A Comparative Study on the Growth of Oyster Mushroom in Different Substrates


Published: 2023-01-22

DOI: 10.56557/upjoz/2023/v44i13393

Page: 74-80

I. Viji Margaret *

Zoology Department and Research Centre, Sarah Tucker College (Autonomous), Affiliated to Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, India.

l. Jeya Praba

Zoology Department and Research Centre, Sarah Tucker College (Autonomous), Affiliated to Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, India.

G. Sathiya

Zoology Department and Research Centre, Sarah Tucker College (Autonomous), Affiliated to Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, India.

*Author to whom correspondence should be addressed.


Mushrooms are cultivated worldwide for their taste, nutritional attributes, and potential application industries.  The edible oyster mushrooms Pleurotus florida and Hypsizygus ulmarius were selected for the present study. The different organic wastes such as paddy straw, coconut dry leaves, and palm dry leaves were used as the substrates for mushroom cultivation. Among the three substrates, paddy straw is a good substrate and provides a good yield of oyster mushrooms. Because paddy straw contains higher quantities of minerals and irons than other substrates which are needed for the growth of mushrooms. Pleurotus florida showed a total weight yield of 1.250 gm and Hypsizygius ulmarius was 1.300 gm Mycelium growth was very slow in the palm and coconut leaves. Meanwhile, mycelium was quickly grown in paddy straw. Cultivation of Pleurotus florida, Hypsizygius ulmarius oyster mushrooms on paddy straw was proven as the best substrate and follow the concept of conversion of agricultural waste into value-added products.

Keywords: Mushroom, substrates, paddy straw, coconut dry leaves, palm dry leaves Pleurotus florida, Hypsizygus ulmarius

How to Cite

Margaret, I. V., Praba, l. J., & Sathiya, G. (2023). A Comparative Study on the Growth of Oyster Mushroom in Different Substrates. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(1), 74–80. https://doi.org/10.56557/upjoz/2023/v44i13393


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Mushworld. Mushroom Growe's Handbook 1. Retrieved February 10, 2013, from National Symposium on Mushrooms, Thiruvananthapuram; 2004.

Shah ZA, Ashraf M, Ishtiaq MC. Comparative study on cultivation and yield performance of oyster mushroom (Pleurotus ostreatus) on different substrates (wheat straw, leaves and sawdust). Pak J Nutr. 2004;3:158–160.

Nigistie Ejigu, Baye Sitotaw, Solomon Girmay, Hirut Assaye. Evaluation of oyster mushroom (pleurotus ostreatus) production using water hyacinth (Eichhornia crassipes) biomass supplemented with agricultural wastes. International Journal of Food Science. 2022;2022:Article ID 9289043 DOI:https://doi.org/10.1155/2022/9289043

Dubey D, Dhakal B, Dhami K, Sapkota P, Rana M, Poudel NS, Aryal L. Comparative study on effect of different substrates on yield performance of oyster mushroom, GJBAHS. 2019;7:7.

Ambili S, Nitiya TP. Oyster mushroom cultivation- A study in Palakkad district, Kerela. International Journal of Management and Social Science Research Review. 2014;1:104-105.

Jandaik CL, Kapoor JN. Studies on cultivation of Pleurotus sajor-caju (Fr.) Singer. Mush. Sci. 1974;9(1): 667–672

Ram Naraian, Singh MP, Siya Ram. Supplementation of basal substrate to boost up substrate strength and oyster mushroom yield: An overview of substrates and supplements. Int. J. Curr. Microbiol. App. Sci. 2016;2016:5(5): 543-553.

Pokhrel CP, Yadav RKP, Ohga S. Effects of physical factors and synthetic media on mycelial growth of Lyophyllum decastes. Journal of Ecobiotech. 2009;1:046-050.

Adams A, Seecharran D, Ansari. Growth of oyster mushroom using sawdust and agriculture waste as substrates. Mushroom Research. 2022;31(1):73–80.

