Synthesis, Characterization and Biological Screening Study of Some New Mixed Ligand Complexes with Pt(II) Complex


Published: 2023-04-27

DOI: 10.56557/upjoz/2023/v44i73464

Page: 16-31

Wafa Abdel-Kazem Katie *

Department of Chemistry, College of Education for Girls, University of Kufa, Iraq.

Muna Abass Hadi

Department of Chemistry, College of Education for Girls, University of Kufa, Iraq.

*Author to whom correspondence should be addressed.


The Aim: The purpose of our study to synthesis of [6-(((Z)-1,5-dimethyl-2-phenyl-4-(((E)-4-(pyridin-2-yl)benzylidene)amino)-1,2-dihydro-3H-pyrazol-3-ylidene)amino)-3,3-dimethyl-7-oxo-4-thia-1azabicyclo[3.2.0]hept-5-ene-2-carboxylic acid] (L1) was new Schiff base and (E)-1-(2-((5-amino-2-hydroxyphenyl) diazenyl) phenyl)ethan-1-one (HL2) was azo ligand were mused to synthesize mixed ligand complexes of ions Co(II), Ni(II), Cu(II), Zn(II), Cd(II) Hg(II)and Pt(II).

Study Design: Preparation of new ligands and study of their biological activity on Cancer Cells.

Place and Duration of Study: The experimental Part carried out in Chemistry department- Lab of Inorganic chemistry.

Methodology: The spectroscopic data of the complexes suggest that their (1:1:1) (L1:M:HL2) is mole ratio. Also, the analytical studies for all new complexes have shown octahedral geometries, except for Pt (II) complex which suggested square planar geometry also its conductivity measurements show electrical properties

Results: All new compounds were analyzed The structure of these compounds has been investigated by using elemental analysis, FT-IR, UV-Vis spectrometric methods, 1H-NMR, mass spectra, atomic absorption, molar conductivity and magnetic susceptibility of metal complexes, and computational techniques to elucidate their chemical formulae.

Conclusion: The biological activity results showed the inhibitory effect for platinum(II) the complex. In this study the cytotoxicity of Pt(II) complex on human colon cancer cell line LS 174 T CRC was investigated and African green monkey kidney cells normal cells were MTT assay. The metal complexes showed selective High cytotoxicity against colorectal, this metal Complex excelled in halting proliferation of LS174T CRC cancer cells line with median inhibitory concentration (IC50) values of Pt(II) complex. The outcomes unquestionably point to the feasibility of utilizing them as antitumor drugs in the field of pharmacy colon cancer.

Keywords: Mix ligand, transition metal complexes, spectral studies, antitumor

How to Cite

Katie , W. A.-K., & Hadi , M. A. (2023). Synthesis, Characterization and Biological Screening Study of Some New Mixed Ligand Complexes with Pt(II) Complex. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(7), 16–31.


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Mgheer TH, Abdulmahdi BS, Mohammed LA. Bio-chemical study and preparation of some new mixed ligand complexes with novel (azo) ligand derived from 5-methyl imidazole and 1, 10-phenanthroline compounds. J Pharm Neg Results. 2023; 7135-46.

Althagafi I, El-Metwaly NM, Elghalban MG, Farghaly TA, Khedr AM. Synthesis of pyrazolone derivatives and their nanometer Ag(I) complexes and physicochemical, DNA binding, antitumor, and theoretical implementations. Bioinorg Chem Appl. 2018;2018:2727619. DOI: 10.1155/2018/2727619, PMID 29861710.

Mohammed LA, Hadi MA, Basim NA. Preparation and characterization of some complexes with new (Azo-Schiff base) ligand and study of complex as anticancer. Indian J Forensic Med Toxicol. 2020; 14(2):1298-305.

Mahmoud PDGS, Obaid DWA. Experimental studies on the effect of chlorpyrifos on rats. VI. Population of intestinal mast cells and hypersensitivity reactions. Al-Salam Journal for Biochemical and Medical Science. 2022;1(1):6-11. DOI: 10.55145/ajbms.2022.1.1.002

Ghyath SM, Ahmed AH, Kassim BM. Assessment of histopathological and hematological changes in mice treated with the aqueous extract of origanum (Driganum); 2022.

Kribaa L, Atia S, Boudehane A, Bader RMM, Gherraf N. Synthesis, characterization and antioxidant activity studies of new azo-compounds. J Biochem Technol. 2019;10(1):85.

Mahdi MA, Jasim LS, Mohamed MH. Synthesis, spectral and biological studies of Co(III), Ni(II), and Cu(II) complexes with new heterocyclic ligand derived from azo dye. Syst Rev Pharm. 2021;12(1):426-34.

Kadhim ZY, Seewan AN, Abd MT, Saud HR. Synthesis, Characterization, antibacterial Screening and application on the wool fabric of new bis-azo compounds derived from 4, 4’-Diaminodiphenylmethane. Int J Pharm Res. 2020;12(3):402-7.

