorad), In the present investigation gene expression of LncRNA “NEAT2” and IFNG were assessed  in WBCs of TB Patients and Healthy individuals, using  Real Time PCR technique. This study reported Lnc RNA NEAT2 up-regulation, which reflect significant involvement of this LncRNA in pathogenesis of TB and it may be one of the potential marker for the early diagnosis of TB.

Keywords: Tuberculosis, IFNG, molecular marker, LncRNA, NEAT2


How to Cite

SINGH, V., & BHARTI, D. (2022). THE MOLECULAR STUDY OF LncRNA “NEAT2” FOR EARLY DIAGNOSIS OF TB IN POPULATION OF MADHYA PRADESH. UTTAR PRADESH JOURNAL OF ZOOLOGY, 43(24), 549–555. https://doi.org/10.56557/upjoz/2022/v43i243362

Downloads

Download data is not yet available.

References

TB. Available at: www.who.int/en/news-room/factsheets/detail/tuberculosis. Accessed May 19, 2018.

Bayer R, Castro KG. Tuberculosis elimination in the United States—the need for renewed action. N Engl J Med 2017;377:1109–11.

Lyu M, Cheng Y, Zhou J, Chong W, Wang Y, Xu W, Ying B. Systematic evaluation, verification and comparison of tuberculosis‐related non‐coding RNA diagnostic panels. Journal of cellular and molecular medicine. 2021 Jan;25(1):184-202.

Li G, et al. Evaluation of a new IFN-γ release assay for rapid diagnosis of active tuberculosis in a high-incidence setting. Frontiers in Cellular and Infection Microbiology. 2017;7.

Lange C, Mori T. Advances in the diagnosis of tuberculosis. Respirology. 2010;15:220–240.

Clifford V, He Y, Zufferey C, Connell T, Curtis N. Interferon gamma release assays for monitoring the response to treatment for tuberculosis: A systematic review. Tuberculosis (Edinb). 2015;95:639–650.

Zur Bruegge J, Einspanier R, Sharbati S. A long journey ahead: Long non-coding RNAs in bacterial infections. Front Cell Infect Microbiol. 2017;7:95.

Mumtaz PT, et al. LncRNAs and immunity: watchdogs for host pathogen interactions. Biol Proced Online. 2017;19(3).

Wu T, Du Y. LncRNAs: From basic research to medical application. Int J Biol Sci. 2017;13:295–307.

Thum T, Condorelli G. Long noncoding RNAs and microRNAs in cardiovascular pathophysiology. Circ Res. 2015;116:751–762.

Weidle UH, Birzele F, Kollmorgen G, RÜger R. Long non-coding RNAs and their role in metastasis. Cancer Genomics-Proteomics. 2017;14:143–160.

Chandra Gupta S, Nandan Tripathi Y. Potential of long non-coding RNAs in cancer patients: From biomarkers to therapeutic targets. Int J Cancer. 2017;140:1955–1967.

Yan Y, et al. Circulating long noncoding RNA UCA1 as a novel biomarker of acute myocardial infarction. Biomed Res Int. 2016;8079372.

Fu Y, Xu X, Xue J, Duan W, Yi Z. Deregulated lncRNAs in B cells from patients with active tuberculosis. PLoS One. 2017;12: e0170712.

Wang Y, et al. Long noncoding RNA derived from CD244 signaling epigenetically controls CD8+ T-cell immune responses in tuberculosis infection. Proc Natl Acad Sci USA. 2015;112:E3883–3892.

Yi Z, Li J, Gao K, Fu Y. Identifcation of differentially expressed long non-coding RNAs in CD4+ T cells response to latent tuberculosis infection. J Infect. 2014;69:558–568.

Fu Y, Gao K, Tao E, Li R, Yi Z. Aberrantly expressed long non-coding RNAs In CD8+ T cells response to active tuberculosis. J Cell Biochem; 2017.

Yang X, et al. Microarray analysis of long noncoding RNA and mRNA expression profiles in human macrophages infected with Mycobacterium tuberculosis. Sci Rep. 2016;6:38963.

Tamgue O, Mezajou CF, Ngongang NN, Kameni C, Ngum JA, Simo US, Tatang FJ, Akami M, Ngono AN. Non-coding RNAs in the etiology and control of major and neglected human tropical diseases. Frontiers in Immunology. 2021;12.

Yao Y, Jiang Q, Jiang L, Wu J, Zhang Q, Wang J, Feng H, Zang P. Lnc-SGK1 induced by helicobacter pylori infection and high salt diet promote Th2 and Th17 differentiation in human gastric cancer by SGK1/Jun B signaling. Oncotarget. 2016;7(15):20549–20560.

Fathizadeh H, Hayat SMG, Dao S, Ganbarov K, Tanomand A, Asgharzadeh M, Kafil HS. Long non-coding RNA molecules in tuberculosis. Int J Biol Macromol. 2020;1:156:340-346.

Koch, Linda. Screening for lncRNA function. Nature Reviews Genetics. 2017;18.2:70- 70.