Hasan MT, Khatun MHA, Sajib MAM, Rahman MM, Rahman MS, Roy M, Miah MN, Ahmed KU. Effect of wheat bran supplement with sugarcane bagasse on growth, yield and proximate composition of pink oyster mushroom (Pleurotus djamor). American Journal of Food Science and Technology. 2015;3:6: 150-157.

Marlina L, Sukotjo S, Marsudi S. Potential of oil palm empty fruit bunch (EFB) as media for oyster mushroom. Pleurotus ostreatus Cultivation. Procedia. Chem. 2015;16:427–431.

Alananbeh KM, Bouqellah NA, Al Kaff NS. Cultivation of oyster mushroom Pleurotus ostreatus on date-palm leaves mixed with other agro-wastes in Saudi Arabia. Saudi. J. Biol. Sci. 2014;21:616–625.

Ananbeh K, Almomany A. Production of Oyster mushroom (Pleurotus ostreatus) on tomato tuff agro-waste. Dirasat Agric. Sci. 2008;35:133–138.

Bably Pearl Varghese, Pavithra Amritkumar. Comparative study on cultivation of oyster mushrooms using nutrition enhancing substrates. International Journal of Scientific Research in Research Paper. Biological Sciences. 2020;7(2):105-111.

Khandar RR, Vaishnav MV, Akbari LF, Andhania JH. Effect of various plant substrates on sporophore production of Pleurotus sajorcaju. In: Indian Mushrooms. Proceedings National Symposium on Mushrooms, Thiruvananthpuram; 1991.

Mathew J, Kothandaraman R, Joseph K. Cultivation of oyster mushroom on rubber processing factory waste – A possible solid waste utilization method. Indian Mushrooms. (Eds. Nair M C, Balakrishnan S and Gokulapalan C). Proceedings of national Symposium on Mushroom, Kerala Agricultural University, Trissur. 1991;97-99.

Gupta VK, Langer PN. Pleurotus florida for upgrading the nutritive value of wheat straw. Biological Wastes. 1988;23:57-64.

Chang ST, Lau OW, Cho KY. The cultivation and nutritional value of Pleurotus sajorcaju. Applied Microbiology and Biotechnology. 1981;12:58-62.

Poppe J. The fruit regulating to action of light and chemicals in the culture of P. ostreatus. Hort. Abst., 1974;44:868.

Imbernon M, Delmas J, Laborde J, Poitou N. Culture de P. ostreatus Sur substrates a base decorces. Mush. Sci. Industry. M.Sc. Thesis. Department of Plant Pathology, Faisalabad. 1977;9:175-197:50.

Khan SM, Ali MA. Cultivation of oyster mushroom (Pleurotus sp.) on cotton boll locules. Mush. Sci. 1981;11:691-695.

Abound AK. Potential of water hyacinth in ruminant nutrition in Tanzania; 2005. Retrieved June 20, 2012

Kimenju JW, Odera GO, Mutitu EW, Wachira PM, Narla R, Muiru WM. Suitability of locally available substrates for oyster mushroom (Pleaurotus oesteatus) Cultivation in Kenya; 2009.

Retrieved January 13, 2011.


Anne Sahithi Somavarapu Thomas, Sona Arivu, Vinodhini Shanmugam, Marttin Paulraj Gundupalli, Suksun Amornraksa. Paddy straw: an economical substrate for oyster mushroom (Pleurotus ostreatus) cultivation E3S Web of Conferences. 2022;355:02019:RI C 2022.

Zadrazil F. Cultivation of pleurotus. In S.T.Chang and W.A. Hayes (ed). The biology and cultivation of edible mushrooms. Pg.No:521-58, Academic Press, New York; 1978.

Aquino D, Barrio AD, Trach NX, Hai NT, Khang DN, Toan NT, Hung NV. Chapter 7: Rice Straw-Based Fodder for Ruminants. Editors. Gummert, M., N. V. Hung, P. Chivenge, B. Douthwaite. Rice straw-based fodder for ruminants. Springer. Cham; 2020.