Majeed WH, Baqer SR, Alsahib SA. Synthesis and biological study of some transition metal ions complexes of Schiff-Mannich base derived from 2-Amino-5- Mercpto-1,3,4 thiadiazole. J Med Chem Sci. 2023;6:789-802.

Buldurun K, Turan N, Savcı A, Çolak N. Synthesis, structural characterization and biological activities of metal (II). J Saudi Chem Soc. 2019;23(2):205-14. DOI: 10.1016/j.jscs.2018.06.002

Radha VP, Jone Kirubavathy SJ, Chitra S. Synthesis, characterization and biological investigations of novel Schiff base ligands containing imidazoline moiety and their Co(II) and Cu(II) complexes. J Mol Struct. 2018;1165:246-58.

DOI: 10.1016/j.molstruc.2018.03.109

Obaid MI, Jaafar WA. Formation, characterization and thermal study of novel Schiff base ligand from sulfonic acid and its complexes with Co(II), Ni(II), Cu(II), Zn(II) and Hg(II) type NO, chemical methodologies. 2022;6:457-62.

Hassan SA, Lateef SM, Majeed IY. Structural, Spectral and Thermal studies of new bidentate Schiff base ligand type (NO) derived from mebendazol and 4-aminoantipyrine and it’s metal complexes and evaluation of their biological activity. Res J Pharm Technol. 2020;13(6):3001-6. DOI: 10.5958/0974-360X.2020.00531.4

Al-Adilee KJ, Alshamsi HAH, Dawood MN. Synthesis, Spectral Characterization and photo Thermal Decomposition Studies of New Hetrocyclic Azo Dye Compound Derived from imidazole with Some Transition Metal Complexes. Res J Pharm Biol Chem Sci. 2016;7:2882-905.

EMMAN, J, MOHAMMED LA, ABBAS KK. Preparation, characterization and study of the biological screening of The AU (III) complex with novel (azo-Schiff base) ligand. Uttar Pradesh J Zool. 2022;43(12): 1-15.

Salih BM, Hadi MA. Synthesis, Identification and Spectral Studies of Some New Schiff base ligand Complexes Contained 3, 4-Diaminobenzophenone and pyridoxal hydrochloride compounds. J KUFA for Chem Sci. 2022;2(9).

Hadi MA, Kareem IK. synthesis, Characterization and Spectral Studies of a new Azo-Schiff base Ligand Derived from 3, 4-diamino benzophenone and its Complexes with Selected Metal Ions. Res J Adv Sci. 2020;1(1).

Al-Zaidi BH, Ismail AH, AN, Naseaf, Khamis W. Preparation, characterization and biological activity of new tridentate imine- ligand (H2L) and its metal complexes. Asian J Chem. 2018;30:1157-64.

Hadi MA, Kareem IK, Atban AK. Synthesis and characterization of novel metal complexes with new Schiff base ligand derived from 6-amino pencillic acid and toxicological studies of its complex with AU (III) on human cells for colon cancer LS-174; 2021.

Hadi M, A. Synthesis and Characterization of some metal ions Complexes with new Schiff base and Azo-Schiff base ligands and anticancer activity study for Pt(II) and Au(III) Complexes. PH .D [thesis]. Kufa University; 2020.

Mahdi SH, Karem LA. Synthesis, spectral and biochemical studies of new complexes of mixed ligand Schiff base and anthranilic acid. Orient J Chem. 2018;34(3):1565-72. DOI: 10.13005/ojc/340349

Taaima AN. Preparation and characterization of new chelate complexes derived from ligand azo_azomethine [M.Sc. thesis]. Kufa University; 2021.

Jasim EJ. Synthesis, characterization of some transition metal complexes novel Ligands derived from Azo and Azo- Schiff base and study the Biological activity of gold (III) complexes [Ph.D. thesis]. Kufa University; 2022.

Reda M, A, Mohammed LA. Preparation, characterization, and studied biological effect as antioxidant of azo compound and schiff base complexes. J Surv Fish Sci. 2023;10(3S):1144-56.

Rasoul WMR, Mahmood SJ. Evaluation of the efficacy of Brassica oleracea L. alcoholic extract on the levels of D3 and antioxidants in albino male rats dosed with paracetamol. Uttar Pradesh J Zool. 2022; 43(6):72-7.

AlBaheley NA, Fahad TA, Ali AA. Synthesis, spectroscopic, and thermal studies of azo compounds from luminol and procaine with acetylacetone and their complexes. J Phys Conf S. 2021;2063(1).

DOI: 10.1088/1742-6596/2063/1/012016 (Vol. 2063, No. 1, p. 012016) . IOP Publishing.

Ghafil RA, Alrazzak NA, Aljamali NA. Synthesis of triazole derivatives via multi components reaction and studying of (organic characterization, chromatographic behavior, chem-physical properties). Egypt J Chem. 2020;63(11):4163-74. DOI: 10.21608/ejchem.2020.23541.2399

Abdulghani SM, Al-Rawi MS, Tomma JH. Synthesis of New 1, 2, 4-triazole Derivatives with Expected Biological Activities. Chem Methodol. 2022;6(1): 59-66.

Cimerman Z, Miljanić S, Galić N. Schiff bases derived from aminopyridines as spectrofluorimetric analytical reagents. Croat Chem Acta. 2000;73(1):81-95.

Housecroft CE. AG Sharpe inorganic chemistry, England. Pearson. 2005;579: 27.

Mahmood NA, Sahib MN. Chemo – spectral and biological studying of new ligands. Res J Pharm Biol Chem Sci. RJPBCS. Page. 2017;8(3):674.

Vidyavathi GT, Kumar BV, Raghu AV, Aravinda T, Hani U, Murthy HCA, et al. Punica granatum pericarp extract catalyzed green chemistry approach for synthesizing novel ligand and its metal (II). J Mol Struct. 2022;1249:131656.

DOI: 10.1016/j.molstruc.2021.131656

Mahmoud WH, Omar MM, Sayed FN, Mohamed GG. Synthesis, characterization, spectroscopic and theoretical studies of transition metal complexes of new Nano Schiff base derived from L‐histidine and 2‐acetylferrocene and evaluation of biological and anticancer activities. Appl Organomet Chem. 2018;32(7):e4386. DOI: 10.1002/aoc.4386

Mahmood NA, Farhan ZM. Anticancer study of innovative macrocyclic formazan compounds from trimethoprim drug. Egypt J Chem. 2023;66:1. DOI:10.21608/EJCHEM.2022.132514.5852

Tajudeen SS, Kannappan G. Schiff base–copper (II) complexes: synthesis, spectral studies and anti-tubercular and antimicrobial activity. Indian J Adv Chem Sci. 2016;4(1):40-8.

Mohammed MK. Synthesis, spectral properties and biological studies of new schiff-azo ligand derived from 5- amino-2-phenyl -4h-pyrazol-3-onead its complexes with metal ions, M. SC [thesis]. Kufa University; 2022.

Sundholm D, Dimitrova M, Berger RJF. Current density and molecular magnetic properties. Chem Commun (Camb). 2021; 57(93):12362-78. DOI: 10.1039/d1cc03350f, PMID 34726205.

Shaygan S, Pasdar H, Foroughifar N, Davallo M, Motiee F. Cobalt (II) complexes with Schiff base ligands derived from terephthalaldehyde and Ortho-substituted anilines: Synthesis, characterization and antibacterial activity. Appl Sci. 2018;8(3): 385. DOI: 10.3390/app8030385

Salih B, M. Preparation and characterization of some metal elements complexes with two new schiff base and azo- schiff base ligands and study the biological inhibition for Au(III) complexes [M.Sc. thesis]. Kufa University; 2022.

Hadi MA, Mohammed MS, Kadhium AJ. Synthesis, characterization and spectral studies and Biological screening study of Transition Metal complexes with new Heterocyclic Ligand Derived from pyridoxal hydrochloride. Syst Rev Pharm. 2021; 12(1):371-83.

Shiju C, Arish D, Kumaresan S. Novel water soluble Schiff base metal complexes: Synthesis, characterization, antimicrobial-, DNA cleavage, and anticancer activity. J Mol Struct. 2020; 1221:128770. DOI: 10.1016/j.molstruc.2020.128770

Kadhim SM, Mahdi SM. Preparation and characterization of new (halogenated azo-Schiff) ligands with some of their transition metal ions complexes. Iraqi J Sci. 2022;63(8):3283-99.DOI: 10.24996/ijs.2022.63.8.4

Al-Daffay RKH, Al-Hamdani AAS. Synthesis and characterization of some metals complexes with new Acidicazo ligand 4-[(2-amino-4-phenylazo)-methyl]-cyclohexane carboxylic acid. Iraqi J Sci. 2022;63(8):3264-75.

DOI: 10.24996/ijs.2022.63.8.2

Aparna J, Pillai P, Ampili M. Antibacterial activity OF Clerodendrum infortunatum L. AND Vanda spathulata (L.) Spreng. AGAINST Escherichia coli AND Klebsiella sp. Uttar Pradesh J Zool. 2021;637-44.

Mattigatti S, Datkhile K. Synthesis, characterization and application of green synthesized ag nps in endodontic therapy to reduce microbial biofilm. Uttar Pradesh J Zool. 2021;42(12):25-30.

Jasim E, J, Mohammed LA. Preparation and Characterization of complexes with 2-amino pyrimidine and 4-methyl imidazole ligand and Study of Biological screening of the Au(III) Complex. Egypt J Chem. 2022;65(11):665-75.

Vaidya VV. The Effects of heavy metal contamination on antioxidant enzyme activity and oxidative stress in the earthworm perionyx excavates. Uttar Pradesh J Zool. 2023;44(3):59-65. DOI: 10.56557/upjoz/2023/v44i33421